DOI QR코드

DOI QR Code

근육세포주에서 당 흡수 및 대사 조절에 대한 황기탕 에탄올 추출물의 효과

Effects of a Hwanggi-tang Ethanol Extract on Glucose Uptake and Metabolism in Murine Myotubes

  • 장철용 (원광대학교 한의과대학 내과학교실) ;
  • 신선호 (원광대학교 한의과대학 내과학교실) ;
  • 신용진 (원광대학교 한의과대학 내과학교실)
  • Jang, Chul-yong (Dept. of Internal Medicine, College of Korean Medicine, Wonkwang University) ;
  • Shin, Sun-ho (Dept. of Internal Medicine, College of Korean Medicine, Wonkwang University) ;
  • Shin, Yong-jeen (Dept. of Internal Medicine, College of Korean Medicine, Wonkwang University)
  • 투고 : 2020.08.19
  • 심사 : 2020.09.26
  • 발행 : 2020.09.30

초록

Objectives: The aim of this study was to evaluate the effects of Hwanggi-tang on glucose digestion, uptake, and metabolism in murine C2C12 myotubes. Methods: Hwanggi-tang was prepared according to the Dong-ui-bo-gam (≪東醫寶鑑≫) prescription by 70% ethanol extraction. The effect on glucose digestion was examined by determining the inhibitory effect of Hwanggi-tang on α-glucosidase activity. We also compared and verified the gene and protein expression of genes related to glucose uptake in C2C12 myotubes treated with Hwanggi-tang or insulin. Glucose metabolism was assessed by the expression levels of associated enzymes. Results: Hwanggi-tang caused a dose-dependent inhibition of α-glucosidase activity, induced glucose uptake by activation of the PI3K/Akt/mTOR pathway in the insulin signaling pathway, and promoted glucose oxidation and β-oxidation. Conclusions: Hwanggi-tang exerts an anti-diabetic effect on murine myotubes by inhibiting glucose digestion and inducing glucose uptake and consumption.

