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Physiologically active components and vasorelaxation effect of Vitis labruscana B. and Vitis coignetiae grapevine leaves at growth stages

켐벨얼리(Vitis labruscana B.)와 머루(Vitis coignetiae) 포도잎의 생육단계별 생리 활성 성분 및 혈관 이완능

  • Yu, Jin-Ju (Hanpoong Pharm & Foods Co.) ;
  • Kim, Hye-Yoom (Hanbang Cardio-Renal Syndrome Research Center, Wonkwang University)
  • 유진주 (한풍제약) ;
  • 김혜윰 (원광대학교 한방심신증후군연구센터)
  • Received : 2020.11.30
  • Accepted : 2021.01.26
  • Published : 2021.02.28

Abstract

Prior studies of physiologically active substances in grapes have focused only on the fruit, pericarp, and branches, as well as the pulp and seeds. The present study assessed the changes in quercetin-3-O-glucuronide (Q3OG) and flavonol glycoside content using grape leaves from Vitis labruscana B. and V. coignetiae at different growth stages and provided basic data for quality control. Content analysis showed that both varieties differed in Q3OG and flavonol glycoside content by growth stage, and the components were found to be high in the order of fruit, maturation, and coloration. Also, Vitis labruscana B. has a better vascular relaxation effect than Vitis coignetiae. These results suggest that in the use of grape leaves as a functional raw material, Q3OG and flavonol glycosides can be used as indicator components. In addition, if raw materials for each growth stage are mixed in a particular ratio, it will be a way to manage the specific efficacy and content of indicator components.

본 연구의 목적은 켐벨얼리 품종과 머루 품종의 포도 잎을 생육단계별로 채취하여 Q3OG 및 플라보놀 배당체(quercetin, kaempferol, isorhamnetin)의 함량 변화와 혈관 이완 효과를 평가하여 기능성 소재로 활용하기 위한 품질관리 기초 자료를 제공하는 것이다. 포도의 생육단계는 전엽기, 개화기, 결실기, 착색기 및 성숙기로 구분되는데, 산업적으로 포도 잎 원료 확보가 쉬운 결실기, 착색기 및 성숙기 포도 잎을 대상으로 분석했다. 시험 결과 두 품종 모두 생육단계별로 Q3OG 및 플라보놀 배당체 함량의 차이가 있었고, 결실기>성숙기>착색기 순으로 성분들의 함량이 높은 것으로 확인되었다. 생육단계별 혈관 이완 효과는 두 품종 모두 혈관 이완 효과를 보였으며 켐벨얼리 포도 잎이 머루 포도 잎보다 더 좋은 것으로 나타났다. 이는 포도잎 추출물의 기능성 소재 활용 가능성을 보여주었다. 물론, 안타깝게도 Q3OG 및 플라보놀 배당체 성분이 혈관 이완 효과를 대표하는 효능성분은 아닌 것으로 판단되지만, 착색기 포도 잎에 비하여 결실기와 성숙기에 함량이 높은 Q3OG 및 플라보놀 배당체가 지표성분으로 써의 가치는 있다고 판단된다. 따라서 포도 잎을 기능성 원료로 활용 시 Q3OG 및 플라보놀 배당체를 지표성분으로 활용하고, 각 생육단계별 포도 잎 원료를 일정 비율로 혼합하여 사용하면 일정한 효능과 지표성분 함량을 관리하는 방법이 되리라 생각한다.

Keywords

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