Clinical and Experimental Reproductive Medicine

  • 제47권3호
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  • Pages.161-167
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  • 2020
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  • 2233-8233(pISSN)
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  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
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Cichorium intybus L. extract ameliorates testicular oxidative stress induced by lead acetate in male rats

  • 투고 : 2019.12.20
  • 심사 : 2020.04.06
  • 발행 : 2020.09.30
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초록

Objective: Oxidative stress has been suggested as a possible mechanism for the adverse effects of heavy metal toxicity on male reproduction. Cichorium intybus L. is used in Iranian folk medicine as a hepatoprotective agent as well as for its supposed fertility-enhancing properties. The present study was performed to investigate whether the ethanolic extract of C. intybus leaves could protect male rats against lead-induced testicular oxidative stress. Methods: In this experimental study, adult Wistar rats were treated with 0.1% lead acetate in drinking water alone or with 50, 100, or 200 mg/kg body weight of C. intybus extract via gavage once daily for 70 days. The weight of their reproductive organs, levels of serum hormones, histometric parameters of the seminiferous tubules, epidydimal sperm quality, and oxidative stress status were evaluated. Results: The testis weight, seminiferous tubule diameter, epididymal sperm count, serum testosterone level, and testicular levels of superoxide dismutase and glutathione peroxidase were significantly reduced (p< 0.05) in the lead-treated rats. Moreover, significantly (p< 0.05) higher levels of malondialdehyde were observed in the lead-exposed group compared to the control. However, the co-administration of C. intybus ethanolic extract in lead-treated rats was associated with a significant improvement in reproductive parameters. Conclusion: We conclude that C. intybus leaf extract has the potential to prevent lead-induced testicular toxicity and to suppress the adverse effects of lead on male reproductive health.

