한국식품위생안전성학회지 (Journal of Food Hygiene and Safety)

  • 제35권3호
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  • Pages.284-289
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  • 2020
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  • 1229-1153(pISSN)
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  • 2465-9223(eISSN)
한국식품위생안전성학회 (The Korean Society of Food Hygiene and Safety)
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까마귀쪽나무 열매 추출물의 면역증가 및 뼈 건강 효과

Immunostimulatory and Bone Health-Promoting Activities of Litsea japonica Fruit Extract

  • 박성진 (충남대학교 농업생명과학대학 생물환경화학과) ;
  • 강준철 ((주)하람중앙연구소) ;
  • 이다영 ((주)하람중앙연구소) ;
  • 조주현 ((주)하람중앙연구소) ;
  • 윤민호 (충남대학교 농업생명과학대학 생물환경화학과)
  • Park, Sung-Jin (Department of Bio-Environmental Chemistry, College of Agriculture and Lifesciences, Chungnam National University) ;
  • Kang, Jun-Chul (Haram Central Research Institute Co., Ltd) ;
  • Lee, Da-young (Haram Central Research Institute Co., Ltd) ;
  • Cho, Ju-Hyun (Haram Central Research Institute Co., Ltd) ;
  • Yoon, Min-Ho (Department of Bio-Environmental Chemistry, College of Agriculture and Lifesciences, Chungnam National University)
  • 투고 : 2020.03.20
  • 심사 : 2020.04.21
  • 발행 : 2020.06.30
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초록

본 연구는 까마귀쪽나무 열매 추출물이 대식세포인 RAW264.7세포에서의 면역증가 효능과 조골세포인 MC3T3-E1 세포에서 뼈건강 효능에 미치는 효과를 확인하고자 하였다. 면역증가 효능을 알아보기 위해서 까마귀쪽나무열매 열수추출물(LJF-W)과 70% 에탄올추출물(LJF-70E)을 저농도(10 ㎍/mL), 중농도(100 ㎍/mL) 및 고농도(1,000 ㎍/mL)로 각각 사용하였고, 양성대조군으로는 홍삼 진세노사이드(Rg1+Rb1+Rg3 5.5 mg/g)를 이용하여 실험을 진행하였다. 실험 결과 열수추출물(LJF-W)과 70%에탄올추출물(LJF-70E) 처리군에서 NO 생성량이 무처리구 그룹에 비하여 통계적으로 유의하게 증가하였다. 염증성 cytokine인 TNF-α, IL-6, IL-1β의 생성량은 열수추출물(LJF-W)에서 무처리구에 비해 통계적으로 유의하게 증가하였으나, 70% 에탄올추출물(LJF-70E)에서는 차이가 없음을 관찰할 수 있었다. 또한 뼈건강 효능을 확인하기 위해서 까마귀쪽나무열매 추출물에 의한 조골세포인 MC3T3-E1세포의 세포증식능을 확인한 결과, 열수추출물과 70%에탄올추출물 처리군에서 조골세포 증식능이 무처리구에 비하여 통계적으로 유의하게 증가하였다. 이 결과를 통해 까마귀쪽나무열매 열수추출물(LJF-W)이 면역증가와 뼈건강에 대한 효능성분으로 가능성이 있음을 확인하였다.

This study was performed with hot water extract (LJF-W) and 70% ethanol extract (LJF-70E) of Litsea japonica fruit to investigate the immunostimulatory activity and bone health-promoting effect of L. japonica fruit. The production of pro-inflammatory mediator (nitric oxide) and pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β) in murine macrophage RAW264.7 cells were estimated to examine the immunostimulatory activity of the fruit extracts. The immunostimulatory activity of LJF-W was higher than that of positive control (geinsenosides). However, there was no effect in LJF-70E. Futhermore, both LJF-W and LJF-70E appeared to stimulate the proliferation of MC3T3-E1 cells, proving the effect as a bone health agent. From this result it could be presumed that L. japonica fruit extracts not only stimulate the immune system, but also the ability to promote bone health.

