Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Simultaneous quantification of six nonpolar ginsenosides in white ginseng by reverse-phase high-performance liquid chromatography coupled with integrated pulsed amperometric detection

  • Song, Hyeyoung (Department of Oriental Pharmaceutical Sciences, Graduate School, Kyung Hee University) ;
  • Song, Kyung-Won (Department of Oral medicine, School of Dentistry, Dankook University) ;
  • Hong, Seon-Pyo (Department of Oriental Pharmaceutical Sciences, Graduate School, Kyung Hee University)
  • Received : 2019.01.03
  • Accepted : 2019.07.22
  • Published : 2020.07.15
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Abstract

Background: White ginseng consists of the roots and rhizomes of the Panax species, and red ginseng is made by steaming and drying white ginseng. While red ginseng has both polar and nonpolar ginsenosides, previous studies showed white ginseng to have only polar ginsenosides. Because nonpolar ginsenosides are formed through the manufacture of red ginseng from white ginseng, researchers have generally thought that nonpolar ginsenosides do not exist in white ginseng. Methods: We developed a simultaneous quantitative method for six nonpolar ginsenosides in white ginseng using reverse-phase high-performance liquid chromatography coupled with integrated pulsed amperometric detection. The nonpolar ginsenosides of white ginseng were extracted for 4 h under reflux with 50% methanol. Results: Using the gradient elution system, all target components were completely separated within 50 min. Nonpolar ginsenosides were determined in the rhizome head (RH), main root (MR), lateral root, and hairy root (HR) of 6-year-old white ginseng samples obtained from several regions (Geumsan, Punggi, and Kanghwa). The total content in the HR of white ginseng was 37.8-56.8% of that in the HR of red ginseng. The total content in the MR of white ginseng was 5.9-24.3% of that in the MR of red ginseng. In addition, the total content in the RH of white ginseng was 28.5-35.8% of that in the HR of red ginseng Conclusion: It was confirmed that nonpolar ginsenosides known to be specific components of red ginseng were present at substantial concentrations in the HR or RH of white ginseng.

