Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Morpho-molecular characterization of diatom Skeletonema pseudocostatum(Thalassiosirales, Bacillariophyta) from the Korean coast

규조류 Skeletonema pseudocostatum Medlin (Thalassiosirales, Bacillariohyta)의 형태적 특징과 분자계통학적 위치

  • Han, Kyong Ha (Library of Marine Samples, Korea Institute of Ocean Science and Technology) ;
  • Li, Zhun (Biological Resource Center, Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Joon Sang (Marine Biotechnology Research Center, Korea Institute of Ocean Science & Technology) ;
  • Youn, Joo Yeon (Library of Marine Samples, Korea Institute of Ocean Science and Technology) ;
  • Kim, Hyun Jung (Library of Marine Samples, Korea Institute of Ocean Science and Technology) ;
  • Kwak, Kyeong Yoon (Library of Marine Samples, Korea Institute of Ocean Science and Technology) ;
  • Oh, Seok Jin (Department of Oceanography, Pukyong University) ;
  • Shin, Hyeon Ho (Library of Marine Samples, Korea Institute of Ocean Science and Technology)
  • 한경하 (한국해양과학기술원 해양시료도서관) ;
  • 이준 (한국생명공학연구원 생물자원센터) ;
  • 박준상 (한국해양과학기술원 생명공학연구센터) ;
  • 윤주연 (한국해양과학기술원 해양시료도서관) ;
  • 김현정 (한국해양과학기술원 해양시료도서관) ;
  • 곽경윤 (한국해양과학기술원 해양시료도서관) ;
  • 오석진 (부경대학교 해양학과) ;
  • 신현호 (한국해양과학기술원 해양시료도서관)
  • Received : 2019.11.05
  • Accepted : 2020.01.15
  • Published : 2020.03.31
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Abstract

Morphology of a strain of unspecified Skeletonema species established from Korean coast was examined by light, fluorescence and scanning electron microscopy, and SSU(small subunit) and LSU(large subunit) rDNA of the strain were also sequenced. The specimen was characterized by solitary or short chains, and each cell contained 1-2 chloroplasts. The valve face was slightly convex, and the terminal fultoportula processes (TFPPs) were open and showed narrow distal ends that could be truncated or spiny with claw-like protrusions. The basal part of the TFPPs was tubular and oblique to the cell axis. The intercalary fultoportula processes (IFPPs) were also narrow, completely open, and joined in a 1 : 1 junction. Occasionally, one IFPP was connected with two opposing IFPPs. The morphological features of the specimen were identical to those of Skeletonema pseudocostatum. Molecular phylogeny based on SSU rDNA revealed that the Korean strain is nested within a clade comprising S. pseudocostatum and S. tropicum. However, based on D1-D2 LSU rDNA sequences, a clade including S. pseudocostatum and a Korean strain was separated from the S. tropicum clade. This indicates that the Korean strain can be identified as S. pseudocostatum. This species represents the first record from Korean coastal waters.

Skeletonema pseudocostatum의 세포는 규산질 성분의 돌기에 의한 사슬 형태로, 길이는 6~17.3 ㎛였고, 엽록체는 세포 당 1~2개를 포함하고 있었다. 주사전자현미경 관찰결과 Skeletonema 종을 구분할 수 있는 가장자리 받침돌기끝(terminal fultoportula process)은 끝이 갈라지거나, 갈고리 모양이었고, 길이가 1.67±0.5 ㎛이고, 개각의 가장자리에 위치해 있으며, 개수는 8.10±1.1개로 개각의 크기에 따라 다양하게 나타났다. 말단세포 입술돌기(terminal rimoportula process)는 두꺼운 원통형의 나팔관 모양으로, 개각의 중앙 근처에 위치하였고, 길이는 1.1±0.6 ㎛였으며, 개수는 1개였다. 연결세포 받침돌기(intercalary fultoportula process)는 대부분 1 : 1 결합으로 서로 맞물려 있는 형태였고, 1 : 2 결합도 종종 발견되었다. 계통분석 결과는 형태적 특징이 유사한 종 간의 유전학적 거리가 가깝다는 것을 나타냈고, 지리적 기원이 다른 동일 종의 경우, 유전적 차이를 보이지 않았다. 이는 S. pseudocostatum은 지리적 위치와 상관없이 유전적 차이가 나타나지 않는 단일계통(monophyly)이라는 것을 의미한다.

Keywords

References

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