The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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The Water-Soluble Chitosan Derivative, N-Methylene Phosphonic Chitosan, Is an Effective Fungicide against the Phytopathogen Fusarium eumartii

  • Mesas, Florencia Anabel (Instituto de Investigaciones Biologicas, UE CONICET-UNMDP, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata) ;
  • Terrile, Maria Cecilia (Instituto de Investigaciones Biologicas, UE CONICET-UNMDP, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata) ;
  • Silveyra, Maria Ximena (Instituto de Investigaciones Biologicas, UE CONICET-UNMDP, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata) ;
  • Zuniga, Adriana (INQUISUR, Departamento de Quimica, Universidad Nacional del Sur-CONICET) ;
  • Rodriguez, Maria Susana (INQUISUR, Departamento de Quimica, Universidad Nacional del Sur-CONICET) ;
  • Casalongue, Claudia Anahi (Instituto de Investigaciones Biologicas, UE CONICET-UNMDP, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata) ;
  • Mendieta, Julieta Renee (Instituto de Investigaciones Biologicas, UE CONICET-UNMDP, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata)
  • 투고 : 2021.06.06
  • 심사 : 2021.09.18
  • 발행 : 2021.12.01
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초록

Chitosan has been considered an environmental-friendly polymer. However, its use in agriculture has not been extended yet due to its relatively low solubility in water. N-Methylene phosphonic chitosan (NMPC) is a water-soluble derivative prepared by adding a phosphonic group to chitosan. This study demonstrates that NMPC has a fungicidal effect on the phytopathogenic fungus Fusarium solani f. sp. eumartii (F. eumartii) judged by the inhibition of F. eumartti mycelial growth and spore germination. NMPC affected fungal membrane permeability, reactive oxygen species production, and cell death. Also, this chitosan-derivative exerted antifungal effects against two other phytopathogens, Botrytis cinerea, and Phytophthora infestans. NMPC did not affect tomato cell viability at the same doses applied to these phytopathogens to exert fungicide action. In addition to water solubility, the selective biological cytotoxicity of NMPC adds value in its application as an antimicrobial agent in agriculture.

키워드

과제정보

This research was supported by the Agencia Nacional de Promocion Cientifica y Tecnologica (PICT RAICES 0959), Universidad Nacional de Mar del Plata (EXA 928/19), Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET) and Comision de Investigaciones Cientificas (CIC, 1480/18). FAM is a PhD fellow from CONICET. CAC, MCT and MXS are members of the research staff from CONICET. JRM is researcher from CIC. We thank Dr. Candela Lobato and Dr. Ana Laxalt for providing us fungal strains and tomato cells, respectively. We also thank Dr. Diego Fiol and Dr. Ruben D. Conde for his critical reading of our manuscript. This study was funded by Agencia Nacional de Promocion Cientifica y Tecnologica (PICT RAICES 0959), Universidad Nacional de Mar del Plata (EXA 928/19), CONICET and Comision de Investigaciones Cientificas de la Provincia de Buenos Aires (CIC, 1480/18).

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