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The Evaluation on the Effectiveness of Zingiber mioga extract as a Cosmetic Material through Verification of Cosmeceutical Activations

양하(Zingiber mioga) 추출물의 화장품 약리활성 검증을 통한 화장품 소재로서의 유효성 평가

  • Um, Mi Sun (Department of Beauty Care, Jangan University)
  • 엄미선 (장안대학교 뷰티케어과)
  • Received : 2020.08.27
  • Accepted : 2020.10.19
  • Published : 2020.10.31

Abstract

The purpose of this study was to confirm the possiblility of Zingiber mioga as a cosmetic material. For this we carried out biologically activated material characteristic evaluation about anti-oxidant, anti-inflammatory, wrinkle reduction effects using Zingiber mioga extract. To carry out this experiment, we extracted Zingiber mioga extract from Zingiber mioga flowers exract (ZMF) and Zingiber mioga leaves exract (ZML) with 70% ethanol. In order to evaluate the anti-inflammatory effect, we tested the toxicity and the hindrance activity to nitric oxide of samples using macrophages (RAW 264.7 cells). After we measured DPPH radical scavenging activity, ABTS+ radical scavenging activity and superoxide dismutase (SOD)-like activity of the Zingiber mioga extracts, we knew that they increased depending on their concentration. ZMF showed higher antioxidant activity than ZML after the measurement of ABTS+ radial scavenging activity and superoxide desmutase (SOD)-like activity. According to the measurement result of Nitric oxide inhibition activity we knew that ZMF reduced NO productions in a concentration-dependent manner. After the measurement of the biosynthesis quantity of pro-collagen type-1, we knew that its excellent effect appeared 110% or more at the concentration of 25 ㎍/ml. And at the same concentration, the result of the measurement of metalloprotease (MMP)-1 inhibition effect showed the 20% activation. In conclusion, ZMF is expected to be applied as a cosmetic material for wrinkle reduction. Zingiber mioga is believed to be used as a natural cosmetic material because it has been proven to have antioxidant, anti-inflammatory, and wrinkle-improving effects.

본 연구는 화장품 소재로서 양하의 가능성을 확인하기 위한 것이다. 이를 위해 우리는 양하추출물을 사용하여 항산화, 항염증, 주름개선 효과에 대한 생리 활성 평가를 실시하였다. 이 실험을 하기 위해, 양하꽃 추출물 (ZMF)과 양하잎 추출물 (ZML)을 70% 에탄올로 추출하였다. 항염증 효과를 알아보기 위해 macrophage (Raw 264.7)를 이용해 시료의 세포독성 평가와 nitric oxide 저해능을 측정하였다. ZMF과 ZML의 DPPH 라디칼 소거능, ABTS+ 라디칼 소거능, SOD) 유사 활성 측정 결과 농도 의존적으로 활성이 증가하였다. ZMF는 ABTS+ radical scavenging activity와 superoxide dismutase (SOD)-like activity 측정 결과 ZML보다 높은 항산화 활성을 보였다. NO 저해능 측정 결과에 따르면 ZMF는 농도 의존적으로 NO가 저해되어 우수한 항염증 효과를 나타냈다. ZMF의 pro-collagen type-1 합성량은 25 ㎍/ml에서 110% 이상의 우수한 효과를 나타내었으며, MMP-1 저해능은 25 ㎍/ml에서 20%의 활성을 나타냈다. 이 결과로 ZMF는 주름개선용 화장품 소재로 응용이 가능할 것으로 판단된다. 항산화, 항염증, 주름개선 평가 결과, 양하의 생리 활성 효과가 검증되었으므로 천연 화장품 재료로 사용될 수 있을 것으로 사료된다.

