Journal of Ginseng Research

  • 제44권5호
  • /
  • Pages.738-746
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  • 2020
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  • 1226-8453(pISSN)
  • /
  • 2093-4947(eISSN)
고려인삼학회 (The Korean Society of Ginseng)
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Effects of red ginseng on the elastic properties of human skin

  • Park, Moon Young (Department of Biomedical Engineering, College of Medicine, Kyung Hee University) ;
  • Han, Se Jik (Department of Biomedical Engineering, College of Medicine, Kyung Hee University) ;
  • Moon, Donggerami (Department of Biomedical Engineering, College of Medicine, Kyung Hee University) ;
  • Kwon, Sangwoo (Department of Biomedical Engineering, College of Medicine, Kyung Hee University) ;
  • Lee, Jin-Woo (Medical Science Research Institute, Kyung Hee University Medical Center) ;
  • Kim, Kyung Sook (Department of Biomedical Engineering, College of Medicine, Kyung Hee University)
  • 투고 : 2019.02.19
  • 심사 : 2019.08.14
  • 발행 : 2020.09.15
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초록

Background: Red ginseng contains components, including microelements, vitamins, essential oils, and fatty acids, that can be used in skincare to delay the aging process. We investigated the effects of red ginseng treatment on skin elasticity by assessing cellular stiffness and measuring collagen protein synthesis. Methods: Human dermal fibroblasts were treated with red ginseng, and the resulting changes in stiffness were investigated using atomic force microscopy. Cytoskeletal changes and mRNA expression of biomarkers of aging, including that of procollagens I and VII, elastin, and fibrillin-1, were investigated. Collagen in a human skin equivalent treated with red ginseng was visualized via hematoxylin and eosin staining, scanning electron microscopy, and atomic force microscopy. Results and conclusion: The stiffness of fibroblasts was significantly reduced by treatment with red ginseng concentrations of ≥ 0.8 mg/mL. The ratio of F-actin to G-actin decreased after treatment, which corresponded to a change in fibroblast stiffness. The storage modulus (G') and loss modulus (G'') of the skin equivalent were both lowered by red ginseng treatment. This result indicates that the viscoelasticity of the skin equivalent can be restored by red ginseng treatment.

