DOI QR코드

DOI QR Code

Anti-oxidation and Fat Accumulation Inhibitory Effects of Silbi-san

실비산의 항산화 및 지방축적 억제 효과

  • Kim, Geon-Woo (Department of Korean Rehabilitation Medicine, Graduate School Daegu Haany University) ;
  • Woo, Chang-Hoon (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine) ;
  • Kim, Young-Jun (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine) ;
  • An, Hee-Duk (Department of Rehabilitation Medicine of Korean Medicine, College of Korean Medicine)
  • 김건우 (대구한의대학교 대학원 한의학과 한방재활의학과학) ;
  • 우창훈 (대구한의대학교 한의과대학 한방재활의학과교실) ;
  • 김영준 (대구한의대학교 한의과대학 한방재활의학과교실) ;
  • 안희덕 (대구한의대학교 한의과대학 한방재활의학과교실)
  • Received : 2020.09.23
  • Accepted : 2020.10.22
  • Published : 2020.10.31

Abstract

Objectives The purpose of this study was to investigate the effects of Silbi-san on the antioxidant and fat accumulation inhibition and to analyze the anti-obesity effect by analyzing the changes in serum lipid composition in obese mice. Methods We compared contents of phytochemicals like total polyphenols and total flavonoid and antioxidant activities such as 2,2-dipheny-1-picrylhydrazyl and 2.2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activity. After Silbi-san in 3T3-L1 cells in vitro and mouse adipose tissue ex vivo, we quantified intracellular triglyceride accumulation and lipolysis. Moreover, the anti-obesity activity though inhibiting pancreatic lipase were analyzed. In 3T3-L1 cells, morphological changes showed that control cells had many lipid while cells treated with Silbi-san had less lipid accumulation. 30% EtOH Silbi-san treatment also suppressed the fat absorption by inhibiting the activity of pancreatic lipase and led to high lipolysis through promoting glycerol release. The experimental group was divided into four groups: Normal group fed normal feed, Control group fed 60% high fat diet (HFD) and distilled water, drug group fed 60% high fat diet and 200 mg/kg of Silbi-san water extract, drug group fed 60% HFD and 200 mg/kg of Silbi-san 30% ethanol extract. Results Serum total cholesterol content and serum low density lipoprotein-cholesterol content were significantly decreased in the Silbi-san extract group compared to the control group, serum high density lipoprotein-cholesterol content was significantly increased in Silbi-san extract group. Conclusions In this study, the antioxidant and fat accumulation inhibitory effects of Silbi-san were confirmed.

