Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
권호 표지
DOI QR코드

DOI QR Code

Neuroprotective Effects of Cervi Cornu in MPP+ Treated SH-SY5Y Cells

MPP+로 유도된 신경 독성에 대한 녹각의 보호 효과

  • Yeo, Sujung (Department of Meridian & Acupoint, College of Korean Medicine, Sang Ji University)
  • 여수정 (상지대학교 한의과대학 경혈학교실)
  • Received : 2020.04.28
  • Accepted : 2020.05.28
  • Published : 2020.06.27
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : Parkinson's disease, a progressive neurodegenerative disease, is caused by the loss of dopaminergic neurons in the substantia nigra. There is no clear treatment or remedy for Parkinson's disease; therefore, the development of novel therapies related to anti-inflammatory and antioxidant effects is required. This study was performed to evaluate the neuroprotective effect of water extracts from Cervi Cornu (CC) in dopaminergic cells. Methods : We studied effects of CC on apoptosis, cell death and inflammation in SH-SY5Y neuroblastoma cells treated by methylpyridinium ion (MPP+). SH-SY5Y cell line was treated with CC for 24 hours and then 500 μM MPP+ for 18 hours. Results : Cervi Cornu treatment inhibited the decrease in tyrosine hydroxylase (TH) expression and decreased the activation of inflammatory factors mitochondrial cytochrome C oxidase (COX2) and inducible NO synthase (iNOS) against MPP+ neurotoxicity. Apoptosis factors BCL2 associated X, apoptosis regulator (BAX) levels were decreased and B-Cell CLL/Lymphoma 2 (BCL2) levels were increased. Conclusions : These results suggest that CC treatment had neuroprotective effects in the SH-SY5Y neuroblastoma cells against toxicity induced by MPP+. The results suggest new possibilities of CC for the treatment of Parkinson's disease.