Keywords

References

  1. W. K. Whang, Y. A. Lee, H. J. An, Y. K. Jo, "Buying Attitude on Cosmetics of Women with Life Style", Kor. J. Aesthet. Cosmetol, Vol.8, No.2, pp. 117-126, (2010).
  2. Y. S. Han, E. S. Jung, "A study of correlation between antioxidant activity and whitening effect of plant extracts", Kor. J. Aesthet. Cosmetol, Vol.1, No.1, pp. 11-22, (2003).
  3. N. Asano, E. Tomioka, H. Kizu, K. Matsui, "Sugars with nitrogen in the ring isolated from the leaves of Morus bombycis", Carbohydrate Research, Vol.253, pp. 235-245, (1994). https://doi.org/10.1016/0008-6215(94)80068-5
  4. C. S. Eun, E. Y. Hwang, S. A. Yang, M. H. Yu, "Anti-oxidant and anti-inflammatory activites of Barely sprout extract", J. Life Sci, Vol.26, NO.5 pp. 537-544, (2016). https://doi.org/10.5352/JLS.2016.26.5.537
  5. Y. J. Shin, "Antioxidant Activities of Medicinal Plant Extracts", J. Kor. Soc. Food Sci. Nrtr, Vol.42, No.4, pp. 512-519, (2013). https://doi.org/10.3746/jkfn.2013.42.4.512
  6. A. J. Bailey, S. P. Robinson, G Balian, "Biological significance of the intermolecular crosslinks of collagen", Natute 251, pp. 105-109, (1974). https://doi.org/10.1038/251105a0
  7. H. Y. Kim, S. I. Y, J. T. Lee, "Antioxidant Effect of Solvent Fraction from Sanguisorbae officinalis L. with Acetone", J. Appl. Biol. Chem, Vol.54, No.2, pp 89-93, (2011). https://doi.org/10.3839/jabc.2011.016
  8. H. Lee, G. S. Jin, Y. R. Son, H. J. Kwon, B. W. Kim, "Anti-oxidative and Anti-inflammatory Activities of Desmodium heterocarpon Extract in RAW 264.7 Cells", J. Life Sci, Vol.28, NO.2, pp. 216-222, (2018). https://doi.org/10.5352/JLS.2018.28.2.216
  9. J. W. Lee, S. U. Chon, S. K. Han, D. G. Choi, J. Ryu, "Effects of antioxidant and flavor components of Zingiber Miogar Rosc", Korean J. Medicinal Crop Sci, Vol.15, pp. 203-209, (2007).
  10. M. S. Cho, "Food Materials and Use Method in Donguibogam", Youlchon org, pp. 569, (2012).
  11. D. K. Ahn, "Illustrated Book of Korean Medicinal herbs", Kyhaksa, p. 578, (2003).
  12. K. C. Jang, S. C. Kim, E. Y. Song, K. H. Kim, H. M. Kwon, S. H. Kang, K. H. Park, Y. H. Jung, "Isolation and substances from the rhizome of Zingiber Miogar Rosc", J. Korean Soc. Agric. Chem. Biotechnol., Vol.46, pp. 246-250, (2003).
  13. G. S. Jeong, S. I. Im, B. M. Jung, Comparison of nutritional constituents of native Yangha (Zingiber Miogar) in Yeosu and Cheju area. Korean J. Food Sci. Technol., Vol.37, pp. 715-716, (2005).
  14. J. H. Shin, S. J. Lee, N. J. Sung, "Effects of Zingiber Miogar root and Zingiber officinale on the lipid concentration in hyperlipidemic rats", J. Korean soc. Food Sci. Nutr, Vol.31, pp. 679-684, (2002). https://doi.org/10.3746/jkfn.2002.31.4.679
  15. M. S. Blois, "Antioxidant determination by the use of a stable free radical", Nature, Vol.181, pp. 1199-1200, (1958). https://doi.org/10.1038/1811199a0
  16. N. Fellegrini, R. Ke, M. Yang, C. Rice-Evans, "Screeniug of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2'-azinobis (3-ethylenebenzothiazoline-6-sulfonic acid) radical cation decolorization assay", Method Enzymol, Vol.299, pp. 379-389, (1999). https://doi.org/10.1016/S0076-6879(99)99037-7
  17. S. Marklund, G. Marklund,"Involvement of the superoxide anion radical in the autoxidation of pyrogallol and convenient assay for superoxide dismutase", Eur. J. Biochem, Vol.47, pp. 469-474, (1974). https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  18. J. Carmichael, W. G. DeGraff, A. F. Gazdar, J. D. Minna, J. B. Mitchell, "Evaluation of a tetrazolium based semiautomated colorimetric assay: assessment of chemosensitivity testing", Cancer Res, Vol.47, pp. 936-942, (1987).
  19. L. Marcocci, J. J. Maguire, M. T. Droylefaix, L. Packer,"The nitric oxide-scavenging properties of Ginkgo biloba extract EGb 761. Biochemical and Biophysical Research Communications", Vol.201, pp. 748-755, (1994). https://doi.org/10.1006/bbrc.1994.1764
  20. J. Gross, C. M. Lapiere, "Collagenolytic activity in amphibian tissues: a tissue culture assay", Proc Natl Acad Sci USA, Vol.54, pp. 1197-1204, (1962).
  21. M. Krupsky, A. Fine, J. L. Berk, R. H. Goldstein, "Retinoic acid-induced inhibition of type I collagen gene expression by human lung fibroblasts", Biochemica. Biophysica. Acta. Vol.1209, pp. 335-341, (1994).