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참고문헌

  1. Ganceviciene R, Liakou AI, Theodoridis A, Makrantonaki E, Zouboulis CC. Skin anti-aging strategies. Dermatoendocrinol 2012;4:308-19. https://doi.org/10.4161/derm.22804
  2. Kim J, Cho SY, Kim SH, Cho D, Kim S, Park CW, Shimizu T, Cho JY, Seo DB, Shin SS. Effects of Korean ginseng berry on skin antipigmentation and antiaging via FoxO3a activation. J Ginseng Res 2017;41:277-83. https://doi.org/10.1016/j.jgr.2016.05.005
  3. Gunin AG, Petrov VV, Vasilieva OV, Golubtsova NN. Age-related changes of blood vessels in the human dermis. Adv Gerontol 2015;5:65-71. https://doi.org/10.1134/S2079057015020058
  4. Sator PG. Skin treatments and dermatological procedures to promote youthful skin. Clin Interv Aging 2006;1:51-6. https://doi.org/10.2147/ciia.2006.1.1.51
  5. Diridollou S, Vabre V, Berson M, Vaillant L, Black D, Lagarde JM, Gregoire JM, Gall Y, Patat F. Skin ageing: changes of physical properties of human skin in vivo. Int J Cosmet Sci 2001;23:353-62. https://doi.org/10.1046/j.0412-5463.2001.00105.x
  6. Cao J, Pham DK, Tonge L, Nicolau DV. Study of atomic force microscopy forcee distance curves by simulation using the Connolly surface for proteins. Smart Mater Struct 2002;11:1-5. https://doi.org/10.1088/0964-1726/11/1/301
  7. Cappella B, Dietler G. Force-distance curves by atomic force microscopy. Surface Science Reports 1999;34:1-104. https://doi.org/10.1016/S0167-5729(99)00003-5
  8. Jerome G, Francis OS. The structure of human skin collagen as studied with the electron microscopy. J Exp Med 1948;88(5):555-68. https://doi.org/10.1084/jem.88.5.555
  9. Longo VD, Kennedy BK. Sirtuins in aging and age-related disease. Cell 2006;126:257-68. https://doi.org/10.1016/j.cell.2006.07.002
  10. Watson RE, Griffiths CE, Craven NM, Shuttleworth CA, Kielty CM. Fibrillin-rich microfibrils are reduced in photoaged skin. Distribution at the dermalepidermal junction. J Invest Dermatol 1999;112(5):782-7. https://doi.org/10.1046/j.1523-1747.1999.00562.x
  11. Park SY, Shin YK, Kim HT, Kim YM, Lee DG, Hwang ES, Cho BG, Yin CS, Kim KY, Yi TH. A single-center, randomized, double-blind, placebo-controlled study on the efficacy and safety of "enzyme-treated red ginseng powder complex (BG11001)" for antiwrinkle and proelasticity in individuals with healthy skin. J Ginseng Res 2016;40:260-8. https://doi.org/10.1016/j.jgr.2015.08.006
  12. Jeong HC, Hong HD, Kim YC, Rhee YK, Choi SY, Kim KT, Kim SS, Lee YC, Cho CW. Quantification of maltol in Korean ginseng (Panax ginseng) products by high-performance liquid chromatography-diode array detector. Pharmacogn Mag 2015;11:657-64. https://doi.org/10.4103/0973-1296.160452
  13. Jeon JM, Choi SK, Kim YJ, Jang SJ, Cheon JW, Lee HS. Antioxidant and antiaging effect of ginseng berry extract fermented by lactic acid bacteria. J Soc Cosmet Sci Korea 2011;37:75-81. https://doi.org/10.15230/SCSK.2011.37.1.075
  14. Lee CH, Kim HI, Kim JS, Oh MJ, Kim SW, Ma SY, Kim MS, Kwon J, Jeong HS, Oh CH. Morphological studies on the inhibitory effects of photoaging skin of fermented red ginseng in hairless mice. Korean J Orient Physiol Pathol 2014;28:206-16.
  15. Cappella B, Dietler G. Force-distance curves by atomic force microscopy. Surf Sci Rep 1999;34:1-104. https://doi.org/10.1016/S0167-5729(99)00003-5
  16. Cole CN, Scarcelli JJ. Transport of messenger RNA from the nucleus to the cytoplasm. Curr Opin Cell Biol 2006;18(3):299-306. https://doi.org/10.1016/j.ceb.2006.04.006
  17. Yamauchi M, Woodley DT, Mechanic GL. Aging and cross-linking of skin collagen. Biochem Biophys Res Commun 1988;152:898-903. https://doi.org/10.1016/S0006-291X(88)80124-4
  18. Quan T, Fisher GJ. Role of age-associated alterations of the dermal extracellular matrix microenvironment in human skin aging: a mini-review. Gerontology 2015;61:427-34. https://doi.org/10.1159/000371708
  19. Zaleska-Dorobisz U, Kaczorowski K, Pawlus A, Puchalska A, Inglot M. Ultrasound elastography: review of techniques and its clinical applications. Adv Clin Exp Med 2014;23:645-55. https://doi.org/10.17219/acem/26301
