Journal of Applied Biological Chemistry

  • 제64권4호
  • /
  • Pages.447-451
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  • 2021
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  • 1976-0442(pISSN)
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  • 2234-7941(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
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Linarin enhances melanogenesis in B16F10 cells via MAPK and PI3K/AKT signaling pathways

  • Oh, So-Yeon (Jeju Inside Agency and Cosmetic Science Center, Department of Chemistry and Cosmetics, Jeju National University) ;
  • Kang, Jin Kyu (Jeju Inside Agency and Cosmetic Science Center, Department of Chemistry and Cosmetics, Jeju National University) ;
  • Hyun, Chang-Gu (Jeju Inside Agency and Cosmetic Science Center, Department of Chemistry and Cosmetics, Jeju National University)
  • 투고 : 2021.10.24
  • 심사 : 2021.11.16
  • 발행 : 2021.12.31
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초록

In this study, we discovered for the first time that linarin, a flavonoid compound, enhances melanin biosynthesis in B16F10 cells, and subsequently elucidated the underlying mechanism of linarin-induced melanogenesis. Linarin showed no cytotoxicity at a concentration of 42 μM and significantly increased intracellular tyrosinase activity and melanin content in B16F10 cells. Mechanistic analysis showed that linarin increased the expression of tyrosinase, tyrosinase-related protein 1 (TRP-1), and microphthalmia-associated transcription factor (MITF) that are related to melanogenesis. Moreover, linarin decreased the phosphorylation of extracellular signal-regulated kinase (ERK) and protein kinase B (AKT). Finally, we evaluated the effect of the structure-activity relationship of linarin and its aglycone on melanogenesis. The results indicated that linarin enhances the expression of melanogenic proteins by activating MITF expression via the modulation of mitogen-activated protein kinase (MAPK), phosphatidylinositol 3-kinase (PI3K), and protein kinase B signaling pathways in B16F10 cells, thereby enhancing melanogenesis.

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과제정보

This research was supported by the Ministry of Trade, Industry & Energy (MOTIE), Korea Institute for Advancement of Technology (KIAT) through the Industry Innovation Foundation Project (P0018139).

참고문헌

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