Journal of Korean Medicine Rehabilitation (한방재활의학과학회지)

The society of Korean Medicine Rehabilitation (한방재활의학과학회)
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The Preclinical Study of Hyeolbuchugeo-tang (Xuefuzhuyu-tang) on Bone Healing in Rats with Rib Fracture

골절 유발 Rat에 대한 혈부축어탕(血府逐瘀湯)의 전임상 연구

  • Huh, Gun (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Daejeon University) ;
  • Oh, Min-Seok (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Daejeon University)
  • 허건 (대전대학교 한의과대학 한방재활의학교실) ;
  • 오민석 (대전대학교 한의과대학 한방재활의학교실)
  • Received : 2020.06.17
  • Accepted : 2020.06.23
  • Published : 2020.07.31
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Abstract

Objectives The purpose of this study is to evaluate the healing effect of Hyeolbuchugeo-tang (HC) in rats with rib fracture. Methods Rats were randomly divided into 5 groups (naive, control, positive control, HC-L and HC-H). All groups except naive group were subjected to bone fracture of rib. Naive group received no treatment at all. Control group was fed with phosphate buffered saline. Positive control group was orally medicated with tramadol. Experimental group was orally medicated with HC extract (50 mg/kg for low concentration [HC-L], 100 mg/kg for high concentration [HC-H]). X-ray and micro-computed tomography (micro-CT) were conducted to assess the effect of HC. We analysed the level of 2) transforming growth factor-β1 (TGF-β1), Ki67, alkaline phosphatase (ALP), receptor activator of nuclear factor kappa-β, runt-related transcription factor 2 (Runx2) and tartrate resistant acid phosphatase (TRAP) on 7 and 14 days after fracture. ALP, alanine aminotransferase, aspartate aminotransferase, blood urea nitrogen, creatinine was measured for safety assessment. Results X-ray and micro-CT, showed HC enhance bone repair process. Callus formation was increased in experimental group at 7 days after fracture, but decreased at 14 days after fracture. 7 days after fracture, the level of TGF-β1 in experimental group was decreased. The level of Ki67, Runx2 in HC-H, TRAP in HC-L was increased. 14 days after fracture, the level of Ki67 in HC-L and HC-H was decreased. The level of ALP, Runx2, BUN in HC-L, TRAP in HC-L and HC-H was increased. Conclusions Taken together the results, HC promoted healing of bone fracture. In conclusion, HC has a potential to promote healing of bone fracture.

Keywords

References

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