The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Inhibitory Effect of Chitosan and Phosphate Cross-linked Chitosan against Cucumber Mosaic Virus and Pepper Mild Mottle Virus

  • Gangireddygari, Venkata Subba Reddy (Virology Unit, Horticulture and Herbal Crop Environment Division, National Institute of Horticulture and Herbal Science, Rural Development Administration) ;
  • Chung, Bong Nam (Virology Unit, Horticulture and Herbal Crop Environment Division, National Institute of Horticulture and Herbal Science, Rural Development Administration) ;
  • Cho, In-Sook (Virology Unit, Horticulture and Herbal Crop Environment Division, National Institute of Horticulture and Herbal Science, Rural Development Administration) ;
  • Yoon, Ju-Yeon (Virology Unit, Horticulture and Herbal Crop Environment Division, National Institute of Horticulture and Herbal Science, Rural Development Administration)
  • Received : 2021.10.27
  • Accepted : 2021.11.15
  • Published : 2021.12.01
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Abstract

Cucumber mosaic virus (CMV) and Pepper mild mottle virus (PMMoV) causes severe economic loss in crop productivity of both agriculture and horticulture crops in Korea. The previous surveys showed that naturally available biopolymer material - chitosan (CS), which is from shrimp cells, reduced CMV accumulation on pepper. To improve the antiviral activity of CS, it was synthesized to form phosphate cross-linked chitosan (PCS) and compared with the original CS. Initially, the activity of CS and PCS (0.01%, 0.05%, and 0.1% concentration) compound against PMMoV infection and replication was tested using a half-leaf assay on Nicotiana glutinosa leaves. The total number of local lesions represented on a leaf of N. glutinosa were counted and analyzed with phosphate buffer treated leaves as a negative control. The leaves treated with a 0.1% concentration of CS or PCS compounds exhibited an inhibition effect by 40-75% compared with the control leaves. The same treatment significantly reduced about 40% CMV accumulation measured by double antibody sandwich enzyme-linked immunosorbent assay and increased the relative expression levels of the NPR1, PR-1, cysteine protease inhibitor gene, LOX, PAL, SRC2, CRF3 and ERF4 genes analyzed by quantitative reverse transcriptase-polymerase chain reaction, in chili pepper plants.

Keywords

Acknowledgement

This work was supported by a grant from the Basic Research Program (PJ01431803) of the National Institute of Horticultural and Herbal Science, Rural Development Administration, Republic of Korea. The authors are thankful to Dr. M.N. Prabhakar, Dept. of Mechanical Engineering, Changwon National University, Changwon, South Korea, for providing the CS and PCS materials.

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