Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
권호 표지
DOI QR코드

DOI QR Code

Effect of Lactobacillus acidophilus based probiotic product supplementation on the blood profile, fecal noxious gas emission, and fecal shedding of lactic acid bacteria and coliform bacteria in healthy adult Beagle dogs

  • Sun, Hao Yang (Department of Animal Resource and Science, Dankook University) ;
  • Kim, In Ho (Department of Animal Resource and Science, Dankook University)
  • Received : 2020.05.20
  • Accepted : 2020.07.03
  • Published : 2020.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

The aim of this study was to evaluate the effect of Lactobacillus acidophilus probiotic (LAP) product supplementation on the blood profile, fecal noxious gas emission, and fecal shedding of lactic acid bacteria and coliform bacteria in healthy adult Beagle dogs. In total, 14 Beagle dogs with an average initial body weight of 10.19 ± 0.61 kg were randomly assigned into two dietary treatments,with and without LAP supplementation, for a 28-day feeding trial. At the end of the experiment, there was no significant (p > 0.05) difference in the concentration of serum total cholesterol, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), white blood cell (WBC), red blood cell (RBC), blood lymphocyte percentage, fecal hydrogen sulfide (H2S) and total mercaptans (R.SH) emission, and fecal coliforms counts. However, the serum concentrations of the triglyceride and fecal ammonia (NH3) emission of the LAP treatment were significantly (p < 0.05) decreased in the group compared with the CON dogs. Fecal total lactic acid bacteria counts were significantly (p < 0.05) increased in the LAP treatment. In conclusion, the supplementation of LAP in Beagle dog diets could decrease the blood triglyceride level and enhance the gut Lactobacillus count which may have positive effects on dogs.

