Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Antioxidant components and antioxidant activities of mixtures with Sasa quelpaertensis Nakai and Ficus erecta var. sieboldii

좁은잎천선과 및 조릿대 혼합 추출물의 항산화 성분과 항산화 활성

  • Kwon, Hee-Yeon (Department Food Science and Biotechnology, Kangwon National University) ;
  • Choi, Sun-Il (Department Food Science and Biotechnology, Kangwon National University) ;
  • Han, Xionggao (Department Food Science and Biotechnology, Kangwon National University) ;
  • Men, Xiao (Department Food Science and Biotechnology, Kangwon National University) ;
  • Jang, Gill-Woong (Department Food Science and Biotechnology, Kangwon National University) ;
  • Choi, Ye-Eun (Department Food Science and Biotechnology, Kangwon National University) ;
  • Kang, Jun-Chul (Haram Central Research Institute) ;
  • Cho, Ju-Hyun (Haram Central Research Institute) ;
  • Lee, Ok-Hwan (Department Food Science and Biotechnology, Kangwon National University)
  • 권희연 (강원대학교 바이오산업공학부 식품생명공학전공) ;
  • 최선일 (강원대학교 바이오산업공학부 식품생명공학전공) ;
  • 한웅호 (강원대학교 바이오산업공학부 식품생명공학전공) ;
  • 문효 (강원대학교 바이오산업공학부 식품생명공학전공) ;
  • 장길웅 (강원대학교 바이오산업공학부 식품생명공학전공) ;
  • 최예은 (강원대학교 바이오산업공학부 식품생명공학전공) ;
  • 강준철 (하람 중앙연구소) ;
  • 조주현 (하람 중앙연구소) ;
  • 이옥환 (강원대학교 바이오산업공학부 식품생명공학전공)
  • Received : 2020.06.19
  • Accepted : 2020.07.23
  • Published : 2020.08.31
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Abstract

The purpose of this study was to evaluate the antioxidant components and activities of HR02/04(8:2)-W, a mixture of S. quelpaertensis Nakai and F. erecta var. sieboldii. We investigated the p-coumaric acid, total flavonoid, and total phenol contents. To evaluate the antioxidant efficacy, we measured the 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzthiazoline6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity, FRAP activity, reducing power, and ORAC value. We observed the protective effect of hydrogen peroxide against cell damage in human dermal fibroblasts. As a result of the experiment, the p-coumaric acid, total flavonoid, and total phenol contents were 75.62±1.56 mg/100 g, 21.57±0.84 mg rutin equivalent (RE)/g, and 21.25±1.31 mg gallic acid equivalent (GAE)/g, respectively. In the experiments on antioxidant activity, HR02/04(8:2)-W was found to have significantly increased antioxidant activity. In the human dermal fibroblasts, the HR02/04(8:2)-W treated groups could effectively protect cells against oxidative damage. In this study, we confirmed that HR02/04(8:2)-W is a material with effective physiological antioxidant activity.

본 연구에서는 HR02/04(8:2)-W에 대한 항산화 성분 및 생리활성을 평가하기 위하여, p-coumaric acid 함량, 총 플라보노이드 및 총 페놀 함량, DPPH, ABTS 라디칼 소거능, FRAP 활성, reducing power, ORAC value를 측정하였다. 또한, 피부 섬유아세포에서 hydrogen peroxide에 의하여 산화적 스트레스가 유도된 세포 모델에서의 HR02/04(8:2)-W의 산화 손상 보호효과를 측정하기 위하여 XTT assay 및 H2-DCFDA assay를 진행하였다. HPLC를 이용한 p-coumaric acid 함량 분석 결과, HR02/04(8:2)-W 내의 p-coumaric acid는 75.62±1.56 mg/100 g 함유되어 있는 것으로 나타났으며, 총 플라보노이드 함량 및 총 페놀 함량은 각각 21.57±0.84 mg RE/g, 21.25±1.31 mg GAE/g으로 측정되었다. DPPH 및 ABTS 라디칼 소거활성 시험에서 HR02/04(8:2)-W의 농도 유의적으로 소거활성이 증가하는 것을 확인하였으며, FRAP 활성 및 reducing power 측정에서도 우수한 항산화 활성을 보였다. ORAC assay 결과 control 보다 5배 이상 높은 수치를 보이며 효과적으로 라디칼 저해 활성을 보였다. 항산화 실험에서의 뛰어난 항산화 활성은 HR02/04(8:2)-W에 함유된 페놀류들에 의한 것으로 판단된다. 피부 섬유아세포를 이용한 실험에서, HR02/04(8:2)-W을 300 ㎍/mL의 농도까지 세포의 생존력에 영향을 미치지 않는 것으로 나타났으며, hydrogen peroxide 처리에 의하여 56%까지 감소되었던 세포 생존율을 최대 78%까지 증가시키며 효과적으로 세포를 보호했음을 확인하였다. H2-DCFDA 염색을 통하여 세포 내의 ROS의 양을 측정하였을 때, hydrogen peroxide처리에 의하여 control군 대비 137%까지 증가하였던 ROS를 최대 89% 까지 감소시킴으로써 세포 내 활성산소를 효과적으로 억제하였다. 이상의 결과를 종합하여 볼 때, HR02/04(8:2)-W이 뛰어난 항산화 생리활성 기능을 가지고, 세포 내 활성산소를 효과적으로 제거할 수 있는 소재임이 확인되었으며, 천연물 유래 기능성 식품원료로서의 활용 가능성이 매우 넓을 것으로 판단된다.

Keywords

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