The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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Anti-oxidant and Anti-aging Effect of Extracts from Seed of Camellia japonica L. by Supercritical Fluid Extraction

한국산 동백종자 초임계 추출물의 피부 항산화 및 항노화 연구

  • Kim, Hee-Taek (Dept. Of Korean Medical Ophtalmology & Otolaryngology & Dermatology, College of Korean Medicine, Semyung University) ;
  • Kim, Tae-Jun (Dept. Of Korean Medical Ophtalmology & Otolaryngology & Dermatology, College of Korean Medicine, Semyung University) ;
  • Kim, Ee-Hwa (Dept. Of Meridian and Acupoint, College of Korean Medicine, Semyung University) ;
  • Kwak, Byeong-Mun (Dept. Of Meridian and Acupoint, College of Korean Medicine, Semyung University)
  • 김희택 (세명대학교 한의과대학 한방안이비인후피부과) ;
  • 김태준 (세명대학교 한의과대학 한방안이비인후피부과) ;
  • 김이화 (세명대학교 한의과대학 경락경혈학교실) ;
  • 곽병문 (세명대학교 한의과대학 경락경혈학교실)
  • Received : 2020.10.03
  • Accepted : 2020.10.31
  • Published : 2020.11.25
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Abstract

Objectives : The aim of this study was to investigate the anti-oxidant and anti-aging effect of the seed of Camellia japonica L. extracted by supercritical CO2. Methods : The cell viability was performed by MTT assay. Nitric oxide (NO) production was performed by NO assay. The anti-oxidant effect was performed by beta-carotene bleaching assay and the intracellular proteome was analyzed expression of each 15 proteins by 2-D electrophoresis. Results : Fatty acid analysis of extract from Camellia japonica L. has shown oleic acid was 84%. And the antioxidant effect was about 186% compared with alpha-tocoperol(0.1%) by beta carotene bleaching assay. In 2D PAGE analysis, fifteen protein changes in five mechanisms which was collagen synthesis pathway, MMPs, ECM-cell interaction, cytokine, antioxidant enzymes were analyzed. Conclusions : On this study extract from seed of Camellia japonica L. by supercritical extraction could be used as a antioxidant and antiaging substance for the skin.

Keywords

References

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