  22. E. K. Park, S. R. Ahn, D. H. Kim, E. W. Lee, H. J. Kwon, B. W. Kim, T. H. Kim, "Effects of unripe apple polyphenols on the expression of matrix metalloproteinase -1 and type-1 procollagen in ultraviolet irradiated human skin fibroblasts", J. Korean Soc. Appl. Biol. Chem, Vol.57, pp. 449-455, (2014). https://doi.org/10.1007/s13765-014-4128-7
  23. W. Brand-Williams, M. E. Cuvelier, C. Berset, "Use of a free radical method to evaluate antioxidant activity", Lebensm-Wiss. u-Tech, pp. 28, 25-30, (1995). https://doi.org/10.1016/S0023-6438(95)80008-5
  24. J. E. Ancerewicz, P. A. Migliavacca, B. Carrupt, F. Testa, R. Bree, J. P. Zini, S. Tillerment, D. Labidalle, A. M. Guyot, C. A. Chauvent-Monges, A. L. Ridant, "Structure property relationships of trimetazidine derivatives and model compounds as potential antioxidants", Free Rad. Biol. Med, Vol.25, pp. 113-120, (1998). https://doi.org/10.1016/S0891-5849(98)00072-0
  25. J. S. Lee, H. H. Jang, G. Y. Song, "Changes in Quality Characteristics and Bioactivity of Rehmanniae Radix Jung Kwa Obtained by Different Heat Processing Times", Kor. J. Aesthet. Cosmetol, Vol.11, pp. 59-69, (2013).
  26. Y. D. Kim, "Physicochemical Components and Physiological Activities in Root and Aerial Parts of Zingiber Miogar R", Sunchon National University, pp. 55-57, (2017)
  27. H. J. Lee, N. W, A. J. Kim, "Antioxidant Activities and Quality Characteristics Sulgidduk Prepared with Yangha Buds (Zingiber Miogar R)", J. Kor. Soc. Food Cult., Vol.29, No.6, pp. 615-622, (2014). https://doi.org/10.7318/KJFC/2014.29.6.615
  28. R. L Prior, X. Wu, K. Schaich, "Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements", J. Agricultural and Food Chemistry, Vol.53, No.10, pp. 290-4302, (2005).
  29. R. Van den Berg, G. R. M. M. Haenen, H. Van den Berg, A. Bast, "Applicability of an improved Trolox equivalent antioxidant capacity (TEAC) assay for evaluation of antioxidant capacity measurements of mixtures", Food Chem, Vol.66, pp. 511-517, (1999). https://doi.org/10.1016/S0308-8146(99)00089-8
  30. J. V. Bannister, G. R. Bannister, "Aspects of the structure, function and applications of superoxide dismutase", J. Crit Rev Biochem, Vol.22, pp. 111-180, (1987). https://doi.org/10.3109/10409238709083738
  31. J. M. McCord, I. Fridovich, "Superoxide dismutase an enzyme funtion for erythrocuprein(Hemocuprein). J. Biol. Chem, Vol.244, No.22, p. 6049, (1969). https://doi.org/10.1016/S0021-9258(18)63504-5
  32. J. G. Park, B. S. Karmer, S. M. Steninberg, J. Carmichael, J. M. Collins, J. D. Minna, A. F. Gazdar, "Chemosensitivity testing of human colorectal carcinoma cell lines using a tetrazolium-based colorimetric assay", J. Cancer Res, Vol.47, pp. 5875-5884, (1987).
  33. T. Mosmann, "Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays", J Immunol Methods., Vol.65, pp. 55-63, (1983). https://doi.org/10.1016/0022-1759(83)90303-4
  34. S. Kwon, R. L. Newcomb, S. C. George, "Mechanisms of synergistic cytokine-induced nitric oxide production in human alveolar epithelial cells", Nitric Oxide, Vol.5, No.6 pp. 534-546, (2001). https://doi.org/10.1006/niox.2001.0387
  35. T. J. Guzik, R. Korbut, T. Adamek-Guzik. "Nitric oxide and superoxide in inflammation and immune regulation", Journal of Physiology and Pharmacology, Vol.54, No.4 pp. 469-487, (2003).
  36. J. K. Kundu, Y. J. Surh, "Emerging avenues linking inflammation and cancer", J. Free Radical Biology and Medicine, Vol.52, No.9 pp. 2013-2037, (2012). https://doi.org/10.1016/j.freeradbiomed.2012.02.035
  37. H. Nagase, J. F. Woessner, "Matrix metalloproteinases", J. Biol. Chem, Vol.274, pp. 21491-21494, (1999). https://doi.org/10.1074/jbc.274.31.21491
  38. J. M. Yoo, Y. J. Kang, H. B. Pyo, E. S. Choung, S. Y. Park, J. H. Choi, G. J. Han, C. H. Lee, T. J. Kim, "Anti-wrinkle effects of Korean rice wine cake on human fibroblast", J. Life Sci, Vol.20, pp. 1838-1843, (2010). https://doi.org/10.5352/JLS.2010.20.12.1838
  39. M. Martinez-Esparza, C. Jimenez-Cervantes, F. Solano, J. A. Lozano, J. C. Garcia-Borron, "Mechanisms of melanogenesis inhibition by tumor necrosis factor-alpha in B16/F10 mouse melanoma cells", Eur. J. Biochem, Vol.255, No.27 pp. 139-146, (1998). https://doi.org/10.1046/j.1432-1327.1998.2550139.x

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