Keywords

References

  1. AAFCO (Association of American Feed Control Officials). 2009. Dog and cat food feeding protocols. Official Publication, Oxford, IN, USA.
  2. Abd El-Gawad IA, El-Sayed EM, Hafez SA, El-Zeini HM, Saleh FA. 2005. The hypocholesterolaemic effect of milk yoghurt and soy-yoghurt containing bifidobacteria in rats fed on a cholesterol-enriched diet. International Dairy Journal 15:37-44. https://doi.org/10.1016/j.idairyj.2004.06.001
  3. Ahansan ASML, Agazzi A, Invernizzi G, Bontempo V, Savoini G. 2015. The beneficial role of probiotics in monogastric animal nutrition and health. Journal of Dairy Veterinary and Animal Research 24:116-132.
  4. Altermann E, Russell WM, Azcarate-Peril MA, Barrangou R, Buck BL, Mc Auliffe O, Lick S. 2005. Complete genome sequence of the probiotic lactic acid bacterium Lactobacillus acidophilus NCFM. Proceedings of the National Academy of Sciences 102:3906-3912. https://doi.org/10.1073/pnas.0409188102
  5. Andoh A, Fujiyama Y. 2006. Therapeutic approaches targeting intestinal microflora in inflammatory bowel disease. World Journal of Gastroenterology 12:4452-4460. https://doi.org/10.3748/wjg.v12.i28.4452
  6. Baillon MLA, Marshall-Jones ZV, Butterwick RF. 2004. Effects of probiotic Lactobacillus acidophilus strain DSM13241 in healthy adult dogs. American Journal of Veterinary Research 65:338-343. https://doi.org/10.2460/ajvr.2004.65.338
  7. Bontempo V. 2005. Nutrition and health of dogs and cats: Evolution of pet food. Veterinary Research Communications 29:45-50. https://doi.org/10.1007/s11259-005-0010-8
  8. Ferket PR, Van Heugten E, Van Kempen TATG, Angel R. 2002. Nutritional strategies to reduce environmental emissions from nonruminants. Journal of Animal Science 80:E168-E182. https://doi.org/10.2527/animalsci2002.80E-Suppl_2E168x
  9. Fuller R. 2001. The chicken gut microflora and probiotic supplements. The Journal of Poultry Science 38:189-196. https://doi.org/10.2141/jpsa.38.189
  10. Fuller R, Barrow PA, Brooker BE. 1978. Bacteria associated with the gastric epithelium of neonatal pigs. Applied and Environmental Microbiology 35:582-591. https://doi.org/10.1128/AEM.35.3.582-591.1978
  11. Gonzalez SN, Apella MC, Romero N, Pesce de Ruiz Holgado AA, Oliver G. 1989. Superoxide dismutase activity in some strains of lactobacilli: Induction by manganese. Chemical and Pharmaceutical Bulletin 37:3026-3028. https://doi.org/10.1248/cpb.37.3026
  12. Hossain MM, Begum M, Kim IH. 2015. Effect of Bacillus subtilis, Clostridium butyricum and Lactobacillus acidophilus endospores on growth performance, nutrient digestibility, meat quality, relative organ weight, microbial shedding and excreta noxious gas emission in broilers. Veterinarni Medicina 60:77-86. https://doi.org/10.17221/7981-VETMED
  13. Kalavathy R, Abdullah N, Jalaludin S, Ho YW. 2003. Effects of Lactobacillus cultures on growth performance, abdominal fat deposition, serum lipids and weight of organs of broiler chickens. British Poultry Science 44:139-144. https://doi.org/10.1080/0007166031000085445
  14. Lan PTN, Sakamoto M, Benno Y. 2004. Effects of two probiotic Lactobacillus strains on jejunal and cecal microbiota of broiler chicken under acute heat stress condition as revealed by molecular analysis of 16S rRNA genes. Microbiology and Immunology 48:917-929. https://doi.org/10.1111/j.1348-0421.2004.tb03620.x
  15. Lan R, Koo J, Kim I. 2017a. Effects of Lactobacillus acidophilus supplementation on growth performance, nutrient digestibility, fecal microbial and noxious gas emission in weaning pigs. Journal of the Science of Food and Agriculture 97:1310-1315. https://doi.org/10.1002/jsfa.7866
  16. Lan RX, Lee SI, Kim IH. 2016. Effects of multistrain probiotics on growth performance, nutrient digestibility, blood profiles, faecal microbial shedding, faecal score and noxious gas emission in weaning pigs. Journal of Animal Physiology and Animal Nutrition 100:1130-1138. https://doi.org/10.1111/jpn.12501
  17. Lan RX, Lee SI, Kim IH. 2017b. Effects of Enterococcus faecium SLB 120 on growth performance, blood parameters, relative organ weight, breast muscle meat quality, excreta microbiota shedding, and noxious gas emission in broilers. Poultry Science 96:3246-3253. https://doi.org/10.3382/ps/pex101
  18. Liu H, Ji HF, Zhang DY, Wang SX, Wang J, Shan DC, Wang YM. 2015. Effects of Lactobacillus brevis preparation on growth performance, fecal microflora and serum profile in weaned pigs. Livestock Science 178:251-254. https://doi.org/10.1016/j.livsci.2015.06.002
  19. Liu W, Devi S, Park J, Kim I. 2018a. Effects of complex probiotic supplementation in growing pig diets with and without palm kernel expellers on growth performance, nutrient digestibility, blood parameters, fecal microbial shedding and noxious gas emission. Animal Science Journal 89:552-560. https://doi.org/10.1111/asj.12965
  20. Liu WC, Kim IH. 2018. Effects of different dietary n-6: n-3 PUFA ratios on growth performance, blood lipid profiles, fatty acid composition of pork, carcass traits and meat quality in finishing pigs. Annals of Animal Science 18:143-154. https://doi.org/10.1515/aoas-2017-0026
  21. Liu WC, Ye M, Liao JH, Zhao ZH, Kim IH, An LL. 2018b. Application of complex probiotics in swine nutrition-A review. Annals of Animal Science 18:335-350. https://doi.org/10.2478/aoas-2018-0005
  22. Ljungh A, Wadstrom T. 2006. Lactic acid bacteria as probiotics. Current Issues in Intestinal Microbiology 72:73-90.
  23. Maldonado EN, Casanave EB, Aveldano MI. 2002. Major plasma lipids and fatty acids in four HDL mammals. Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology 132:297-303. https://doi.org/10.1016/S1095-6433(02)00031-4
  24. McCoy S, Gilliland SE. 2007. Isolation and characterization of Lactobacillus species having potential for use as probiotic cultures for dogs. Journal of Food Science 72:M94-M97. https://doi.org/10.1111/j.1750-3841.2007.00310.x
  25. Munyaka PM, Nandha NK, Kiarie E, Nyachoti CM, Khafipour E. 2015. Impact of combined ${\beta}$-glucanase and xylanase enzymes on growth performance, nutrients utilization and gut microbiota in broiler chickens fed corn or wheatbased diets. Poultry Science 95:528-540. https://doi.org/10.3382/ps/pev333
  26. NRC (National Research Council). 2006. Nutrient requirements of dogs and cats. National Academies Press, Washington, D.C., USA.
  27. Qiao J, Li H, Wang Z, Wang W. 2015. Effects of Lactobacillus acidophilus dietary supplementation on the performance, intestinal barrier function, rectal microflora and serum immune function in weaned piglets challenged with Escherichia coli lipopolysaccharide. Antonie van Leeuwenhoek 107:883-891. https://doi.org/10.1007/s10482-015-0380-z
  28. Strompfova V, Kubasova I, Laukova A. 2017. Health benefits observed after probiotic Lactobacillus fermentum CCM 7421 application in dogs. Applied Microbiology and Biotechnology 101:6309-6319. https://doi.org/10.1007/s00253-017-8425-z
  29. Tsujii H, Kuwabara Y, Salma U, Miah AG, Nishimura M, Maki T. 2008. Effect of dietary Rhodobacter capsulatus on cholesterol, triglycerides concentration, and meat quality of finishing pigs. Animal Science Journal 79:460-465. https://doi.org/10.1111/j.1740-0929.2008.00550.x
  30. Uyeno Y, Shigemori S, Shimosato T. 2015. Effect of probiotics/prebiotics on cattle health and productivity. Microbes and Environments 30:126-132. https://doi.org/10.1264/jsme2.ME14176
  31. Zhang JL, Xie QM, Ji J, Yang WH, Wu YB, Li C, Bi YZ. 2012. Different combinations of probiotics improve the production performance, egg quality, and immune response of layer hens. Poultry Science 91:2755-2760. https://doi.org/10.3382/ps.2012-02339