한방재활의학과학회지 (Journal of Korean Medicine Rehabilitation)

  • 제31권1호
  • /
  • Pages.59-79
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  • 2021
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  • 1229-1854(pISSN)
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  • 2288-4114(eISSN)
한방재활의학과학회 (The society of Korean Medicine Rehabilitation)
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ChondroT의 유전독성 연구

Genotoxicity Study of ChondroT

  • 김선길 (동신대학교 한의과대학 한방재활의학과교실) ;
  • 김주일 (동신대학교 한의과대학 한방재활의학과교실) ;
  • 김지훈 (동신대학교 한의과대학 한방재활의학과교실) ;
  • 윤찬석 (동신대학교 한의과대학 한방재활의학과교실) ;
  • 정지원 (동신대학교 한의과대학 한방재활의학과교실) ;
  • 나창수 (동신대학교 한의과대학 경락경혈학회) ;
  • 김선종 (동신대학교 한의과대학 한방재활의학과교실)
  • Kim, Sun-Gil (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongshin University) ;
  • Kim, Joo Il (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongshin University) ;
  • Kim, Ji-Hoon (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongshin University) ;
  • Yoon, Chan Suk (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongshin University) ;
  • Jeong, Ji-Won (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongshin University) ;
  • Na, Chang-Su (Department of Meridian and Acupoint, College of Korean Medicine, Dongshin University) ;
  • Kim, Seon-Jong (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Dongshin University)
  • 투고 : 2020.09.15
  • 심사 : 2020.09.21
  • 발행 : 2021.01.31
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초록

Objectives This study was performed to observe the genotoxic effect of the ChondroT. Methods To evaluate the genotoxicity of ChondroT, an experiment of bacterial reverse mutation test, in vitro mammalian chromosomal aberration test and mammalian erythrocyte micronucleus test in mouse was conducted. Results TA98, TA100 and TA1537 strains in the absence of metabolic activation system (S9 mix), the number of revertant colonies being greater than 2-fold of the respective negative control value. Both in -S9 mix and +S9 mix, the frequencies of aberration cells with structural aberration and numerical aberrations of chromosome were less than 5%. There was no increase of polychromatic erythrocyte with one or more micronuclei at any dose of test substance compared to the negative control group (p<0.05). Conclusions In TA98, TA100 and TA1537 strains in the absence of metabolic activation system (S9 mix), the number of revertant colonies was greater than 2-fold of the respective negative control value, showing positive results. ChondroT was considered to be non-clastogenic to Chinese hamster lung (CHL/IU) cells under the present experimental condition. and ChondroT was determined not to induce an increased frequency of micronuclei in the bone marrow cells of male ICR mice under the present experimental condition.

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참고문헌

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