Journal of Species Research

The National Institute of Biological Resources (국립생물자원관)
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Twelve unrecorded UV-resistant bacterial species isolated in 2020

  • Kim, Ju-Young (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Maeng, Soohyun (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Park, Yuna (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Lee, Sang Eun (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Han, Joo Hyun (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University) ;
  • Cha, In-Tae (National Institute of Biological Resources) ;
  • Lee, Ki-eun (National Institute of Biological Resources) ;
  • Kim, Myung Kyum (Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University)
  • Received : 2021.01.26
  • Accepted : 2021.07.13
  • Published : 2021.11.30
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Abstract

In 2020, a total of 12 bacterial strains were isolated from soil after a comprehensive investigation of indigenous prokaryotic species in Korea. It was determined that each strain belonged to independent and predefined bacterial species, with high 16S rRNA gene sequence similarity (>98.7%) and formation of a robust phylogenetic clade with the closest species. This study identified four families in the phylum Actinobacteria, two families in the phylum Proteobacteria, one family in the phylum Bacteroidetes one family in the phylum Firmicutes; and four species in the family Nocardiaceae, two species in the family Nocardioidaceae, one species in the family Cellulomonadaceae, one species in the family Hymenobacter, one species in the family Methylobacteriaceae, one species in the family Microbacteriaceae, one species in the family Bacillaceae and one species in the family Sphingomonadaceae. There is no official report of these 12 species in Korea, so they are described as unreported bacterial species in Korea in this study. Gram reaction, basic biochemical characteristics, colony, and cell morphology are included in the species description section.

Keywords

Acknowledgement

This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR202002108). This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2021R1F1A1061389).

References

  1. Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783-791. https://doi.org/10.2307/2408678
  2. Gao, B. and R.S. Gupta. 2012. Phylogenetic Framework and Molecular Signatures for the Main Clades of the Phylum Actinobacteria. Microbiol. Mol. Biol. Rev. 76:66-112. https://doi.org/10.1128/MMBR.05011-11
  3. Gupta, R.S. 2000a. The natural evolutionary relationships among Prokaryotes. Journal of Critical Reviews in Microbiology 26(2):111-131. https://doi.org/10.1080/10408410091154219
  4. Gupta, R.S. 2000b. The phylogeny of proteobacteria: relationships to other eubacterial phyla and eukaryotes. FEMS Microbiology Review 24(4):367-402. https://doi.org/10.1016/S0168-6445(00)00031-0
  5. Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. In: Nucleic acids symposium series Information Retrieval Ltd, London.
  6. Im, S., Song, D., Joe, M., Kim, D., Park, D.H., Lim, S.(2013) Comparative survival analysis of 12 histidine kinase mutants of Deinococcus radiodurans after exposure to DNA-damaging agents. Bioproc. Biosyst. Eng. 36:781-789. https://doi.org/10.1007/s00449-013-0904-8
  7. Kimura, M. 1983. The neutral theory of molecular evolution. Cambridge University Press.
  8. Kumar, S., G. Stecher and K. Tamura. 2016. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datadets. Molecular Biology and Evolution 33:1870-1874. https://doi.org/10.1093/molbev/msw054
  9. Ludwig, W., J. Euzeby and W.B. Whitman. 2010. Road map of the phyla Bacteroidetes, Spirochaetes, Tenericutes (Mollicutes), Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemmatimonadetes, Lentisphaerae, Verrucomicrobia, Chlamydiae, and Planctomycetes. Bergey's Manual of Systematic Bacteriology vol. 4, Springer, pp. 1-19.
  10. Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406-425.
  11. Selvam, K., J.R. Duncan, M. Tanaka and J.R. Battista. 2013. DdrA, DdrD, and PprA: components of UV and mitomycin C resistance in Deinococcus radiodurans R1. PLoS One 8(7):e69007. https://doi.org/10.1371/journal.pone.0069007
  12. Weisburg, W.G., S.M. Barns, D.A. Pelletier and D.J. Lane. 1991. 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology 173:697-703. https://doi.org/10.1128/jb.173.2.697-703.1991
  13. Wragg, P., L. Randall and A.M. Whatmore. 2014. Comparison of Biolog GEN III MicroStation semi-automated bacterial identification system with matrix-assisted laser desorption ionization-time of flightmass spectrometry and 16S ribosomal RNA gene sequencing for the identification of bacteria of veterinary interest. Journal of Microbiological Methods 105:16-21. https://doi.org/10.1016/j.mimet.2014.07.003
  14. Yoon, S.H., S.M. Ha, S. Kwon, J. Lim, Y. Kim, H. Seo and J. Chun. 2017. Introducing EzBioCloud: A taxonomically united database of 16S rRNA and whole genome assemblies. Int. J. Syst. Evol. Microbiol. 67:1613-1617. https://doi.org/10.1099/ijsem.0.001755