Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Anti-cancer effects of Salicornia herbacea extract in OVCAR-3 cells by induction of apoptosis pathway

함초 추출물의 세포사멸 유도에 의한 난소암세포에서 항암 효과

  • La, Yu Ri (Department of Smart Foods and Drugs, Inje University) ;
  • Lee, You Rim (Department of Smart Foods and Drugs, Inje University) ;
  • Lee, Dong Seok (Department of Smart Foods and Drugs, Inje University) ;
  • Kim, Soo Hwan (Department of Biomedical Laboratory Science, Gimcheon University) ;
  • Lee, Hyeong Seon (Department of Biomedical Laboratory Science, Jungwon University)
  • 라유리 (인제대학교 일반대학원 식의약생명공학과) ;
  • 이유림 (인제대학교 일반대학원 식의약생명공학과) ;
  • 이동석 (인제대학교 일반대학원 식의약생명공학과) ;
  • 김수환 (김천대학교 헬스케어대학 임상병리학과) ;
  • 이형선 (중원대학교 의료보건대학 임상병리학과)
  • Received : 2020.10.26
  • Accepted : 2020.12.29
  • Published : 2021.02.28
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Abstract

This study investigated the anti-cancer effects of Salicornia herbacea L. fractions in human ovarian cancer cells (OVCAR-3). S. herbacea powder was extracted with 95% EtOH and sequentially fractionated with hexane, dichloromethane (DCM), ethyl acetate, butanol, and H2O. Further, the growth inhibitory effects of the six fractions were determined using the MTS assay. The DCM fraction dramatically decreased cell viability. Similarly, the cell cycle was arrested at the subG1 phase in DCM-treated cells. To confirm apoptosis, the cells were stained with annexin V/FITC-PI solution. Total, early, and late apoptotic cells were significantly increased in the DCM fraction. The mRNA expression of Bcl-2 was reduced, whereas the pro-apoptotic factors Bax and Bak were increased in DCM fraction-treated cells. These results indicated that the DCM fraction in S. herbacea exhibited strong apoptotic effects through the p53-dependent signaling pathway.

본 연구에서는 천연유래 물질로부터 apoptosis를 유도하여 항암 활성이 있는지에 관한 실험으로 사람 난소암 세포주인 OVCAR-3 세포에 함초의 유기용매별 추출물을 처리하여 결과를 확인하였다. MTS 측정으로 세포 생존율을 확인한 결과 DCM 분획물에서 농도별로 유의적인 세포수의 감소를 보였으며, annexin V/FITC-PI 염색에 의해 apoptosis 유도로 세포가 사멸함을 확인하였다. DCM 분획물 처리는 세포주기에서 Sub-G1기의 증가로 세포증식이 억제됨을 보여준다. 세포의 내인성 경로에 관여하는 Bcl-2 family에 속하는 Bax, Bak, Bcl-2, Bcl-xL의 상호작용을 mRNA 수준에서 확인한 결과, DCM 분획물처리에서 Bax, Bak가 증가하고, Bcl-2의 감소를 동반하여 세포사멸의 신호전달 경로가 진행됨을 확인할 수 있었다. 본 연구 결과를 바탕으로 함초를 이용하여 여성의 난소암에 예방적 기능성 식품을 개발할 수 있을 것으로 판단되며, 함초의 DCM 분획물에 대해 깊이 있는 심층적 연구가 요구된다.

Keywords

References

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