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References

  1. Kitagawa I, Yoshikawa M, Yoshihara M, Hayashi T, Taniyama T. Chemical studies of crude drugs (1). Constituents of Ginseng radix rubra. Yakugaku Zasshi 1983;103:612-22. https://doi.org/10.1248/yakushi1947.103.6_612
  2. Kitagawa I, Taniyama T, Shibuya H, Noda T, Yoshikawa M. Chemical studies on crude drug processing. V. On the constituents of ginseng radix rubra (2): comparison of the constituents of white ginseng and red ginseng prepared from the same Panax ginseng root. Yakugaku Zasshi 1987;107:495-505. https://doi.org/10.1248/yakushi1947.107.7_495
  3. Chang Y, Wang SJ. Ginsenoside $Rg_{1}$ and $Rb_{1}$ enhance glutamate exocytosis from rat cortical nerve terminals by affecting vesicle mobilization through the activation of protein kinase C. Eur J Pharmacol 2008;590:74-9. https://doi.org/10.1016/j.ejphar.2008.05.032
  4. Ye R, Li N, Han J, Kong X, Cao R, Rao Z, Zhao G. Neuroprotective effects of ginsenoside Rd against oxygen-glucose deprivation in cultured hippocampal neurons. Neurosci Res 2009;64:306-10. https://doi.org/10.1016/j.neures.2009.03.016
  5. Tian J, Fu F, Geng M, Jiang Y, Yang J, Jiang W, Wang C, Liu K. Neuroprotective effect of 20(S)-ginsenoside $Rg_{3}$ on cerebral ischemia in rats. Neurosci Lett 2005;374:92-7. https://doi.org/10.1016/j.neulet.2004.10.030
  6. Li N, Liu B, Dluzen DE, Jin Y. Protective effects of ginsenoside $Rg_{2}$ against glutamate-induced neurotoxicity in PC12 cells. J Ethnopharmacol 2007;111:458-63. https://doi.org/10.1016/j.jep.2006.12.015
  7. Lee MS, Hwang JT, Kim SH, Yoon S, Kim MS, Yang HJ, Kwon DY. Ginsenoside Rc, an active component of Panax ginseng, stimulates glucose uptake in C2C12 myotubes through an AMPK-dependent mechanism. J Ethnopharmacol 2010;127:771-6. https://doi.org/10.1016/j.jep.2009.11.022
  8. Xie JT, Mehendale SR, Li X, Quigg R, Wang X, Wang CZ, Wu JA, Aung HH, Rue PA, Bell GI, et al. Anti-diabetic effect of ginsenoside Re in ob/ob mice. Biochim Biophys Acta 2005;1740:319-25. https://doi.org/10.1016/j.bbadis.2004.10.010
  9. Kang KS, Kang BC, Lee BJ, Che JH, Li GX, Trosko JE, Lee YS. Preventive effect of epicatechin and ginsenoside $Rb_{2}$ on the inhibition of gap junctional intercellular communication by TPA and $H_{2}O_{2}$. Cancer Lett 2000;152:97-106. https://doi.org/10.1016/S0304-3835(99)00438-3
  10. Yue PY, Wong DY, Wu PK, Leung PY, Mak NK, Yeung HW, Liu L, Cai Z, Jiang ZH, Fan TP, et al. The angiosuppressive effects of 20(R)-ginsenoside $Rg_{3}$. Biochem Pharmacol 2006;72:437-45. https://doi.org/10.1016/j.bcp.2006.04.034
  11. Luo X, Wang CZ, Chen J, Song WX, Luo J, Tang N, He BC, Kang Q, Wang Y, Du W, et al. Characterization of gene expression regulated by American ginseng and ginsenoside $Rg_{3}$ in human colorectal cancer cells. Int J Oncol 2008;32:975-83.
  12. Lee KY, Lee YH, Kim SI, Park JH, Lee SK. Ginsenoside-$Rg_{5}$ suppresses cyclin Edependent protein kinase activity via up-regulating p21Cip/WAF1 and downregulating cyclin E in SK-HEP-1 cells. Anticancer Res 1997;17:1067-72.
  13. Ota T, Maeda M, Odashima S, Ninomiya-Tsuji J, Tatsuka M. $G_{1}$ phase-specific suppression of the Cdk2 activity by ginsenoside $Rh_{2}$ in cultured murine cells. Life Sci 1997;60:PL39-44. https://doi.org/10.1016/S0024-3205(96)00608-X
  14. Kang KS, Kim HY, Yamabe N, Park JH, Yokozawa T. Preventive effect of 20 (S)-ginsenoside $Rg_{3}$ against lipopolysaccharide-induced hepatic and renal injury in rats. Free Radic Res 2007;41:1181-8. https://doi.org/10.1080/10715760701581740
  15. Park YC, Lee CH, Kang HS, Kim KW, Chung HT, Kim HD. Ginsenoside-$Rh_{1}$ and $Rh_{2}$ inhibit the induction of nitric oxide synthesis in murine peritoneal macrophages. Biochem Mol Biol Int 1996;40:751-7.
  16. Kang KS, Kim HY, Yamabe N, Yokozawa T. Stereospecificity in hydroxyl radical scavenging activities of four ginsenosides produced by heat processing. Bioorg Med Chem Lett 2006;16:5028-31. https://doi.org/10.1016/j.bmcl.2006.07.071
  17. Kim ND, Kim EM, Kang KW, Cho MK, Choi SY, Kim SG. Ginsenoside $Rg_{3}$ inhibits phenylephrine-induced vascular contraction through induction of nitric oxide synthase. Br J Pharmacol 2003;140:661-70. https://doi.org/10.1038/sj.bjp.0705490
