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Effect of Antimicrobiotic of Opuntia ficus-indica on Surface Disinfection

제주도 손바닥선인장 추출물의 표면소독제로써의 항균효과

  • Kwon, Young-ran (College of Nursing, Kyungpook National University) ;
  • Kim, Da-Song (Biochemical & Polymer Engineering, Chosun National University) ;
  • Shin, Hyun-Jae (Biochemical & Polymer Engineering, Chosun National University)
  • 권영란 (경북대학교 간호대학) ;
  • 김다송 (조선대학교 생명화학고분공학과) ;
  • 신현재 (조선대학교 생명화학고분공학과)
  • Received : 2020.04.15
  • Accepted : 2021.02.24
  • Published : 2021.02.28

Abstract

Purpose: To verify the anti-microbiotic activity and to develop eco-friendly surface disinfectants using the Opuntia ficus-indica extract. Methods: The stem and fruits of the Opuntia cultivated in Jeju Island were extracted with hot water, 70% EtOH and 100% MeOH, respectively. To examine the antimicrobial activity of the extracts, gram positive bacteria (Bacillus subtilis, Staphylococcus aureus), gram negative bacteria (Escherichia coli, Salmonella typhimurium), yeast (Candida albicans), mold (Aspergillus flavus) and antibiotic resistant bacteria (E. coli, S. aureus) were measured to form a clear zone. Results: The antibacterial activity of the fruit extract was higher than that of the stem extract, and the antibacterial activity of the 100% MeOH (FM) extract and the 70% EtOH (FE) extract of the palm cactus fruit was suitable for the surface disinfectant overall, and clear-zone formed even at relatively low concentrations (250 mg/mL). The FM and FE have antibacterial activity against various bacteria, therefore they can be used as disinfectants. Conclusion: The antimicrobial activity of Opuntia ficus-indica extract from Jeju Island by stem and fruit was determined at relatively low concentrations (250 mg/mL). The Opuntia ficus-indica extract will be useful for surface disinfectants dissolving the patients' bath, oral gargling, and powdered powder in water or ethanol in appropriate concentrations.

