The Journal of Pediatrics of Korean Medicine (대한한방소아과학회지)

The Association of Korean Oriental Pediatrics (대한한방소아과학회)
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Inflammation Relief Effect through ECS Control of Galgeunhwanggeumhwangryeon-tang Extract in Atopic Dermatitis

아토피피부염에서 갈근황금황련탕 추출물의 ECS 조절을 통한 염증 완화 효과

  • Kim, Ki Bong (School of Korean Medicine, Pusan National University) ;
  • Ahn, Sang Hyun (Dept. of anatomy, college of Korean Medicine, Semyung University)
  • 김기봉 (부산대학교 한의학전문대학원 한방소아과학교실) ;
  • 안상현 (세명대학교 한의과대학 해부학교실)
  • Received : 2021.10.17
  • Accepted : 2021.11.18
  • Published : 2021.11.30
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Abstract

Objective The purpose of this study was to confirm the effects of Galgeunhwanggeumhwangryeon-tang in reducing inflammation through the endocannabinoid system (ECS) control in atopic dermatitis. Methods 8-week-old Balb/C mice were divided into 4 groups: contorl group (Ctrl), lipid barrier elimination group (ADE), palmitoylethanolamide treated group after lipid barrier elimination (PEA), and Galgeunhwanggeumhwangryeon-tang applied group after lipid barrier elimination (GGRT). After inducing atopic dermatitis, cannabinoid receptor (CB) 1, CB2, CD68, phosphorylated inhibitor kappa B (p-IκB), inducible nitric oxide synthase (iNOS), substance P and serotonin were observed to confirm the regulation of the ECS, macrophage activity and mast cell activity. Results CB1 and CB2 showed higher positive reactions in the GGRT than in the LBE and PEA. CD68, p-IκB and iNOS showed higher positive reaction in the LBE, PEA and GGRT than in the Ctrl, but the increase in the positive reaction was lower in the GGRT compared to the LBE and PEA. Substance P and serotonin showed higher positive reaction in the LBE, PEA and GGRT than in the Ctrl, but the increase in the positive reaction was lower in the GGRT compared to the LBE and PEA. Conclusions The effects of Galgeunhwanggeumhwangryeon-tang were confirmed though the regulation of the ECS, macrophage activity and mast cell activity.

Keywords

Acknowledgement

이 논문은 2021학년도 세명대학교 교내학술연구비 지원에 의해 수행된 연구임.

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