Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Hepatoprotective effect of Ikwiseungyang-tang via Nrf2 activation

Nrf2 활성화를 통한 익위승양탕(益胃升陽湯)의 간세포 보호 효과

  • Jin, Hyo Jeong (College of Korean Medicine, Daegu Haany University) ;
  • Park, Sang Mi (College of Korean Medicine, Daegu Haany University) ;
  • Kim, Eun Ok (College of Korean Medicine, Daegu Haany University) ;
  • Kim, Sang Chan (College of Korean Medicine, Daegu Haany University)
  • 진효정 (대구한의대학교 한의과대학 방제학교실) ;
  • 박상미 (대구한의대학교 한의과대학 방제학교실) ;
  • 김은옥 (대구한의대학교 한의과대학 방제학교실) ;
  • 김상찬 (대구한의대학교 한의과대학 방제학교실)
  • Received : 2021.09.03
  • Accepted : 2021.10.15
  • Published : 2021.11.30
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Abstract

Objectives : Oxidative stress is a important cause of liver disease, and regulation of oxidative stress is essential to maintain the normal metabolic function of the liver. Until a recent date, there has been no studies on the hepatoprotective effect of Ikwiseungyang-tang (IWSYT). Therefore, this study aims to demonstrate the hepatoprotective effect of IWSYT and its related molecular mechanisms on arachidonic acid (AA) + iron induced oxidative stress model in HepG2 cells. Methods : To determine the cytoprotective effect of IWSYT against AA + iron-induced oxidative stress, cell viability, apoptosis-related proteins, intracellular reactive oxygen species (ROS), GSH, and mitochondrial membrane potential (MMP) were measured. Nuclear factor erythroid 2-related factor 2 (Nrf2) activation was analyzed by immunoblot analysis. In addition, Nrf2 transcription activation through ARE binding was measured by reporter gene assays, and the expression of the Nrf2 target antioxidant genes were confirmed by immunoblot analysis. Results : IWSYT increased cell viability from cell death induced by AA + Iron, and inhibited apoptosis by regulating apoptosis-related proteins. Furthermore, IWSYT protected cells by inhibiting intracellular ROS production, GSH depletion, and MMP degradation. Nrf2 activation was increased by IWSYT, and Nrf2 target genes were activated by IWSYT too. Conclusions : These results suggest that IWSYT can protect hepatocytes from oxidative stress through Nrf2 activation and can be potentially applied in the prevention and treatment of liver damage.

Keywords

Acknowledgement

This study was supported by the National Research Foundation of Korea funded by Korea government (MSIP) (Grant No.2018R1A5A2025272).

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