Korean Journal of Ecology and Environment (생태와환경)

The Korean Society of Limnology (한국수생태학회)
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Molecular Identification of Pseudanabaena Strains and Analysis of 2-MIB Production Potential in the North Han River System

북한강 수역에 분포하는 Pseudanabaena 균주의 동정 및 2-MIB 생산 잠재성 분석

  • Kim, Keonhee (Department of Environment and Health Science, Sanghuh College of Life Sciences, Konkuk University) ;
  • Lee, Sejin (Water Quality & Environment Center, Water Resources Management Dept, Hangang River Regional Division, K-water) ;
  • Seo, Kyunghwa (Water Quality & Environment Center, Water Resources Management Dept, Hangang River Regional Division, K-water) ;
  • Hwang, Soon-Jin (Department of Environment and Health Science, Sanghuh College of Life Sciences, Konkuk University)
  • 김건희 (건국대학교 상허생명과학대학 환경보건과학과) ;
  • 이세진 (K-water 한강유역본부 한강물관리처 물환경센터) ;
  • 서경화 (K-water 한강유역본부 한강물관리처 물환경센터) ;
  • 황순진 (건국대학교 상허생명과학대학 환경보건과학과)
  • Received : 2020.11.12
  • Accepted : 2020.12.12
  • Published : 2020.12.31
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Abstract

Identification of the target species of 2-MIB (2-methyllisoborneol) production is crucial in the management of off-flavor problem in the freshwater system. This study was conducted to identify 2-MIB-producing Pseudanabaena strains occurring in the North Han River system using molecular genetic method. Eleven phenotypes of Pseudanabaena were isolated from several mainstream sites of the North Han River, including Sambong-ri, Joam-myun, and Lake Uiam areas. Despite of morphological similarity of the strains, the phylogenetic analysis using 16S rDNA classified them into different species with low genetic similarity (40~55%). Isolated Pseudanabaena strains were converged to four species; Pseudanabaena cinerea, P. yagii, P. mucicola, and P. redekei. Among them, the 2-MIB synthesizing gene (mibC) was detected in P. cinerea, P. yagii, and P. redekei. However, actual 2-MIB production was detected only in P. cinerea and P. redekei based on gas chromatography analysis. This study is the first report of the molecular identification of Pseudanabaena strains and their 2-MIB production potential in Korea. The results of this study provides an evidence of species diversity of Pseudanabaena occurring in the North Han River.

이취미 물질인 2-MIB를 합성하는 원인종에 대한 정보는 담수생태계에서 이와 관련된 환경 및 경제적 문제를 해결하는 데 필수적이다. 본 연구는 북한강 수계에서 출현하는 Pseudanabaena strain을 분리·배양하고, 16S rDNA 염기서열을 이용하여 종 수준의 동정과 2-MIB 합성 유전자 탐색을 통해 이취미 물질 발생 잠재성을 분석하였다. 북한강 본류 지역(삼봉리, 조암면, 의암호 지역)에서 분리한 Pseudanabaena strain은 총 11개로서 NHUA201911과 NHPD201909 strain을 제외하고 단일세포의 크기는 서로 유사하였다. 그러나 16S rDNA 계통분석을 통한 유전자 염기서열의 유연관계를 분석한 결과, 분리된 strain들은 총 5개 종(P. cinerea, P. yagii, P. mucicola, P. galeata, P. redekei)으로 분류되었다(40~55% 유사도). 2-MIB를 합성하는 mibC 유전자는 P. cinerea 07 strain (NHUA202007-07)와 P. yagii (NHUA202007-08), P. redekei (NHUA201911)에서만 발견되었으며, 가스크로마토그래피 분석에 따라 실질적인 2-MIB 합성은 P. cinerea와 P. redekei 종에서 확인되었다. 본 연구결과는 분자생물학적 수준에서 북한강 수역에서 발생하는 Pseudanabaena속 남조류의 다양도에 대한 증거를 제공하는 연구로서 북한강 수계에서 2-MIB 생산 원인종에 대한 중요한 정보를 제공한다.

Keywords

Acknowledgement

본 연구는 2020년 한강수계관리위원회 환경기초조사사업(북한강수계 맛냄새물질 발생원인 조사 및 관리대책수립)의 지원을 받아 수행되었습니다.

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