  18. Lee HU, Bae EA, Han MJ, Kim DH. Hepatoprotective effect of 20(S)-Ginsenosides $Rg_{3}$ and its metabolite 20(S)-Ginsenoside Rh2 on tert-butyl hydroperoxide-induced liver injury. Biol Pharm Bull 2005;28. 1992-4. https://doi.org/10.1248/bpb.28.1992
  19. Kim WY, Kim JM, Han SB, Lee SK, Kim ND, Park MK, Kim CK, Park JH. Steaming of ginseng at high temperature enhances biological activity. J Natural Products 2000;63:1702-4. https://doi.org/10.1021/np990152b
  20. Kang KS, Kim HY, Pyo JS, Yokozawa T. Increase in the free radical scavenging activity of ginseng by heat-processing. Biol Pharm Bull 2006;29:750-4. https://doi.org/10.1248/bpb.29.750
  21. Jin F, Yu H, Fu Y, An DS, Im WT, Lee ST, Silva JAT. Biotransformation of ginsenosides. Int J Biomed Pharmaceut Sci 2009;6:33-44.
  22. Kim SN, Ha YW, Shin H, Son SH, Wu SJ, Kim YS. Simultaneous quantification of 14 ginsenosides in Panax ginseng C.A. Meyer (Korean red ginseng) by HPLCELSD and its application to quality control. J Pharm Biomed Anal 2007;45.164-70. https://doi.org/10.1016/j.jpba.2007.05.001
  23. Park HW, In G, Han ST, Lee MW, Kim SY, Kim KT, Cho BG, Han GH, Chang IM. Simultaneous determination of 30 ginsenosides in Panax ginseng preparations using ultra performance liquid chromatography. J Ginseng Res 2013;37:457-67. https://doi.org/10.5142/jgr.2013.37.457
  24. Leu L, Huang J, Hu X, Li K, Sun CH. Simultaneous determination of ginsenoside(G-Re, G-$Rg_{1}$, G-$Rg_{2}$, G-$F_{1}$, G-$Rh_{1}$) and protopanaxatriol in human plasma and urine by LC-MS/MS and its application in a pharmacokinetics study of G-Re in volunteers. J Chromatogr B 2011;879:2011-7. https://doi.org/10.1016/j.jchromb.2011.05.018
  25. Xie G, Plumb R, Su M, Xu Z, Zhao A, Qiu M, Long X, Liu Z, Jia W. Ultra-performance LC/TOF MS analysis of medicinal Panax herbs for metabolomic research. J Sep Sci 2008;31. 1015-26. https://doi.org/10.1002/jssc.200700650
  26. Lee SI, Kwon HJ, Lee YM, Lee JH, Hong SP. Simultaneous analysis method for polar and non-polar ginsenosides in red ginseng by reversed-phase HPLCPAD. J Pharm Biomed Anal 2012;60:80-5. https://doi.org/10.1016/j.jpba.2011.08.030
  27. Kwon HJ, Jeong JS, Sim HJ, Hong SP. Sensitive high-performance liquid chromatography method of non-polar ginsenosides by alkaline-enhanced pulsed amperometric detection. J Chromatogr A 2009;1216:4445-50. https://doi.org/10.1016/j.chroma.2009.03.031
  28. Johnson DC, LaCourse WR. Liquid chromatography with pulsed electrochemical detection at Gold and platinum electrodes. Anal Chem 1990;62.589A-97A. https://doi.org/10.1021/ac00209a715
  29. Mellado-Mojica E, Lopez MG. Identification, classification, and discrimination of agave syrups from natural sweeteners by infrared spectroscopy and HPAEC-PAD. Food Chem 2015;167:349-57. https://doi.org/10.1016/j.foodchem.2014.06.111
  30. Draisci R, Cavalli S, Lucentini L, Stacchini A. Ion exchange separation and pulsed amperometric detection for determination of biogenic amines in fish products. Chromatographia 1993;35:584-90. https://doi.org/10.1007/BF02267921
  31. Draisci R, Giannetti L, Boria P, Lucentini L, Palleschi L, Cavalli S. Improved ion chromatographyeintegrated pulsed amperometric detection method for the evaluation of biogenic amines in food of vegetable or animal origin and in fermented foods. J Chromatogr A 1998;798:109-16. https://doi.org/10.1016/S0021-9673(97)01198-9
  32. Wang L, Weller CL. Recent advances in extraction of nutraceuticals from plants. Trends Food Sci Technol 2006:300-12.
  33. Kim SJ, Murthy HM, Hahn EJ, Lee HL, Paek KY. Parameters affecting the extraction of ginsenosides from the adventitious roots of ginseng. Sep Purif Technol 2007;56:401-6. https://doi.org/10.1016/j.seppur.2007.06.014
  34. Kwon HJ, Jeong JS, Lee YM, Hong SP. A reversed-phase high-performance liquid chromatography method with pulsed amperometric detection for the determination of glycosides. J Chromatogr A 2008;1185:251-7. https://doi.org/10.1016/j.chroma.2008.01.052

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