Keywords

References

  1. Tolosa E, Wenning G, Poewe W. The diagnosis of Parkinson's disease. Lancet Neurol. 2006 ; 5(1) : 75-86. https://doi.org/10.1016/S1474-4422(05)70285-4
  2. Albanese A. Diagnostic criteria for Parkinson's disease. Neurol Sci. 2003 ; 24 Suppl 1 : S23-6. https://doi.org/10.1007/s100720300032
  3. Frank C, Pari G, Rossiter JP. Approach to diagnosis of Parkinson disease. Can Fam Physician. 2006 ; 52 : 862-8.
  4. Zheng LT, Ryu GM, Kwon BM, Lee WH, Suk K. Anti-inflammatory effects of catechols in lipopolysaccharide-stimulated microglia cells: inhibition of microglial neurotoxicity. Eur J Pharmacol. 2008 ; 588(1) : 106-13. https://doi.org/10.1016/j.ejphar.2008.04.035
  5. Tasaki Y, Makino Y, Ohta S, Hirobe M. 1-Methyl-1,2,3,4-tetrahydroisoquinoline, decreasing in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-treated mouse, prevents parkinsonism-like behavior abnormalities. J Neurochem. 1991 ; 57(6) : 1940-3. https://doi.org/10.1111/j.1471-4159.1991.tb06407.x
  6. Salum C, Raisman-Vozari R, Michel PP, Gomes MZ, Mitkovski M, Ferrario JE, et al. Modulation of dopamine uptake by nitric oxide in cultured mesencephalic neurons. Brain Res. 2008 ; 1198 : 27-33. https://doi.org/10.1016/j.brainres.2007.12.054
  7. Hauser RA, Freeman TB, Snow BJ, Nauert M, Gauger L, Kordower JH, et al. Long-term evaluation of bilateral fetal nigral transplantation in Parkinson disease. Arch Neurol. 1999 ; 56(2) : 179-87. https://doi.org/10.1001/archneur.56.2.179
  8. Reichmann H. Modern treatment in Parkinson's disease, a personal approach. J Neural Transm (Vienna). 2016 ; 123(1) : 73-80. https://doi.org/10.1007/s00702-015-1441-1
  9. Fahn S. The history of dopamine and levodopa in the treatment of Parkinson's disease. Mov Disord. 2008 ; 23 Suppl 3 : S497-508. https://doi.org/10.1002/mds.22028
  10. Cacabelos R. Parkinson's Disease: From Pathogenesis to Pharmacogenomics. Int J Mol. 2017 ; 18(3) : 551. https://doi.org/10.3390/ijms18030551
  11. Chen H, Fang J, Li F, Gao L, Feng T. Risk factors and safe dosage of levodopa for wearing-off phenomenon in Chinese patients with Parkinson's disease. Neurol Sci. 2015 ; 36(7) : 1217-23. https://doi.org/10.1007/s10072-015-2078-4
  12. Tolosa E, Marti MJ, Valldeoriola F, Molinuevo JL. History of levodopa and dopamine agonists in Parkinson's disease treatment. Neurology. 1998 ; 50(6 Suppl 6) : S2-S48. https://doi.org/10.1212/wnl.50.6_suppl_6.s2
  13. Hwang JS, Seo BI, Park JH, Roh SS, Kim MR, Kim SM, et al. Effect of Cervi Cornu On treatment of Osteoporosis in Ovariectomized Rats. Kor J Herbology. 2010 ; 25(2) : 1-10. https://doi.org/10.6116/KJH.2010.25.2.001
  14. Kim HD, Lee EY, Park JY, Seo KH, Lee KH, Choi J, et al. Protective effect of ethanolic extract of antler-shaped Ganoderma lingzhi against oxidative stress in PC12 neuronal cell line. J Mushrooms. 2018 ; 16(3) : 213-7.
  15. Kim MJ, Cho SY, Park EM, Yoon SH. Effect of Old Antler Extracts on the Benzo(a)pyrene-Induced Hepatotoxicity in Rats. J Korean Soc Food Sci Nutr. 1993 ; 22(4) : 412-7.
  16. Gonzalez-Polo RA, Soler G, Fuentes JM. MPP+: mechanism for its toxicity in cerebellar granule cells. Mol Neurobiol. 2004 ; 30(3) : 253-64. https://doi.org/10.1385/MN:30:3:253
  17. Fall CP, Bennett JP, Jr. Characterization and time course of MPP+ -induced apoptosis in human SH-SY5Y neuroblastoma cells. J Neurosci Res. 1999 ; 55(5) : 620-8. https://doi.org/10.1002/ (SICI)1097-4547(19990301)55:5<620::AID-JNR9>3.0.CO;2-S
  18. Chen J, Sun J, Jiang J, Zhou J. Cyanidin Protects SH-SY5Y Human Neuroblastoma Cells from 1-Methyl-4-Phenylpyridinium-Induced Neurotoxicity. Pharmacology. 2018 ; 102(3-4) : 126-32. https://doi.org/10.1159/000489853
  19. Wang T, Pei Z, Zhang W, Liu B, Langenbach R, Lee C, et al. MPP+-induced COX-2 activation and subsequent dopaminergic neurodegeneration. FASEB J. 2005 ; 19(9) : 1134-6. https://doi.org/10.1096/fj.04-2457fje
  20. Teismann P, Vila M, Choi DK, Tieu K, Wu DC, Jackson-Lewis V, et al. COX-2 and neurodegeneration in Parkinson's disease. Ann N Y Acad Sci. 2003 ; 991 : 272-7. https://doi.org/10.1111/j.1749-6632.2003.tb07482.x
  21. Hsieh YC, Mounsey RB, Teismann P. MPP(+)-induced toxicity in the presence of dopamine is mediated by COX-2 through oxidative stress. Naunyn Schmiedebergs Arch Pharmacol. 2011 ; 384(2) : 157-67. https://doi.org/10.1007/s00210-011-0660-8
  22. Chae SW, Kang BY, Hwang O, Choi HJ. Cyclooxygenase-2 is involved in oxidative damage and alpha-synuclein accumulation in dopaminergic cells. Neurosci Lett. 2008 ; 436(2) : 205-9. https://doi.org/10.1016/j.neulet.2008.03.031