Keywords

References

  1. Korea Institute of Oriental Medicine. Clinical practice guideline for obesity in Korean Medicine. Seoul:Elsevier. 2016:30.
  2. World Health Organization (WHO). Obesity and overweight. Geneva:WHO. 2018 [cited 2019 Oct 31]. Available from: https://www.who.int/en/news-room/fact-sheets/detail/obesity-and-overweight.
  3. Korea National Statistical Office. Prevalence of obesity. Seoul:Korea national statistical office. 2017 [cited 2019 Oct 31]. Available from: http://m.kosis.kr/mobService/data/NewSeriesJipyoListInfo01.do?parmMainJipyo=514&preListNm=%EB%B3%B4%EA%B1%B4%C2%B7%EB%B3%B5%EC%A7%80&checkFlag=N.
  4. Wang B. Hwangjenaegyeongsomun. Seoul:Daesungmunhwasa. 1994:93.
  5. Kim S, Jin SH, Kim TH. A study on etiological factors, symptoms of a disease, and treatments of obesity based on Dong-Yi Bo Gam. J Korean Med. 1998;19(2):125-36.
  6. Heo J. Donguibogam. Seoul:beobinmunhwasa. 1999:1306.
  7. Hatano T, Edamatsu R, Hiramatsu M, Mori A, Fujita Y, Yasuhara T, Yoshida T, Okuda T. Effects of the interaction of tannins with co-existing substances. VI. Effects of tannins and related polyphenols on superoxide anion radical, and on 1,1-diphenyl-2-picrylhydrazyl radical. Chem Pharm Bull. 1989;37(8):2016-21. https://doi.org/10.1248/cpb.37.2016
  8. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999;26(9-10):1231-7. https://doi.org/10.1016/S0891-5849(98)00315-3
  9. Folin O, Denis W. On phosphotungstic-phosphomolybdic compounds as color reagents. J Biol Chem. 1912;12:239-43. https://doi.org/10.1016/S0021-9258(18)88697-5
  10. Lister CE, Lancaster JE, Sutton KH, Walker JR. Developmental changes in the concentration and composition of flavonoids in skin of a red and a green apple cultivar. J Sci Food Agric. 1994;64(2):155-61. https://doi.org/10.1002/jsfa.2740640204
  11. Kim JH, Kim HJ, Park HW, Youn SH, Choi DY, Shin CS. Development of inhibitors against lipase and alpha-glucosidase from derivatives of monascus pigment. FEMS Microbiol Lett. 2007;276(1):93-8. https://doi.org/10.1111/j.1574-6968.2007.00917.x
  12. Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem. 1972;18(6):499-502. https://doi.org/10.1093/clinchem/18.6.499
  13. Mihara M, Uchiyama M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem. 1978;86(1):271-8. https://doi.org/10.1016/0003-2697(78)90342-1
  14. Korea Disease Control and Prevention Agency. Korea national health & nutrition examination survey, Youth health behavior survey in 2018. 2018 [cited 2019 Oct 31]. Available from: https://knhanes.cdc.go.kr/knhanes/sub04/sub04_04.do.
  15. Korean Society for the Study of Obesity. Clinical obesity. Seoul:Korea Medical Book Publishing Company. 2008:143, 180.
  16. Tak YJ, Lee YS, Lee JS, Kang JH. The treand of obesity-related researches in Korea:from 1984 to 2002. Korean J Obes. 2004;13(1):1-13.
  17. Hwang MJ, Shin HD, Song MY. Literature review of herbal medicines on treatment of obesity since 2000; mainly about Ephedra Herba. J Korean Med Obes Res. 2007;7(1):39-54.
  18. Shin JY. Bangyakhappyeon commentary. Gyeonggido:Sungbosa. 2005:134.
  19. Kim HJ, Lee YM, Kim YH, Won SI, Choi SA, Choi SW. Inhibition of adipogenesis in 3T3-L1 adipocytes with Magnolia officinalis extracts. J Soc Cosmet Scientists Korea. 2009;35(2):117-23.
  20. Kim DH, Kwon BR, K im S J, K im HJ, J eong S I, Yu KY, Kim SY. Anti-obese effects and signaling mechanisms of Chaenomeles sinensis extracts in 3T3-L1 preadipocytes and obese mice fed a high-fat diet. Herb Formula Sci. 2017;25(4):457-69. https://doi.org/10.14374/HFS.2017.25.4.457
  21. Lee JA, Park YJ, Jeong WS, Hong SS, Ahn EK. Anti-obesity effect of Amomum taso-ko ethanol extract in 3T3-L1 adipocytes. J Appl Biol Chem. 2017;60(1):23-8. https://doi.org/10.3839/jabc.2017.005
  22. Ryu EK, Chung SH, Lee JS, Kim SS, Shin HD. Effects of Poria cocos extract on the changes of the weight, epididymal fat tissue, serum and m-RNA in visceral fat of the obese rats induced by fat diet. J Oriental Rehab Med. 2000;10(2):81-98.
  23. Yoon TS, Sung YY, Jang JY, Yang WK, Ji YU, Kim HK. Anti-obesity activity of extract from Saussurea lappa. Korean J Medicinal Crop Sci. 2010;18(3):151-6.
  24. Que F, Mao L, Zhu C, Xie G. Antioxidant properties of chinese yellow wine, its concentrate and volatiles. LWT-Food Science and Technology. 2006;39(2):111-7. https://doi.org/10.1016/j.lwt.2005.01.001
  25. Kim MJ, Park EJ. Feature analysis of different in vitro antioxidant capacity assays and their application to fruit and vegetable samples. J Korean Soc Food Sci Nutr. 2011;40(7):1053-62. https://doi.org/10.3746/jkfn.2011.40.7.1053
  26. Awika JM, Rooney LW, W u X, P rior R L, C isneros-Zevallos L. Screening methods to measure antioxidant activity of sorghum (sorghum bicolor) and sorghum products. J Agric Food Chem. 2003;51(23):6657-62. https://doi.org/10.1021/jf034790i
  27. Pohanka M, Pejchal J, Snopkova S, Havlickova K, Karasova JZ, Bostik P, Pikula J. Ascorbic acid: an old player with a broad impact on body physiology including oxidative stress suppression and immunomodulation: a review. Mini Rev Med Chem. 2012;12(1):35-43. https://doi.org/10.2174/138955712798868986
  28. Jeon WY, L ee MY, L im HS, S hin IS, Kim YJ, Jin SE, You SR, Seo CS, Kim JH, Ha HK, Jeong SJ, Kim OS, Shin NR, Kim SS, Shin HK. Experimental studies on the inflammation-related diseases pharmacological effect of water and 70% ethanol extracts from Socheongnyong-tang. Herb Formula Sci. 2012;20(2):13-28. https://doi.org/10.14374/HFS.2012.20.2.013
  29. Weon JB, Lee JW, Eom MR, Jung YS, Ko HJ, Lee HY, Park DS, Chung HC, Chung JY, Ma CJ. Effect of water and ethanol extracts Codonopsis lanceolata on spatial learning and memory in mice. Yakhak Hoeji. 2014;58(5):287-93.
  30. Dai J, Mumper RJ. Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules. 2010;15(10):7313-52. https://doi.org/10.3390/molecules15107313
  31. Kim EJ, Choi JY, Yu MR, Kim MY, Lee SH, Lee BH. Total polyphenols, total flavonoid contents, and antioxidant activity of korean natural and medicinal plants. Korean J Food Sci Technol. 2012;44(3):337-42. https://doi.org/10.9721/KJFST.2012.44.3.337
  32. Lee SJ, Chung HY, Lee IK, Yoo ID. Isolation and identification of flavonoids from ethanol extracts of artemisia vulgaris and their antioxidant activity. Korean J Food Sci Technol. 1999;31(3):815-22.
  33. Floegel A, Kim DO, Chung SJ, Koo SI, Chun OK. Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. Journal of Food Composition and Analysis. 2011;24(7):1043-8. https://doi.org/10.1016/j.jfca.2011.01.008
  34. Lee SH. Evaluation of asymptomatic hyperamylasemia and hyperlipasemia. Korean J Pancreas Biliary Tract. 2017;22(3):103-13. https://doi.org/10.15279/kpba.2017.22.3.103
  35. Woo SE, Kwon JH, Yang SY, Park HJ, Kim HK. Development of egg yolk antibody specific to the pancreatic lipase domain for anti-obesity. Korean J Microbiol Biotechnol. 2008;36(4):299-306.
  36. Lsselbacher KJ,Braunwald E, Wilson JD, Martin JB, Fauci AS, Kasper DL. Harrison's principles of internal medicine. Seoul:MIP. 2006:1554-63, 2500-12.