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혼합영양 조건하에서 Haematococcus sp.의 배지 최적화 및 대사산물 생산

Medium Optimization for Cell Growth and Metabolite Formation from Haematococcus sp. under Mixotrophic Cultivation

  • Kim, Hyo Seon (Department of Biotechnology, Pukyong National University) ;
  • Kim, Sung-Koo (Department of Biotechnology, Pukyong National University) ;
  • Jeong, Gwi-Taek (Department of Biotechnology, Pukyong National University)
  • 투고 : 2020.04.22
  • 심사 : 2020.06.23
  • 발행 : 2020.09.28

초록

Haematococcus sp.를 대상으로 mixotroph 형태에서 배양을 위한 배지 최적화 연구를 수행하였다. 기본 배지로는 modified MS 배지가 적절했으며, 탄소원으로는 glucose가, 그리고 농도는 10 g/l가 적합하였다. 질소원으로는 KNO3를 선정하였으며, 농도는 1.9 g/l이 최적이었다. 최적의 배지조건에서 Haematococcus sp.를 초기 접종량(0.18 g/l)로 접종하여 14일 후에 5.58 ± 0.25 g/l로 성장하였으며, 이는 건조 세포중량 기준으로 약 31배의 성장한 것이다. 이때 생성된 클로로필은 172.16 ± 7.79 mg/l였으며, 카로티노이드는 42.33 ± 1.91 mg/l이었다. 본 연구의 결과는 추후 미세조류 대량 배양과 대사산물의 생산에 이용가능한 기초자료가 될 수 있을 것이라 판단된다.

In this study, the medium optimization for cell growth and metabolite formation of Haematococcus sp. under mixotrophic cultivation was investigated. As a result, modified MS medium was selected as the basal medium; glucose was selected as the carbon source, with an optimum concentration of 10 g/l, and potassium nitrate was chosen as the nitrogen source, with an optimum concentration of 1.9 g/l. Under optimum conditions, Haematococcus sp. demonstrated an increase in biomass concentration from 0.18 gDW/l to 5.58 gDW/l in 14 days, after which there was a 31-fold increase in its growth. At the same time, the concentrations of chlorophyll and carotenoids were 172.16 mg/l and 42.33 mg/l, respectively. This work will contribute to the basic data for mass cultivation of microalgae.

키워드

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