Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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Effects of Inhibiting Glycoprotein MUC5AC by Seaweed Ecklonia cava Extract in human Airway Epithelial Cells

  • Lee, Sung-Gyu (Department of Medical Laboratory Science, College of Health Science, Dankook University) ;
  • Kwon, Sang-Oh (Department of Medical Laboratory Science, College of Health Science, Dankook University)
  • Received : 2021.11.09
  • Accepted : 2021.12.10
  • Published : 2021.12.31
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Abstract

In the present study, antioxidant and MUC5AC mucin inhibition activities were measured in Ecklonia cava (E. cava) extract. The E. cava extract showed the total polyphenol and flavonoid contents of 607.40±19.44 ㎍ GAE/mg and 13.33±5.28 ㎍ QE/mg, respectively. The free radical scavenging activity of E. cava extract was high in the DPPH radical scavenging activity (RC50 7.08 ㎍/mL) and ABTS+ radical scavenging activity (RC50 4.74 ㎍/mL). Also, we investigated whether E. cava extract affects airway MUC5AC mucin gene expression, production and secretion induced by phorbol 12-myristate 13-acetate (PMA) from NCI-H292 cells. Cells were treated with E. cava extract and then stimulated with PMA for 24 h. The E. cava extract inhibited the gene expression of MUC5AC mucin from NCI-H292 cells. This result suggests that E. cava extract can inhibit the gene expression of mucin induced by PMA through directly acting on airway epithelial cells.

Keywords

Acknowledgement

This research was a part of the project titled 'Development and commercialization of functional ingredient for improving respiratory diseases using diarsenic seaweed', funded by the Ministry of Oceans and Fisheries, Korea.

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