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참고문헌

  1. Jensen TK, Bonde JP, Joffe M. The influence of occupational exposure on male reproductive function. Occup Med (Lond) 2006;56:544-53. https://doi.org/10.1093/occmed/kql116
  2. Basnet P, Hansen SA, Olaussen IK, Hentemann MA, Acharya G. Changes in the semen quality among 5739 men seeking infertility treatment in Northern Norway over past 20 years (1993-2012). J Reprod Biotechnol Fertil 2016;5:1-7.
  3. Meeker JD, Rossano MG, Protas B, Diamond MP, Puscheck E, Daly D, et al. Cadmium, lead, and other metals in relation to semen quality: human evidence for molybdenum as a male reproductive toxicant. Environ Health Perspect 2008;116:1473-9. https://doi.org/10.1289/ehp.11490
  4. Telisman S, Colak B, Pizent A, Jurasovic J, Cvitkovic P. Reproductive toxicity of low-level lead exposure in men. Environ Res 2007;105:256-66. https://doi.org/10.1016/j.envres.2007.05.011
  5. Wyrobek AJ, Schrader SM, Perreault SD, Fenster L, Huszar G, Katz DF, et al. Assessment of reproductive disorders and birth defects in communities near hazardous chemical sites. III. Guidelines for field studies of male reproductive disorders. Reprod Toxicol 1997;11(2-3):243-59. https://doi.org/10.1016/S0890-6238(96)00108-6
  6. Kumar S. Occupational and environmental exposure to lead and reproductive health impairment: an overview. Indian J Occup Environ Med 2018;22:128-37.
  7. Eibensteiner L, Del Carpio Sanz A, Frumkin H, Gonzales C, Gonzales GF. Lead exposure and semen quality among traffic police in Arequipa, Peru. Int J Occup Environ Health 2005;11:161-6. https://doi.org/10.1179/oeh.2005.11.2.161
  8. Hernandez-Ochoa I, Garcia-Vargas G, Lopez-Carrillo L, Rubio-Andrade M, Moran-Martinez J, Cebrian ME, et al. Low lead environmental exposure alters semen quality and sperm chromatin condensation in northern Mexico. Reprod Toxicol 2005;20:221-8. https://doi.org/10.1016/j.reprotox.2005.01.007
  9. Bolin CM, Basha R, Cox D, Zawia NH, Maloney B, Lahiri DK, et al. Exposure to lead and the developmental origin of oxidative DNA damage in the aging brain. FASEB J 2006;20:788-90. https://doi.org/10.1096/fj.05-5091fje
  10. Marchlewicz M, Michalska T, Wiszniewska B. Detection of leadinduced oxidative stress in the rat epididymis by chemiluminescence. Chemosphere 2004;57:1553-62. https://doi.org/10.1016/j.chemosphere.2004.08.102
  11. Gurer H, Ercal N. Can antioxidants be beneficial in the treatment of lead poisoning? Free Radic Biol Med 2000;29:927-45. https://doi.org/10.1016/S0891-5849(00)00413-5
  12. de Lamirande E, Jiang H, Zini A, Kodama H, Gagnon C. Reactive oxygen species and sperm physiology. Rev Reprod 1997;2:48-54. https://doi.org/10.1530/revreprod/2.1.48
  13. Shalan MG, Mostafa MS, Hassouna MM, El-Nabi SE, El-Refaie A. Amelioration of lead toxicity on rat liver with Vitamin C and silymarin supplements. Toxicology 2005;206:1-15. https://doi.org/10.1016/j.tox.2004.07.006
  14. Ilaiyaraja N, Khanum F. Evaluation of antioxidant and toxicological properties of chicory leaves. IJPBA 2010;1:155-63.
  15. Mirhaydar H. Plants sciences: application of plants in prophylaxisand treatment of diseases. Tehran: Daftar Nashr Farhang Islami; 1993.
  16. Zargari A. Medicinal plants. Tehran: Tehran University Publication; 1996.
  17. Behnam Rasouli M, Hosseinzade H, Ali Akbarpour A. The effects of Soxhlet aqueous extract of Chicory leaves on the blood pH and cations level & the sex of newborns in rat. Daneshvar Med 2000;27:57-64.
  18. Dorostghoal M, Seyyednejad SM, Nejad MN. Beneficial effects of Cichorium intybus L. extract on oxidative status and reproductive parameters in male Wistar rats: An experimental study. Int J Reprod Biomed (Yazd) 2019;17:425-34.
  19. Das S, Vasudeva N, Sharma S. Cichorium intybus: A concise report on its ethnomedicinal, botanical, and phytopharmacological aspects. Drug Dev Ther 2016;7:1-12. https://doi.org/10.4103/2394-6555.180157
  20. Kim JH, Mun YJ, Woo WH, Jeon KS, An NH, Park JS. Effects of the ethanol extract of Cichorium intybus on the immunotoxicity by ethanol in mice. Int Immunopharmacol. 2002;2:733-44. https://doi.org/10.1016/S1567-5769(02)00008-5
  21. Hayes W. Principle and methods of toxicology. New York: Raven Press; 1989.
  22. El-Sayed YS, El-Neweshy MS. Impact of lead toxicity on male rat reproduction at hormonal and histopathological levels. Toxicol Environ Chem 2010;4:765-74. https://doi.org/10.1080/02772240902984453
  23. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8. https://doi.org/10.1016/0003-2697(79)90738-3
  24. Rubio J, Riqueros MI, Gasco M, Yucra S, Miranda S, Gonzales GF. Lepidium meyenii (Maca) reversed the lead acetate induced--damage on reproductive function in male rats. Food Chem Toxicol 2006;44:1114-22. https://doi.org/10.1016/j.fct.2006.01.007