키워드

참고문헌

  1. Min, B.S., Lee, S.Y., Kim, J.H., Kwon, O.K., Park, B.Y., An, R.B., Lee J.K., Moon, H.I., Kim, T.J., Kim, Y.H., Joung, H., Lee, H.K., Lactones from the leaves of the Litsea japonica and their anti-complement activity. J. Nat. Prod., 66, 1388-1390 (2003). https://doi.org/10.1021/np030227i
  2. Takeda, K., Sakurawi, K. Ishii, H., Components of the Lauraceae family-l. New lactonic compounds from Litsea japonica. Tetrahedron, 28, 3757-3766 (1972). https://doi.org/10.1016/S0040-4020(01)93822-0
  3. Kandulski, A., Selgrad, M., Malfertheiner, P., Helicobacter pylori infection: a clinical overview. Dig Liver Dis., 40, 619-626 (2008). https://doi.org/10.1016/j.dld.2008.02.026
  4. Jimenez-Perez, N.C., Lorea-Hernandez, F.G., Jankowski, C.K., Reyes-Chilpa, R., Essential oils in mexican bays (Litsea spp., Lauraceae): Taxonomic assortment and ethnobotanical implications. Econ. Bot., 65, 178-189 (2011). https://doi.org/10.1007/s12231-011-9160-5
  5. Jang, M., Lim, T.G., Hong, H.D., Rhee, Y.K., Kim, K.T., Lee, E.J., Lee, J.H., Lee, Y.J., Kim, Y.B., Cho, C.W., Immunostimulatory activities of a high molecular weight fraction from Cynanchum wilfordii Radix obtained by ultrafiltration. Korean J. Food Sci. Technol., 48(3), 268-274 (2016). https://doi.org/10.9721/KJFST.2016.48.3.268
  6. Abo, T., Kawamura, T., Watanabe, H., Immunologic states of autoimmune diseases. Immunol. Res., 33(1), 23-34 (2005). https://doi.org/10.1385/IR:33:1:023
  7. Hibbs, J.B. Jr., Taintor, R.R., Vavrin, Z., Rachlin, E.M., Nitric oxide: A cytotoxic activated macrophage effector molecule. Biochem. Bioph. Res. Co., 157, 87-94 (1998). https://doi.org/10.1016/S0006-291X(88)80015-9
  8. Nathan, C.F., Secretory products of macrophages. J. Clin. Invest., 79, 319-326 (1987). https://doi.org/10.1172/JCI112815
  9. Johnston, R.B. Jr., Keele, B.B. Jr., Misra, H.P., Lehmeyer, J.E., Webb, L.S., Baehner, R.L., RaJagopalan, K.V., The role of superoxide anion generation in phagocytic bactericidal activity. Studies with normal and chronic granulomatous disease leukocytes. J. Clin. Invest., 55(6), 1357-1372 (1975). https://doi.org/10.1172/JCI108055
  10. Langermans, J.A., Van der Hulst, M.E., Nibbering, P.H., Hiemstra, P.S., Fransen, L., Van Furth, R., IFN-gammainduced L-arginine-dependent toxoplasmastatic activity in murine peritoneal macrophages is mediated by endogenous tumor necrosis factor-alpha. J. Immunol., 148(2), 568-574 (1992).
  11. Henry, Y., Ducrocq, C., Drapier, J.C., Servent, D., Pellat, .C., Guissani, A., Nitric oxide, a biological effector. Electron paramagnetic resonance detection of nitrosyl-iron-protein complexes in whole cells. Eur. Biophys. J., 20(1), 1-15 (1991). https://doi.org/10.1007/BF00183275
  12. Jin, M., Chung, K.S., Enhancement of immune responses by a water soluble proteoglycan, lepidan from Lentinus lepideus. Yakhak Hoeji, 43(5), 635-641 (1999).
  13. Herberman, R.B., Ortaldo, J.R., Natural killer cell: Their roles in defenses against disease. Science, 214, 24-30 (1981). https://doi.org/10.1126/science.7025208
