Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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Anti-inflammatory Effect of the Extract of Gynostemma pentaphyllum cell from Ullengdo Island as Korean Endemic Plant

한국 고유 식물 울릉도 돌외 식물 세포 배양추출물의 항염증 효과

  • Mok, Bo-Ram (Department of Dermatology, Bundang CHA Medical Center, CHA University School of Medicine) ;
  • Kim, Soo-Yun (Anti-aging Research Institute of BIO-FD&C Co.,Ltd.) ;
  • Paek, Seung hye (Anti-aging Research Institute of BIO-FD&C Co.,Ltd.) ;
  • Jang, Young-su (Department of Dermatology, Bundang CHA Medical Center, CHA University School of Medicine) ;
  • Shin, Jung U (Department of Dermatology, Bundang CHA Medical Center, CHA University School of Medicine) ;
  • Moh, Sang Hyun (Anti-aging Research Institute of BIO-FD&C Co.,Ltd.)
  • 목보람 (CHA의과대학교 분당차병원 피부과학교실) ;
  • 김수윤 ((주)바이오에프디엔씨 안티에이징연구소) ;
  • 백승혜 ((주)바이오에프디엔씨 안티에이징연구소) ;
  • 장영수 (CHA의과대학교 분당차병원 피부과학교실) ;
  • 신정우 (CHA의과대학교 분당차병원 피부과학교실) ;
  • 모상현 ((주)바이오에프디엔씨 안티에이징연구소)
  • Received : 2020.11.03
  • Accepted : 2021.02.05
  • Published : 2021.02.28
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Abstract

The purpose of this study was to evaluate and verify the effectiveness of sustainable cosmetic raw materials developed from Gynostemma pentaphyllum, a plant native to Ulleungdo, in improving the skin barrier function and treating atopic dermatitis. Cells were derived from adult Gynostemma pentaphyllum plants, and suitable conditions for mass culture of the cells were established in a bioreactor. DNA components and amino acids extracted from this mass culture were identified from the HPLC fraction. In the in vitro efficacy evaluation results, changes in the expression levels of skin barrier-related proteins such as filaggrin (FLG) and Zonula occludens-1 (Zo-1) were insignificant. It was confirmed that the expression levels of the proteins thymic stromal lymphopoietin (TSLP) and interleukin-33 (IL-33) were significantly reduced. These results lead to the conclusion that Gynostemma pentaphyllum cell extracts have significant anti-inflammatory effects and that these extracts can be widely used as sustainable, nature-friendly active material in cosmetics with anti-inflammatory effects and targeted at improving atopic dermatitis.They may find use in anti-aging cosmetic products as well.

이 논문의 목적은 울릉도 자생식물인 돌외식물(GP: Gynostemma pentaphyllum)을 이용하여 지속가능한 화장품 원료 개발을 위해 피부장벽개선 및 아토피피부염 개선 효능을 평가하고 검증하는 데 있다. 자연을 훼손하지 않으며 지속가능한 항노화 소재개발을 위하여 울릉도 자생식물인 돌외에서 식물세포를 유도하여 대량배양 조건을 확립, 대량배양된 식물세포로부터 다양한 용매로 추출 후, HPLC 분석을 통하여 adenosine, guanosine 및 tyrosine, phenylalanine 변화를 확인하였다. 또한, 돌외 식물세포의 피부장벽개선효능 및 항가려움증 효능평가를 위해 Th2 사이토카인을 이용한 in vitro 염증 모델에서 피부장벽관련 인자인 FLG, Zo-1의 유전자 발현에 유의미한 변화가 확인이 되지 않았지만 항가려움증 관련 인자인 TSLP, IL-33 의 유전자 발현에 유의미한 감소 변화를 확인하였다. 따라서 돌외 식물세포 추출물이 가려움증을 개선시키는 데 유의미한 효능이 있을 것으로 확인되며, 나아가 돌외 식물세포 추출물이 지속가능한 자연친화적 활성 소재로써, 아토피피부염 개선을 위한 화장품에 널리 활용될 수 있을 것이라 사료된다.

Keywords

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