The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Bacterial Community and Diversity from the Watermelon Cultivated Soils through Next Generation Sequencing Approach

  • Adhikari, Mahesh (Division of Biological Resource Sciences, Interdisciplinary Program in Smart Agriculture, Kangwon National University) ;
  • Kim, Sang Woo (Division of Biological Resource Sciences, Interdisciplinary Program in Smart Agriculture, Kangwon National University) ;
  • Kim, Hyun Seung (Division of Biological Resource Sciences, Interdisciplinary Program in Smart Agriculture, Kangwon National University) ;
  • Kim, Ki Young (Division of Biological Resource Sciences, Interdisciplinary Program in Smart Agriculture, Kangwon National University) ;
  • Park, Hyo Bin (Division of Biological Resource Sciences, Interdisciplinary Program in Smart Agriculture, Kangwon National University) ;
  • Kim, Ki Jung (Division of Biological Resource Sciences, Interdisciplinary Program in Smart Agriculture, Kangwon National University) ;
  • Lee, Youn Su (Division of Biological Resource Sciences, Interdisciplinary Program in Smart Agriculture, Kangwon National University)
  • 투고 : 2021.07.04
  • 심사 : 2021.09.17
  • 발행 : 2021.12.01
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초록

Knowledge and better understanding of functions of the microbial community are pivotal for crop management. This study was conducted to study bacterial structures including Acidovorax species community structures and diversity from the watermelon cultivated soils in different regions of South Korea. In this study, soil samples were collected from watermelon cultivation areas from various places of South Korea and microbiome analysis was performed to analyze bacterial communities including Acidovorax species community. Next generation sequencing (NGS) was performed by extracting genomic DNA from 92 soil samples from 8 different provinces using a fast genomic DNA extraction kit. NGS data analysis results revealed that, total, 39,367 operational taxonomic unit (OTU), were obtained. NGS data results revealed that, most dominant phylum in all the soil samples was Proteobacteria (37.3%). In addition, most abundant genus was Acidobacterium (1.8%) in all the samples. In order to analyze species diversity among the collected soil samples, OTUs, community diversity, and Shannon index were measured. Shannon (9.297) and inverse Simpson (0.996) were found to have the highest diversity scores in the greenhouse soil sample of Gyeonggi-do province (GG4). Results from NGS sequencing suggest that, most of the soil samples consists of similar trend of bacterial community and diversity. Environmental factors play a key role in shaping the bacterial community and diversity. In order to address this statement, further correlation analysis between soil physical and chemical parameters with dominant bacterial community will be carried out to observe their interactions.

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과제정보

This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, and Forestry (IPET) through Project No. 120088-05-1-SB010 funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA). This research has been worked partially with the support of a research grant of Kangwon National University in 2019 and 2020.

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