The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Identification of Pseudocercospora bolleana Associated with Angular Leaf Spot on Common Fig in Korea

무화과나무의 모무늬잎마름 증상에 관여하는 Pseudocercospora bolleana 동정

  • Choi, In-Young (Department of Agricultural Biology, Jeonbuk National University) ;
  • Choi, Young-Joon (Department of Biology, Kunsan National University) ;
  • Lee, Chong-Kyu (Department of Forest Resources, Gyeongsang National University) ;
  • Shin, Hyeon-Dong (Division of Environmental Science and Ecological Engineering, Korea University)
  • 최인영 (전북대학교 농생물학과) ;
  • 최영준 (군산대학교 생물학과) ;
  • 이총규 (경상대학교 산림자원학과) ;
  • 신현동 (고려대학교 환경생태공학부)
  • Received : 2021.09.02
  • Accepted : 2021.12.01
  • Published : 2021.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

A cercosporoid fungus associated with angular leaf spots on the leaves of common fig (Ficus carica) in Korea is known to be morphologically similar to Passalora, but phylogenetically similar to Pseudocercospora. To clarify the ambiguity, six fig samples with angular leaf spots were collected and examined using a microscope, and two representative isolates were sequenced for multiple genes. The morphological characteristics were consistent with previous descriptions of Passalora bolleana. Molecular phylogenetic analysis based on the internal transcribed spacer and large subunit ribosomal DNA (rDNA) regions showed that the Korean isolates, as well as previously published Korean and Romanian isolates, formed a well-supported group in the clade of Pseudocercospora species. Consequently, the current Korean isolates should be correctly described as Pseudocercospora bolleana. Additionally, Pseudocercospora fici-caricae, a cercosporoid fungus previously described as a leaf pathogen on common fig in Taiwan and Korea, was also compared and discussed.

한국의 무화과나무 모무늬잎마름 증상에 관여하는 한 균주의 Cercospora류 곰팡이가 형태적으로는 Passalora속에 속하지만 계통학적으로는 Pseudocercospora속에 속한다고 알려져 있다. 이 문제를 해결하기 위하여, 6점의 시료를 현미경으로 검경하였으며 두 균주를 염기서열 분석하였다. 그 결과, 이들 시료의 곰팡이는 형태적으로는Passalora bolleana와 일치하였다. ITS와 LSU 염기서열을 분자계통학적으로 분석한 결과 이들 두 균주가 Pseudocercospora속 집락에 위치하였다. 결과적으로, 한국 유래의 세 균주(본 연구의 두 균주와 이전의 한 균주)와 루마니아 유래의 한 균주는 모두 Pseucocercospora bolleana로 동정되었다. 추가적으로, 대만과 한국에서 무화과나무 점무늬병균으로 알려진 Pseudocercospora fici-caricae와도 비교하고 토의하였다.

Keywords

Acknowledgement

This study was carried out with the support of the "Cooperative Research Program for Agricultural Science & Technology Development (Project Nos. PJ014976)" Rural Development Administration, Republic of Korea.

References

  1. Food and Agriculture Organization of the United Nations. Raw fig production in 2018, Crops/World Regions/Production Quantity from pick lists, UN Food and Agriculture Organization Corporate Statistical Database (FAOSTAT) [Internet], Rome: Food and Agriculture Organization of the United Nations; 2019 [cited 2021 Sept 1]. Available from: http://www.fao.org/faostat/en/#search/common%20fig.
  2. Korean Statistical Information Service (KOSIS). Statistical annual report [Internet]. Daejeon: Korean Statistical Information Service; 2015 [cited 2021 Sept 1]. Available from: https://kosis.kr/search/search.do.
  3. Farr DF, Rossman AY. Fungal databases, U.S. National Fungus Collections [Internet]. Beltsville: Systematic Mycology and Microbiology Laboratory; 2021 [cited 2021 Aug 13]. Available from http://nt.ars-grin.gov/fungaldatabases.
  4. The Korean Society of Plant Pathology. List of plant diseases in Korea. 5th ed. Seoul: Korean Society of Plant Pathology; 2009.
  5. Videira SIR, Groenewald JZ, Nakashima C, Braun U, Barreto RW, de Wit PJGM, Crous PW. Mycosphaerellaceae - chaos or clarity? Stud Mycol 2017;87:257-421. https://doi.org/10.1016/j.simyco.2017.09.003
  6. Singh R, Kumar S, Saini DC, Upadhyaya PP, Kamal S, Braun U. Diversity of Passalora on Ficus. Mycol Prog 2013;12:637-43. https://doi.org/10.1007/s11557-012-0870-6
  7. Crous PW, Braun U. Mycosphaerella and its anamorphs: 1. Names published in Cercospora and Passalora. In: Utrecht: Centraalbureau voor Schimmelcultures (CBS); 2003. 1-571.
  8. Guo Y, Hsieh W. The genus Pseudocercospora in China. Mycosystema monographicum series no. 2. Beijing: International Academic Publishers; 1995.
  9. Groenewald JZ, Nakashima C, Nishikawa J, Shin HD, Park JH, Jama AN, Groenewald M, Braun U, Crous PW. Species concepts in Cercospora: spotting the weeds among the roses. Stud Mycol 2013;75:115-70. https://doi.org/10.3114/sim0012
  10. Verkley GJM, Quaedvlieg W, Shin HD, Crous PW. A new approach to species delimitation in Septoria. Stud Mycol 2013;75:213-305. https://doi.org/10.3114/sim0018
  11. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013;30:2725-9. https://doi.org/10.1093/molbev/mst197
  12. Ryu HY, Lee YH, Cho WD, Kim WG, Myung IS, Jin KS. Compendium of fruit tree diseases with color plates. Suwon: Agric Sci Tech Inst; 1993.
  13. Liu XJ, Guo YL. Studies on some species of the genus Cercosporidium in China. Acta Mycol Sin 1982;1:88-102.
  14. Shin HD, Braun U. Notes on Korean Cercosporae and allied genera (II). Mycotaxon 1996;58:157-63.
  15. Crous PW, Braun U, Hunter GC, Wingfield MJ, Verkley GJ, Shin HD, Nakashima C, Groenewald JZ. Phylogenetic lineages in Pseudocercospora. Stud Mycol 2013;75:37-114. https://doi.org/10.3114/sim0005
  16. Index fungorum. Species fungorum. Royal Botanic Gardens Kew. [Internet] London: Index fungorum; 2021 [cited Aug 13]. Available from: http://www.indexfungorum.org/names/Names.asp.
  17. Braun U. Miscellaneous notes on phytopathogenic hyphomycetes (II). Mycotaxon 1995;55:223-41.
  18. Deighton FC. New species of Pseudocercospora and Mycovellosiella, and new combinations into Pseudocercospora and Mycovellosiella. Trans Brit Mycol Soc 1987;88:365-91. https://doi.org/10.1016/s0007-1536(87)80011-6
  19. Sivanesan A. The bitunicate Ascomycetes and their anamorph. Vaduz: J Cramer; 1984.