  14. Boisvert, C.A., The pelvic fin and girdle of Panderichthys and the origin of tetrapod locomotion. Nature, 438, 1145-1147 (2005). https://doi.org/10.1038/nature04119
  15. Knothe Tate, M.L., Adamson, J.R., Tami, A.E., Bauer, T.W., The osteocyte. Int. J. Biochem. Cell Biol., 36(1), 1-8 (2004). https://doi.org/10.1016/S1357-2725(03)00241-3
  16. Teitelbaum, S.L., Bone resorption by osteoclasts. Science, 289, 1504-1508 (2000). https://doi.org/10.1126/science.289.5484.1504
  17. Ducy, P., Zhang, R., Geoffroy, V., Ridall, A.L., Karsenty, G., Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation. Cell, 89(5), 747-754 (1997). https://doi.org/10.1016/S0092-8674(00)80257-3
  18. Celeste, A.J, Iannazzi, J.A., Taylor, R.C., Hewick, R.M., Rosen, V., Wang, E.A., Wozney, J.M., Identification of transforming growth factor beta family members present in boneinductive protein purified from bovine bone. Proc. Natl. Acad. Sci. USA, 87, 9843-9847 (1990). https://doi.org/10.1073/pnas.87.24.9843
  19. Suda, T., Takahashi, N., Martin, T.J., Modulation of osteoclast differentiation. Endocr. Rev., 13(1), 66-80 (1992).
  20. Mitsunaga, T., Doi, T., Kondo, Y., Abe, I., Color development of proanthocyanidins in vanillin-hydrochloric acid reaction. J. Wood Sci., 44, 125-130 (1998). https://doi.org/10.1007/BF00526257
  21. Zhang, L., Cheng, Y.X., Liu, A.L., Wang, H.D., Wang, Y.L., Du, G.H., Antioxidant, anti-inflammatory and anti-influenza properties of components from Chaenomeles speciosa. Molecules, 15(11), 8507-8517 (2010). https://doi.org/10.3390/molecules15118507
  22. Choung, M.G., Lim, J.D., Antioxidant, anticancer and immune activation of anthocyanin fraction from Rubus coreanus Miquel fruits (Bokbunja). Korean J. Medicinal Crop. Sci., 20(4), 259-269 (2012). https://doi.org/10.7783/KJMCS.2012.20.4.259
  23. Kim, H.S., Kang, J.S., Preparation and characteristics of bread by medicinal herb composites with immunostimulation activity. J. Korean Soc. Food Sci. Nutr., 37, 109-116 (2008). https://doi.org/10.3746/jkfn.2008.37.1.109
  24. Cho, C.W., Han, C.J., Rhee, Y.K., Lee, Y.C., Shin, K.S., Shin, J.S., Lee, K.T., Hong, H.D., Cheonggukjang polysaccharides enhance immune activities and prevent cyclophosphamide- induced immunosuppression. Int. J. Biol. Macromol., 72, 519-525 (2015). https://doi.org/10.1016/j.ijbiomac.2014.09.010
  25. Yu, A.R., Park, H.Y., Kim, Y.S., Ha, S.K., Hong, H.D., Choi, H.D., Immuno-enhancing effect of seed extracts on a RAW 264.7 macrophage cell line. J. Korean Soc. Food Sci. Nutr., 41, 1671-1676 (2012). https://doi.org/10.3746/jkfn.2012.41.12.1671
  26. Yoon, W.J., Composition for immunity stimulatory activity using a fruit extract of Litsea japonica. Korea Patent NO. 1020170084331 (2017).
  27. Cha, J.H., Kim, Y.S., Lee, E.M., Effects of Prunellae spica water extract on immune response in macrophage cells. J. Korean Obstet. Gynecol., 23, 91-100 (2010). https://doi.org/10.15204/JKOBGY.2010.23.3.091
  28. Aloia, J.F., Cohr, S.H., Vaswani, A., Yeh, J.K., Yuen, K., Ellis, K., Risk factors for postmenopausal osteoporosis. Am. J. Med., 78, 95-100 (1985).