Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Actinidia arguta Sprout as a Natural Antioxidant: Ameliorating Effect on Lipopolysaccharide-Induced Cognitive Impairment

  • Kang, Jeong Eun (Division of Applied Life Science, Institute of Agriculture and Life Science (BK21), Gyeongsang National University) ;
  • Park, Seon Kyeong (Division of Applied Life Science, Institute of Agriculture and Life Science (BK21), Gyeongsang National University) ;
  • Kang, Jin Yong (Division of Applied Life Science, Institute of Agriculture and Life Science (BK21), Gyeongsang National University) ;
  • Kim, Jong Min (Division of Applied Life Science, Institute of Agriculture and Life Science (BK21), Gyeongsang National University) ;
  • Kwon, Bong Seok (Division of Applied Life Science, Institute of Agriculture and Life Science (BK21), Gyeongsang National University) ;
  • Park, Sang Hyun (Division of Applied Life Science, Institute of Agriculture and Life Science (BK21), Gyeongsang National University) ;
  • Lee, Chang Jun (Division of Applied Life Science, Institute of Agriculture and Life Science (BK21), Gyeongsang National University) ;
  • Yoo, Seul Ki (Division of Applied Life Science, Institute of Agriculture and Life Science (BK21), Gyeongsang National University) ;
  • Heo, Ho Jin (Division of Applied Life Science, Institute of Agriculture and Life Science (BK21), Gyeongsang National University)
  • Received : 2020.09.08
  • Accepted : 2020.10.07
  • Published : 2021.01.28
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Abstract

Here, we investigated the prebiotic and antioxidant effects of Actinidia arguta sprout water extract (AASWE) on lipopolysaccharide (LPS)-induced cognitive deficit mice. AASWE increased viable cell count, titratable acidity, and acetic acid production in Lactobacillus reuteri strain and showed a cytoprotective effect on LPS-induced inflammation in HT-29 cells. We assessed the behavior of LPS-induced cognitive deficit mice using Y-maze, passive avoidance and Morris water maze tests and found that administration of AASWE significantly improved learning and memory function. The AASWE group showed antioxidant activity through downregulation of malondialdehyde levels and upregulation of superoxide dismutase levels in brain tissue. In addition, the AASWE group exhibited activation of the cholinergic system with decreased acetylcholinesterase activity in brain tissue. Furthermore, AASWE effectively downregulated inflammatory mediators such as phosphorylated-JNK, phosphorylated-NF-κB, TNF-α and interleukin-6. The major bioactive compounds of AASWE were identified as quercetin-3-O-arabinopyranosyl(1→2)-rhamnopyranosyl(1→6)-glucopyranose, quercetin-3-O-apiosyl(1 → 2)-galactoside, rutin, and 3-caffeoylquinic acid. Based on these results, we suggest that AASWE not only increases the growth of beneficial bacteria in the intestines, but also shows an ameliorating effect on LPS-induced cognitive impairment.

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