키워드

참고문헌

  1. American Diabetes Association. Diagnosis and Classification of Diabetes Mellitus. Diabetes Care 2014;37(Suppl. 1):S81-90. https://doi.org/10.2337/dc14-S081
  2. Department of internal medicine (Nephro-Endocrine system), College of Korean Medicine. Nephro-Endocrinology. Seoul: Koonja; 2011, p. 89.
  3. Kang SB. The Comparative Study between the Transformations(傳變症) of Sogal(消渴) and the Complications of Diabetes Mellitus. J of Korean Orient Med 1998;19(2):137-52.
  4. Heo J. Dongeuibogam. Hadong: Dongeuibogam publisher; 2005, p. 1451.
  5. 楊士瀛. 仁齋直指. 上海: 上海古籍出版社; 1991, p. 331.
  6. Ahn SW, Kim YG, Kim MH, Lee HY, Seong NS. Comparison of Biological Activities on Rehmannia Radix and R. Radix Preparata produced in Korea. Korean J medicinal crop sci 1999;7(4):257-62.
  7. Yin Y, Heo SI, Jung MJ, Wang MH. Antioxidant and Antidiabetic Effects of Various Sections of Astragalus membranaceus. Kor J Pharmacogn 2009;40(1):1-5.
  8. Song MY, Kim EK, Song JH. Protective Effect of Radix Trichosanthis Extracts on Cytotoxicity of Pancreatic ${\beta}$-Cells by Cytokines. Korean J Oriental Physiology & Pathology 2008;22(2):422-6.
  9. Kim OK. Antidiabetic Effect of Ethanol Extract of Liriope platyphylla in Streptozotocin Induced Diabetic Rats. J of Korean Oil Chemists Soc 2017;34(2):254-9.
  10. Ko BS, Park SK, Choi SB, Jun DW, Choi MK, Park SM. A Study on Hypoglycemic Effects of Crude Extracts of Schizandrae Fructus. J Korean Soc Appl Biol Chem 2004;47(2):258-64.
  11. Jang DE, Song J, Hwang IG, Lee SH, Choe JS, Hwang KA. Determination of Glycyrrhizic Acid Content and Anti-Diabetic Effect of Glycyrrhiza uralensis Depending on Cultivation Region. J Korean Soc Food Sci Nutr 2017;46(1):39-45. https://doi.org/10.3746/jkfn.2017.46.1.039
  12. Zhang L, Hogan S, Li J, Sun S, Canning C, Zheng SJ, Zhou K. Grape skin extract inhibits mammalian intestinal ${\alpha}$-glucosidase activity and suppresses postprandial glycemic response in streptozocin-treated mice. Food Chem 2011;126(2):466-71. https://doi.org/10.1016/j.foodchem.2010.11.016
  13. Jung DW, Ha HH, Zheng X, Chang YT, Williams DR. Novel use of fluorescent glucose analogues to identify a new class of triazine-based insulin mimetics possessing useful secondary effects. Mol Biosyst 2011;7(2):346-58. https://doi.org/10.1039/C0MB00089B
  14. Wang Q, Khayat Z, Kishi K, Ebina Y, Klip A. GLUT4 translocation by insulin in intact muscle cells: detection by a fast and quantitative assay. FEBS Lett 1998;427(2):193-7. https://doi.org/10.1016/S0014-5793(98)00423-2
  15. Calder PC, Geddes R. Acarbose is a competitive inhibitor of mammalian lysosomal acid alpha-Dglucosidases. Carbohydr Res 1989;191(1):71-8. https://doi.org/10.1016/0008-6215(89)85047-5
  16. Minister of Ministry of Health and Welfare. Korea Health Statistics 2018: Korea National Health and Nutrition Examination Survey (KNHANES VII-3). 2018. Sejong: Ministry of Health and Welfare; 2019, p. 241, 244, 250.
  17. Director of the Centers for Disease Control & Prevention. 2019 Chronic disease status and issues. 2019. Cheongju-si: Korean Centers for Disease Control & Prevention; 2019, p. 22, 33.
  18. The Korean Association of Internal Medicine. Drug prescription guide for Primary physician. Seoul: Korea Institute of Medicine; 2010, p. 455-60.
  19. Cho SY, Yoo WJ, Ahn SW, Kim NI. The formation of Sogal concept and classification in Korean Traditional Medicine. Korean journal of oriental medicine 2007;13(2):1-14.
  20. Choi YK, Systematic Reviews of Current Domestic Studies of Herbaceous Plants on Anti-diabetes - since 2000. Korean J Oriental Physiology & Pathology 2011;25(3):389-97.
  21. 王燾. 外臺秘要. 北京: 人民衛生出版社; 1996, p. 304.
  22. Shin MK. Clinical Traditional Herbalogy. Paju: Younglimsa; 2006, p. 173, 196, 251, 265, 280, 369, 650.
  23. Shobana S, Sreerama YN, Malleshi NG. Composition and enzyme inhibitory properties of finger millet (Eleusine coracana L.) seed coat phenolics: Mode of inhibition of alpha-glucosidase and pancreatic amylase. Food Chem 2009;115(4):1268-73. https://doi.org/10.1016/j.foodchem.2009.01.042
  24. Tokarz VL, MacDonald PE, Klip A. The cell biology of systemic insulin function. J Cell Biol 2018;217(7):2273-89. https://doi.org/10.1083/jcb.201802095
  25. Huang S, Czech MP. The GLUT4 glucose transporter. Cell Metab 2007;5(4):237-52. https://doi.org/10.1016/j.cmet.2007.03.006
  26. DeBerardinis RJ, Lum JJ, Hatzivassiliou G, Thompson CB. The biology of cancer: metabolic reprogramming fuels cell growth and proliferation. Cell Metab 2008;7(1):11-20. https://doi.org/10.1016/j.cmet.2007.10.002
  27. Quintela AM, Jimenez R, Piqueras L, Gomez-Guzman M, Haro J, Zarzuelo MJ, et al. $PPAR{\beta}$ activation restores the high glucose-induced impairment of insulin signalling in endothelial cells. Br J Pharmacol 2014;171(12):3089-102. https://doi.org/10.1111/bph.12646
  28. Wullschleger S, Loewith R, Hall MN. TOR signaling in growth and metabolism. Cell 2006;124(3):471-84. https://doi.org/10.1016/j.cell.2006.01.016
  29. Yip SC, Saha S, Chernoff J. PTP1B: a double agent in metabolism and oncogenesis. Trends Biochem Sci 2010;35(8):442-9. https://doi.org/10.1016/j.tibs.2010.03.004
  30. Goldstein BJ, Bittner-Kowalczyk A, White MF, Harbeck M. Tyrosine Dephosphorylation and Deactivation of Insulin Receptor Substrate-1 by Protein-tyrosine Phosphatase 1B. J Biol Chem 2000;275(6):4283-9. https://doi.org/10.1074/jbc.275.6.4283
  31. Seo YS, Shon MY, Kong R, Kang OH, Zhou T, Kim DY, Kwon DY. Black ginseng extract exerts anti-hyperglycemic effect via modulation of glucose metabolism in liver and muscle. J Ethnopharmacol 2016;190:231-40. https://doi.org/10.1016/j.jep.2016.05.060