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A Study on the β-glucan, Ginsenoside Content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) Free Radical Scavenging Activity and Single Dose Toxicity Assessment of Modified Kyungohkgo

경옥고가미방의 베타글루칸, 진세노사이드 함량, 2,2-diphenyl-1-picrylhydrazyl (DPPH) Free Radical 소거 활성 및 단회 투여 독성 연구

  • Lee, Yu-Mi (Department of Meridian & Acupoint.Diagnosis, College of Korean Medicine, Dongshin University) ;
  • Moon, Yang-Seon (Nawoori (Ltd)) ;
  • Park, Hee-Myeong (Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University) ;
  • Kim, Heyong-Seok (Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University) ;
  • Ro, Woong-Bin (Department of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University) ;
  • Na, Chang-Su (Department of Meridian & Acupoint.Diagnosis, College of Korean Medicine, Dongshin University)
  • 이유미 (동신대학교 한의과대학 경혈진단학교실) ;
  • 문양선 ((유)나우리) ;
  • 박희명 (건국대학교 수의과대학 수의내과학교실) ;
  • 김형석 (건국대학교 수의과대학 수의내과학교실) ;
  • 노웅빈 (건국대학교 수의과대학 수의내과학교실) ;
  • 나창수 (동신대학교 한의과대학 경혈진단학교실)
  • Received : 2020.12.22
  • Accepted : 2020.12.30
  • Published : 2021.01.31

Abstract

Objectives This study was conducted to investigate the beta-glucan, ginsenoside content, antioxidant activity and safety of modified Kyungohkgo added to Sparassis crispa and Hericium erinaceum. Methods The marker compounds contents, antioxidant activity and safety of modified Kyungohkgo were tested. The contents of beta-glucan and ginsenoside Rb1, Rg1, and Rg3 marker compounds were measured, the antioxidant activity was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, and a safety test was conducted via single dose toxicity assessment. Results Analyzing the contents of marker compounds showed 351.75 mg/g of beta-glucan, 0.0327 mg/g of ginsenoside Rb1 and 0.0802 mg/g of ginsenosai Rg3. In the DPPH free radical scavenging activity, the inhibition concentration 50% of modified Kyungohkgo was 0.2880%. The scavenging activity of modified Kyungohkgo was 5.49% activity at 0.05% concentration, 89.66% activity at 0.5% concentration, 94.68% activity at 1% concentration, and 96.06% activity at 5% concentration. In the single dose toxicity test of modified Kyungohkgo, a dose of 2,000 mg/kg B.W. was set at its highest capacity and observed after oral administration to female and male rats. No toxicological findings were recognized. It was observed that the resulting lethal dose can be set to 2,000 mg/kg B.W. or higher for both females and males. Conclusions The results of the experiment on modified Kyungohkgo showed that the marker compounds contents were beta-glucan and ginsenoside Rb1 and Rg3, that antioxidant activity was observed through the DPPH free radical scavenging activity, and safety was confirmed through the single dose toxicity assessment.

Keywords

Acknowledgement

This paper has been written with the support of Jeollannam-do('2019 R&D supporting program' operated by Jeonnam Technopark.

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