Keywords

References

  1. Kim YH. Pathogen Micrology. 4th ed. Seoul: Hyeonmunsa; 2008. p.106-110.
  2. Park ES, Jang SY, Kim OS, Yoon SW, Jeong JS, Kim KM, et al. Descriptive study on the current usage of disinfectants in korea. Korean Jounal of Nosocomial Infection Control and Prevention. 2006;11(1):42-49. https://doi.org/10.14192/kjicp.2006.11.1.42.
  3. Rutala WA, Weber DJ. Modern advances in disinfection, sterilization, and medical waste management in wenzel RP, editor. Prevention and Control of Nosocomial Infections. 4th ed. Philadelphia: Lippincott Williams & Wilkins; 2003. p.542-574.
  4. Jeong SY, Choi JH, Kim EK, Kim SM, Son HJ, Cho NH, et al. Actual disinfection and sterilization control in korean healthcare facilities. Journal Korean Academy Fundamentals of Nursing. 2014;21(4):392-402. https://doi.org/10.7739/jkafn.2014.21.4.392.
  5. Moon C, Kim KM. In vitro inhibitory effect of alcohol hand rubs against staphylococcus aureus. Journal of Korean Biological Nursing Science. 2010;12(2):73-80.
  6. Yoon YK, Sim HS, Kim JY, Park DW, Sohn JW, Roh KH, et al. Epidemiology and control of an outbreak of vancomycin-resistant enterococci in the intensive care units. Yonsei Medical Journal. 2009;50(5):637-643. https://doi.org/10.3349/ymj.2009.50.5.637
  7. Rutala WA, Weber DJ, The healthcare infection control practices advisory committee (HICPAC). Guideline for disinfection and sterilization in healthcare facilities (2008) [Internet]. Atlanta: Center for Diseases Control and Prevention; 2019 May [cited 2019 May 24]. Available from: https://www.cdc.gov/infectioncontrol/guidelines/disinfection/
  8. Rutala WA, Weber DJ. The benefits of surface disinfection. American Journal of Infect Control. 2004;32(4):226-231. https://doi.org/10.1016/j.ajic.2004.04.197
  9. MaryAnn TS, Deborah LC. Infection control in the dental office. 12. Chapter Surface Disinfection [Internet]. Switzerland: Springer; 2020 [Cited 2019 Nov 18]. Available from:https://link.springer.com/chapter/10.1007/978-3-030-30085-2_12
  10. Song X, Vossebein L, Zille A. Efficacy of disinfectant-impregnated wipes used for surface disinfection in hospitals: a review. Antimicrobial Resistance and Infection Control. 2019;8:139. https://doi.org/10.1186/s13756-019-0595-2
  11. Centers for Diseases for Control and Prevention. Guidelines for disinfection and sterilization in healthcare facilities (2008) [Internet]. Atlanta: National Center for Emerging and Zoonotic Infectious Diseases, Division of Healthcare Quality Promotion; 2008 [cited 2016 Sep 18] Available from: https://www.cdc.gov/infectioncontrol/guidelines/disinfection/disinfection-methods/chemical.htm
  12. Lee CK, Lee YC, Moon YN, Park HJ, Han YN, Choi JW. Screening on biological activities of the extracts from fruit and stem of prickly pear (Opuntia ficusindica var. saboten). Korean Journal of Pharmacognosy. 2001;32(4):330-337.
  13. Jung EJ, Hong SJ, Choi J, Jeong SS, Oh HN, Lee HJ, et al. In vitro growth inhibition of streptococcus mutans by extract of prickly pear (Opuntia ficus-indica var. saboten makino). Journal of Korean Academy of Oral Health. 2010;34(1): 28-35.
  14. Lee YC, Hwang KH, Han DH, Kim SD. Compositions of opuntia ficus-indica. Korean Journal of Food Science and Technology. 1997;29(5):847-853.
  15. Lee KS, Kim MK, Lee KY. Antimicrobial effect of the extracts of cactus chounnyouncho (Opuntia humifusa) against food borne pathogen. Journal Korean Society Food Science Nutrition. 2004;33(8):1268-1272. https//doi.org/10.3746/jkfn.2004.33.8.1268
  16. Chung HJ. Antioxidative and antimicrobial activities of Opuntia ficus indica var. saboten. Korean Journal of Food and Cookery Science. 2000;16(2):160-166.
  17. Kim HN, Kwon DH, Kim HY, Chun HK. Antimicrobial activities of opuntia ficus-indica var. saboten makino methanol extract. Journal of Life Science.2005; 5(2):279-286, https://doi.org/10.5352/JLS.2005.15.2.279
  18. Gnanakalai K, Gopal R. In vitro antibacterial activities of Opuntia ficus indica stem and fruit extracts using disc diffusion method. International Journal of Current Pharmaceutical Research. 2016;8(2):68-69. https://doi.org/10.22159/ijcpr.2016v8i4.15282
  19. Kim HN, Cho DW, Yoon UC, Chun HK. Identification of anti-microbial material originated from Opuntia ficus indica var saboten Makino. Journal of Life Science. 2007;17(7):915-922. https://doi.org/10.5352/JLS.2007.17.7.915
  20. Lee KS, Lee KY. Biological activity of phenol compound from a cactus cheonnyuncho (opuntia humifusa) in Korea. Journal of Korea Society Food Science Nutrition. 2010;39(8) :1132-1136. https//doi.org/10.3746/jkfn.2010.39.8.1132.
  21. Kwon JH, Kim TY, Kim JK, Kim JY. Characteristics of Opuntia monacantha haw. for the functional raw material production. Applied Chemistry for Engineering. 2017;28(2):252-256. https//doi.org/10.14478/ace.2017.1013
  22. Chung BM, Shin MO. Physiological activities of Opuntia humifusa petal. Korean Journal Food and Cookery Science. 2011;27(5):523-530. https://doi.org/10.9724/kfcs.2011.27.5.523
  23. Cho JJ, Kim HS, Kim CH, Cho SJ. Interaction with polypenols and biotics review. Journal of Life Science. 2017;27(4):476-481. https://doi.org/10.5352/JLS.2017.27.4476
  24. Cho IK, Seo KS, Kim YD. Antimicrobial activities, antioxidant effects, and total polyphenol contents of extracts of prickly pear, opuntia ficus indica. Korean Journal of Food Preservation. 2009;16(6):953-958.
  25. Cushnie TPT, Lamb AJ. Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents. 2005;26(5):343-356. https://doi.org/10.1016/j.ijantimicag.2005.09.002.
  26. Seoul National University Natural Products Research Institute. Korean Natural Products Science Research. 1st ed. Seoul: Seoul National University publishing department; 1996. p.23-72.
  27. Bargougui A, Tag HM, Bouaziz M, Triki S. Antimicrobial, antioxidant, total phenols and flavonoids content of four cactus (Opuntia ficus-indica) cultivars. Biomedical and Pharmacology Journal. 2019;12(3):1353-1368. https://doi.org/10.13005/bpj/1764.
  28. Choi JY. Results of research on the development of disinfection and sterilization guidelines for the current-medical period of domestic infection control. Korean Journal of Gastrointestinal Endoscopy Nurses and Associates. 2013;21(2):119-229
  29. Kwon MC, Lee HY, Choi YB, Ko JR, Han JG, Jung HS, et al. Enhancement of immune activities of opuntia ficus-indica L. Miller by ultrasonification extraction process. Korean Journal Medicinal Crop Science. 2008;16(1):1-8.
  30. Whalen Karen. Lippincott's illustrated review: pharmacology. 6th ed. Lim DY translators. Philadelphia, PA: Lippincott Williams & Wilkins; 2014. p.484-485