  25. Michael B, Yano B, Sellers RS, Perry R, Morton D, Roome N, et al. Evaluation of organ weights for rodent and non-rodent toxicity studies: a review of regulatory guidelines and a survey of current practices. Toxicol Pathol 2007;35:742-50. https://doi.org/10.1080/01926230701595292
  26. Sainath SB, Meena R, Supriya Ch, Reddy KP, Reddy PS. Protective role of Centella asiatica on lead-induced oxidative stress and suppressed reproductive health in male rats. Environ Toxicol Pharmacol 2011;32:146-54. https://doi.org/10.1016/j.etap.2011.04.005
  27. Hamadouche NA, Sadi N, Kharoubi O, Slimani M, Aoues A. The protective effect of vitamin E against genotoxicity of lead acetate intraperitoneal administration in male rat. Arch Biol Sci 2013;65:1435-45. https://doi.org/10.2298/ABS1304435H
  28. Anjum MR, Madhu P, Reddy KP, Reddy PS. The protective effects of zinc in lead-induced testicular and epididymal toxicity in Wistar rats. Toxicol Ind Health 2017;33:265-76. https://doi.org/10.1177/0748233716637543
  29. Mruk DD, Cheng CY. Sertoli-Sertoli and Sertoli-germ cell interactions and their significance in germ cell movement in the seminiferous epithelium during spermatogenesis. Endocr Rev 2004;25:747-806. https://doi.org/10.1210/er.2003-0022
  30. Yoshida M, Kitani T, Takenaka A, Kudoh K, Katsuda SI, Taya K, et al. Lack of effects of oxolinic acid on spermatogenesis in young adult and aged Wistar rats. Food Chem Toxicol 2002;40:1815-25. https://doi.org/10.1016/S0278-6915(02)00168-0
  31. Chandra AK, Ghosh R, Chatterjee A, Sarkar M. Effects of vanadate on male rat reproductive tract histology, oxidative stress markers and androgenic enzyme activities. J Inorg Biochem 2007;101:944-56. https://doi.org/10.1016/j.jinorgbio.2007.03.003
  32. Uzunhisarcikli M, Kalender Y, Dirican K, Kalender S, Ogutcu A, Buyukkomurcu F. Acute, subacute and subchronic administration of methyl parathion-induced testicular damage in male rats and protective role of vitamins C and E. Pestic Biochem Physiol 2007;87:115-22. https://doi.org/10.1016/j.pestbp.2006.06.010
  33. Raji Y, Udoh US, Mewoyeka OO, Ononye FC, Bolarinwa AF. Implication of reproductive endocrine malfunction in male antifertility efficacy of Azadirachta indica extract in rats. Afr J Med Sci 2003;32:159-65.
  34. Dobrakowski M, Pawlas N, Kasperczyk A, Kozlowska A, Olewinska E, Machon-Grecka A, et al. Oxidative DNA damage and oxidative stress in lead-exposed workers. Hum Exp Toxicol 2017;36:744-54. https://doi.org/10.1177/0960327116665674
  35. Martinez-Haro M, Green AJ, Mateo R. Effects of lead exposure on oxidative stress biomarkers and plasma biochemistry in waterbirds in the field. Environ Res 2011;111:530-8. https://doi.org/10.1016/j.envres.2011.02.012
  36. Patrick L. Lead toxicity part II: the role of free radical damage and the use of antioxidants in the pathology and treatment of lead toxicity. Altern Med Rev 2006;11:114-27.
  37. Agarwal A, Virk G, Ong C, du Plessis SS. Effect of oxidative stress on male reproduction. World J Mens Health 2014;32:1-17. https://doi.org/10.5534/wjmh.2014.32.1.1
  38. Aitken RJ, Smith TB, Jobling MS, Baker MA, De Iuliis GN. Oxidative stress and male reproductive health. Asian J Androl 2014;16:31-8. https://doi.org/10.4103/1008-682x.122203
  39. Jurgonbski A, Milala J, Jusbkiewicz J, Zdunbczyk Z, Boguslaw Krol. Composition of chicory root, peel, seed and leaf ethanol extracts and biological properties of their non-inulin fractions. Food Technol Biotechnol 2011;49:40-7.
  40. Jamshidzadeh A, Khoshnood MJ, Dehghani Z, Niknahad H. Hepatoprotective activity of Cichorium intybus L. leaves extract against carbon tetrachloride induced toxicity. Iran J Pharm Res 2006;5:41-6.
  41. Gadgoli C, Mishra SH. Antihepatotoxic activity of Cichorium intybus. J Ethnopharmacol 1997;58:131-4. https://doi.org/10.1016/S0378-8741(97)00090-1
  42. Ahmed B, Al-Howiriny TA, Siddiqui AB. Antihepatotoxic activity of seeds of Cichorium intybus. J Ethnopharmacol 2003;87:237-40. https://doi.org/10.1016/S0378-8741(03)00145-4
  43. Rice-Evans C, Miller N, Paganga G. Antioxidant properties of phenolic compounds. Trends Plant Sci 1997;2:152-9. https://doi.org/10.1016/S1360-1385(97)01018-2
  44. Sakihama Y, Mano J, Sano S, Asada K, Yamasaki H. Reduction of phenoxyl radicals mediated by monodehydroascorbate reductase. Biochem Biophys Res Commun 2000;279:949-54. https://doi.org/10.1006/bbrc.2000.4053
  45. Blokhina O, Virolainen E, Fagerstedt KV. Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann Bot 2003;91 Spec No(2):179-94. https://doi.org/10.1093/aob/mcf118
  46. Van den Ende W, Valluru R. Sucrose, sucrosyl oligosaccharides, and oxidative stress: scavenging and salvaging? J Exp Bot 2009;60:9-18. https://doi.org/10.1093/jxb/ern297

피인용 문헌

  1. Environmental Factors-Induced Oxidative Stress: Hormonal and Molecular Pathway Disruptions in Hypogonadism and Erectile Dysfunction vol.10, pp.6, 2020, https://doi.org/10.3390/antiox10060837