Journal of Korean Medicine Rehabilitation (한방재활의학과학회지)

The society of Korean Medicine Rehabilitation (한방재활의학과학회)
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Anti-oxidant and Anti-inflammatory Effects of Chulbu-tang

출부탕(朮附湯) 추출물의 항산화 및 항염증에 대한 효과

  • Hyeong, Kyun (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Daegu Haany University) ;
  • Won, Je-Hoon (Department of Rehabilitation Medicine of Korean Medicine, Dongguk University Gyeongju Oriental Medicine Clinic) ;
  • Woo, Chang-Hoon (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine, Daegu Haany University)
  • 형균 (대구한의대학교 한의과대학 한방재활의학과교실) ;
  • 원제훈 (동국대학교 경주한의원 한방재활의학과) ;
  • 우창훈 (대구한의대학교 한의과대학 한방재활의학과교실)
  • Received : 2020.06.13
  • Accepted : 2020.06.26
  • Published : 2020.07.31
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Abstract

Objectives Even though the various alternative herbal medicine has applied for osteoarthritis (OA) treatment, its scientific proof remains uncertain. The aim of the present study evaluates the effects of Chulbu-tang on inflammatory responses in a monosodium iodoacetate (MIA)-induced osteoarthritis rat model. Methods OA rat model was established by MIA injection in intra-joint of rats. 7 days after, OA rats except OA control rats were administrated Chulbu-tang (100 or 200 mg/kg) or Indomathacin (5 mg/kg) once a day for 14 days. The weight-bearing ability of hind paws were measured when group isolation 0, 7, and 14 days. Western blotting was performed to examine the knockdown/overexpressing efficiency of Chulbu-tang. In addition, cartilage destruction was measured histologically. Results Chulbu-tang treatment significantly reduced the protein expressions of inflammatory mediators such as inducible nitric oxide synthase and cyclooxygenase 2, and inhibited inflammatory cytokines including tumor necrosis factor alpha, interleukin (IL)-1β, and IL-6 through nuclear factor-kappa B (NF-κB) inactivation. Moreover, anti-oxidant enzymes such as superoxide dismutase and glutathione peroxidase-1/2 through nuclear factor-erythroid 2-related factor 2 (Nrf2) pathway significantly increased. Our findings indicate that Chulbu-tang has the potential therapeutic effect on OA through inhibiting the inflammatory responses via inactivating NF-κB signaling pathway. In addition, upregulation of Nrf2 led to anti-oxidant effects. Conclusions Taken together, Chulbu-tang is believed to have antioxidant, anti-inflammatory effects, and cartilage protection for arthritis-causing rats.

Keywords

References

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