  20. Sarvazyan AP, Rudenko OV, Swanson SD, Fowlkes JB, Emelianov SY. Shear wave elasticity imaging: a new ultrasonic technology of medical diagnostics. Ultrasound Med Biol 1998;24:1419-35. https://doi.org/10.1016/S0301-5629(98)00110-0
  21. Ophir J, Cespedes I, Ponnekanti H, Yazdi Y, Li X. Elastography: a quantitative method for imaging the elasticity of biological tissues. Ultrason Imag 1991;13:111-34. https://doi.org/10.1177/016173469101300201
  22. Woo MS, Moon KJ, Jung HY, Park SR, Moon TK, Kim NS, Lee BC. Comparison of skin elasticity test results from the ballistometer and cutometer. Skin Res Technol 2014;20:422-8. https://doi.org/10.1111/srt.12134
  23. Bonaparte JP, Ellis D, Chung J. The effect of probe to skin contact force on cutometer MPA 580 measurements. J Med Eng Technol 2013;37:208-12. https://doi.org/10.3109/03091902.2013.779325
  24. Li C, Guan G, Reif R, Huang Z, Wang RK. Determining elastic properties of skin by measuring surface waves from an impulse mechanical stimulus using phase-sensitive optical coherence tomography. J R Soc Interface 2012;9:831-41. https://doi.org/10.1098/rsif.2011.0583
  25. Schulze C, Wetzel F, Kueper T, Malsen A, Muhr G, Jaspers S, Blatt T, Wittern KP, Wenck H, Kas JA. Stiffening of human skin fibroblasts with age. Biophys J 2010;99:2434-42. https://doi.org/10.1016/j.bpj.2010.08.026
  26. Ida DM, Monika P, Katarzyna P, Malgorzata L, Irena E, Malgorzata L. Agerelated changes in the mechanical properties of human fibroblasts and its prospective reversal after anti-wrinkle tripeptide treatment. Int J Pept Res Ther 2014;20:77-85. https://doi.org/10.1007/s10989-013-9370-z
  27. Lomri A, Marie PJ, Escurat M, Portier MM. Cytoskeletal protein synthesis and organization in cultured mouse osteoblastic cells. Effects of cell density. FEBS Lett 1987;222:311-6. https://doi.org/10.1016/0014-5793(87)80392-7
  28. Fallqvist B, Fielden ML, Pettersson T, Nordgren N, Kroon M, Gad AKB. Experimental and computational assessment of F-actin influence in regulating cellular stiffness and relaxation behaviour of fibroblasts. J Mech Behav Biomed Mater 2016;59:168-84. https://doi.org/10.1016/j.jmbbm.2015.11.039
  29. Schaefer A, Te Riet J, Ritz K, Hoogenboezem M, Anthony EC, Mul FP, de Vries CJ, Daemen MJ, Figdor CG, van Buul JD, et al. Actin-binding proteins differentially regulate endothelial cell stiffness, ICAM-1 function and neutrophil transmigration. J Cell Sci 2014;127:4470-82. https://doi.org/10.1242/jcs.154708
  30. Dominguez R, Holmes KC. Actin structure and function. Annu Rev Biophys 2011;40:169-86. https://doi.org/10.1146/annurev-biophys-042910-155359
  31. Huy TX, Reyes AW, Hop HT, Arayan LT, Min W, Lee HJ, Rhee MH, Chang HH, Kim S. Intracellular trafficking modulation by ginsenoside Rg3 inhibits Brucella abortus uptake and intracellular survival within RAW 264.7 cells. J Microbiol Biotechnol 2017 28;27:616-23. https://doi.org/10.4014/jmb.1609.09060
  32. Moey M, Rajapurohitam V, Zeidan A, Karmazyn M. Ginseng (Panax quinquefolius) attenuates leptin-induced cardiac hypertrophy through inhibition of p115Rho guanine nucleotide exchange factor-RhoA/Rho-associated, coiledcoil containing protein kinase-dependent mitogen-activated protein kinase pathway activation. J Pharmacol Exp Ther 2011;339:746-56. https://doi.org/10.1124/jpet.111.182600
  33. Gelse K, Poschl E, Aigner T. Collagensestructure, function, and biosynthesis. Adv Drug Deliv Rev 2003;55(12):1531-46. https://doi.org/10.1016/j.addr.2003.08.002
  34. Angel P, Szabowski A, Schorpp-Kistner M. Function and regulation of AP-1 subunits in skin physiology and pathology. Oncogene 2001;20:2413-23. https://doi.org/10.1038/sj.onc.1204380
  35. Pinnell SR. Regulation of collagen synthesis. J Invest Dermatol 1982;79(Suppl 1):73s-6s. https://doi.org/10.1111/1523-1747.ep12545835
  36. Yoko A, Yoshiro Y, Yusuke D, Yoshinobu I, Shigehiro N. Analysis of viscoelasticity of human skin for prevention of pressure ulcers. J Mech Med Biol 2008;8:33-43. https://doi.org/10.1142/S0219519408002383

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