한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제49권4호
  • /
  • Pages.566-575
  • /
  • 2021
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  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Production of α-Glucosidase Inhibitor and 1-Deoxynojirimycin by Bacillus subtilis MORI

  • Park, Young Shik (Department of Biotechnology and Biomarketing, The University of Suwon) ;
  • Lee, Jae Yeon (Department of Biotechnology and Biomarketing, The University of Suwon) ;
  • Hwang, Kyo Yeol (Department of Biotechnology and Biomarketing, The University of Suwon) ;
  • Kim, Keun (Department of Biotechnology and Biomarketing, The University of Suwon)
  • 투고 : 2021.11.15
  • 심사 : 2021.12.10
  • 발행 : 2021.12.28
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초록

Galactose and soybean meal were selected as the best carbon and nitrogen sources, repectively, for the efficient production of α-glucosidase inhibitor (AGI) and 1-deoxynojirimycin (DNJ) by a newly isolated Bacillus subtilis MORI. The optimal concentrations of the galactose and soybean meal for the production of AGI and DNJ were investigated by response surface methodology. For the production of AGI, the optimal galactose and soybean meal concentrations were 4.3% (w/v) and 3.2% (w/v), respectively, and for DNJ, 4.5% (w/v) and 3.0% (w/v), respectively. The nearly identical optimal concentrations of galactose and soybean meal for the production of both AGI and DNJ indicated a close correlation between the production of AGI and DNJ. The maximum production of AGI (50,880 GIU/ml) and DNJ (824 ㎍/ml) obtained from the statistically optimized medium in a jar fermenter was 2.33 and 2.38-fold, respectively, higher than those (21,798 GIU/ml and 346 ㎍/ml, respectively) of the pre-optimized medium. The production of both AGI and DNJ was greatly increased by jar fermentation (AGI of 38,524 GIU/ml and DNJ of 491 ㎍/ml) compared with flask fermentation.

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