대한본초학회지 (The Korea Journal of Herbology)

  • 제35권5호
  • /
  • Pages.33-39
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  • 2020
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  • 1229-1765(pISSN)
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  • 2288-7199(eISSN)
대한본초학회 (The Korea Association of Herbology)
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사이프러스 에센셜 오일의 흡입이 전임상 실험동물의 손상된 학습능력과 기억력에 미치는 영향

Cypress Essential Oil Improves Scopolamine-induced Learning and Memory Deficit in C57BL/6 mice

  • 이길용 (대구한의대학교 향산업전공) ;
  • 이찬 (계명대학교 의과대학 약리학교실) ;
  • 백정인 (대구한의대학교 향산업전공) ;
  • 배근영 (펜실베니아 대학교, 생명과학과 경영 복수 학위과정) ;
  • 박찬익 (대구한의대학교 향산업전공) ;
  • 장정희 (계명대학교 의과대학 약리학교실)
  • Lee, Gil-Yong (Department of Aroma-Applied Industry, Daegu Haany University) ;
  • Lee, Chan (Department of Pharmacology, School of Medicine, Keimyung University) ;
  • Baek, Jeong-In (Department of Aroma-Applied Industry, Daegu Haany University) ;
  • Bae, Keunyoung (University of Pennsylvania's Roy and Diana Vagelos Program in Life Sciences and Management) ;
  • Park, Chan-Ik (Department of Aroma-Applied Industry, Daegu Haany University) ;
  • Jang, Jung-Hee (Department of Pharmacology, School of Medicine, Keimyung University)
  • 투고 : 2020.03.26
  • 심사 : 2020.09.25
  • 발행 : 2020.09.30
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초록

Objectives : Increasing evidence supports the biological and pharmacological activities of essential oils on the central nervous system such as pain, anxiety, attention, arousal, relaxation, sedation and learning and memory. The purpose of present work is to investigate the protective effect and molecular mechanism of cypress essential oil (CEO) against scopolamine (SCO)-induced cognitive impairments in C57BL/6 mice. Methods : A series of behavior tests such as Morris water maze, passive avoidance, and fear conditioning tests were conducted to monitor learning and memory functions. Immunoblotting and RT-PCR were also performed in the hippocampal tissue to determine the underlying mechanism of CEO. Results : SCO induced cognitive impairments as assessed by decreased step-through latency in passive avoidance test, relatively low freezing time in fear conditioning test, and increased time spent to find the hidden platform in Morris water maze test. Conversely, CEO inhalation significantly reversed the SCO-induced cognitive impairments in C57BL/6 mice comparable to control levels. To elucidate the molecular mechanisms of memory enhancing effect of CEO we have examined the expression of brain-derived neurotrophic factor (BDNF) in the hippocampus. CEO effectively elevated the protein as well as mRNA expression of BDNF via activation of cAMP response element binding protein (CREB). Conclusions : Our findings suggest that CEO inhalation effectively restored the SCO-impaired cognitive functions in C56BL/6 mice. This learning and memory enhancing effect of CEO was partly mediated by up-regulation of BDNF via activation of CREB.

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참고문헌

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