KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Selection of Salt-Tolerant Silage Rice Through in vitro Screening and Saltol QTL Analysis

기내 선발과 Saltol QTL 분석을 통한 내염성 증진 사료용 벼 선발

  • Cho, Chuloh (Crop Foundation Research Division, National Institute of Crop Science, RDA) ;
  • Kim, Kyung Hwa (Crop Foundation Research Division, National Institute of Crop Science, RDA) ;
  • Ahn, Eok-Keun (Department of Central Area Crop Science, National Institute of Crop Science, RDA) ;
  • Park, Hyangmi (Department of Central Area Crop Science, National Institute of Crop Science, RDA) ;
  • Choi, Man-Soo (Crop Foundation Research Division, National Institute of Crop Science, RDA) ;
  • Chun, Jaebuhm (Crop Foundation Research Division, National Institute of Crop Science, RDA) ;
  • Seo, Mi-Suk (Crop Foundation Research Division, National Institute of Crop Science, RDA) ;
  • Jin, Mina (Crop Foundation Research Division, National Institute of Crop Science, RDA) ;
  • Kim, Dool-Yi (Crop Foundation Research Division, National Institute of Crop Science, RDA)
  • 조철오 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 김경화 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 안억근 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 박향미 (농촌진흥청 국립식량과학원 중부작물부) ;
  • 최만수 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 전재범 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 서미숙 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 진민아 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 김둘이 (농촌진흥청 국립식량과학원 작물기초기반과)
  • Received : 2020.05.18
  • Accepted : 2020.07.29
  • Published : 2020.09.01
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Abstract

Salinity is one of the major abiotic stressors that inhibits the growth, yield, and productivity of crop plants. Therefore, it is necessary to develop crops with increased salt tolerance for cultivation in saline soils such as is found in reclaimed land. The objective of this study was to develop a salt-tolerant silage rice line that grows on reclaimed land. In order to develop this salt-tolerant silage rice, we transferred Saltol, a major QTL associated with salt tolerance, from IR64-Saltol, a salt-tolerant indica variety, into Mogyang, a susceptible elite japonica variety. To determine the effect of salt stress, Mogyang and IR64-Saltol cultivars were grown on a medium containing various concentrations of NaCl in in vitro conditions. Shoot length was found to decrease with increasing salt concentrations, and root growth was almost arrested at NaCl concentrations over 50 mM in the Mogyang cultivar. Based on these preliminary results, we screened five salt-tolerant lines showing superior growth under salt stress conditions. Polymerase chain reaction and sequencing results showed that the introgression types of Saltol QTL were derived from the IR64-Saltol cultivar in almost all selected lines. Based on the observed growth and physiological characteristics, the new Saltol introgression lines showed higher salt tolerance compared to the Mogyang parental cultivar. The salt-tolerant lines identified in this study could be used as a genetic resource to improve rice salt tolerance.

본 연구에서는 간척지와 같은 염류집적 토양에서 재배가 가능한 사료용 벼 품종 개발을 위해 자포니카 우량품종 목양과 내염성 인디카 품종 IR64-Saltol 교배 계통으로부터 기내 선발 방법과 분자마커 분석을 통해 내염성 증진 계통을 선발하였고, 연구결과는 다음과 같다. 1. 목양과 IR64-Saltol 품종에 다양한 농도의 NaCl을 처리하여 신초 길이, 근장 및 생체중의 변화를 분석한 결과, 목양은 IR64-Saltol과 비교하여 50 mM NaCl 농도에서 신초 길이, 근장 및 생체중이 심한 생육 저해를 보였다. 2. 목양과 IR64-Saltol 교배 54계통 224개체를 이용하여 기내 선발 방법을 통해 50 mM NaCl 처리 후 목양 대비 신초 길이, 근장 및 생체중이 양호한 5계통(767883, 767885, 767949, 767986, 767989)을 선발하였다. 3. 내염성 관련 양적형질인 Saltol QTL의 유래를 확인하기 위한 분자마커 분석 결과와 표현형 결과를 비교하여 IR64-Saltol에서 유래된 Saltol QTL이 이입된 계통들은 목양 유래 Saltol QTL이 혼재된 계통들과 비교하여 염 스트레스 시 신초 길이, 근장 및 생체중이 양호함을 확인하였고, 따라서 IR64-Saltol 유래 Saltol QTL의 이입이 내염성을 증진시킨 것으로 판단된다. 4. 내염성 관련 핵심 유전자인 SKC1 발현은 염 처리 후 목양 대비 선발된 5계통 모두에서 높은 발현양을 보이나 목양 유래 QTL이 혼재된 2계통보다 IR64-Saltol QTL만 전이된 3계통에서 보다 높은 발현양을 보였다. 이러한 SKC1의 발현 양상이 선발된 계통들의 내염성에 관여할 것으로 판단된다. 5. 이상의 결과 기내 선발과 분자마커 분석을 통해 선발한 내염성 계통은 간척지와 같은 불량한 환경에서 작물 재배 및 생산이 가능한 내염성 품종 개발의 육종 소재로 활용될 수 있을 것으로 판단된다.

Keywords

References

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