Journal of Convergence for Information Technology (융합정보논문지)

Convergence Society for SMB (중소기업융합학회)
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A Study on Anti-oxidant and Anti-wrinkle Effect of Supercritical Fluid Extraction of Black Carrot as a Functional Cosmetic Material

기능성화장품소재로서 자색당근 초임계추출물의 항산화 및 항주름 효능 연구

  • Kim, Ji-Su (Dept. of Beauty Art, Graduate School, Seokyeong University) ;
  • Lee, Ji-An (Dept. of Beauty Therapy & Make-up, College of Beauty Art, Seokyeong University)
  • 김지수 (서경대학교 일반대학원 미용예술학과) ;
  • 이지안 (서경대학교 미용예술대학 뷰티테라피&메이크업학과)
  • Received : 2021.10.03
  • Accepted : 2021.12.20
  • Published : 2021.12.28
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Abstract

The aim of this study was to evaluate the anti-oxidant and anti-wrinkle effect of the black carrot(BC) extracted by supercritical dioxide(SC-CO2). DPPH/ABTS radical scavenging and total polyphenol contents were measured to investigate the anti-oxidant activity of the BC supercritical extract. Collagen production and MMP-1 expression were assessed in normal human dermal fibroblasts(NHDF) for anti-wrinkle activity, The black carrot extract showed the highest total phenolic content(9.037±1.123 mg GAE/g extract) and the best antioxidant properties as shown by DPPH and ABTS assay. The supercritical fluid extracts of black carrot exhibited low toxicity to NHDF cells. In addition, the supercritical fluid extracts showed MMP-1 inhibition and type I pro-collagen synthesis inducing activities in a dose-dependent manner, respectively. Therefore, these results suggest that the black carrot is a potential source of high anti-oxidative, solvent-free and anti-aging material with promising applications in cosmetic, food, and beauty-food industries.

본 연구는 자색당근(black carrot, BC) 소재로 초임계추출물에 대해 DPPH radical 소거능, ABTS radical 소거능, 총 폴리페놀 함량 측정을 통해 항산화 활성, 인간의 정상 섬유아세포에서 콜라겐 생성과 MMP-1 발현을 조사하여 항주름 효능을 평가하고자 하였다. 그 결과 자색당근 초임계추출물 내 총 폴리페놀 함량 (9.037±1.123 mg GAE/g), DPPH, ABTS 분석 결과로 높은 항산화 효과를 확인하였다. NHDF 세포에서 초임계추출물 농도에 의한 세포독성은 거의 없었으며, 이때 추출물은 농도 의존적으로 MMP-1 단백질 및 MMP-1 mRNA 발현을 억제하고, 콜라겐 type I 유전자 mRNA 수준 증가에 의한 type I procollagen의 합성을 촉진하였다. 이상의 결과를 종합해 볼 때, 자색당근 초임계추출물은 우수한 항산화 효능과 친환경(solvent free)물질, 항노화 원료로서 화장품, 식품, 미용식품 등 다양한 산업분야에 있어 활용 가능성이 매우 높을 것으로 판단된다.

Keywords

Acknowledgement

This Research was supported by Seokyeong University in 2021.

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