Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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A Study on the Antioxidative Effect of Orostachys Japonicus A. Berger Ethyl Acetate Fraction

와송 에틸아세테이트 분획물의 항산화 효능에 관한 연구

  • Im, Eun Kyung (Mokwon University, College of Sciences & Technology, Division of Chemical & Cosmetics) ;
  • Yang, Jae Chan (Mokwon University, College of Sciences & Technology, Division of Chemical & Cosmetics)
  • 임은경 (목원대학교 테크노과학대학 화학.화장품학부) ;
  • 양재찬 (목원대학교 테크노과학대학 화학.화장품학부)
  • Received : 2020.01.25
  • Accepted : 2021.02.18
  • Published : 2021.02.28
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Abstract

In this study Orostachys japonica A. Berger used is a medicinal herb that has long been used as a folk remedy for cancer treatment. In this study, the antioxidant efficacy of the ethyl acetate fraction of Orostachys japonica A. Berger was confirmed. The results of the Orostachys japonica A. Berge ethyl acetate fraction of antioxidant activity assays showed Antioxidant effect of Orostachys japonica A. Berger EtOAc fraction extract at 0.10 mg/mL was showed a DPPH radical scavenging rate of 78.54% and ABTS+ radical scavenging rate of 73.48%. Also, the toxicity result of Orostachys japonica A. Berger EtOAc fraction extracts using alternative experimental animal model zebrafish, confirmed a 100% the survival of the zebrafish embryo was shown that there was no coagulation and no hatching delay at all concentrations. also ROS generation induced by UV-B irradiation was confirmed that the fluorescence intensity decreased as a whole in all larvae treated with Orostachys japonica A. Berger EtOAc fraction extracts. In particular, it was confirmed that ROS generation was effectively suppressed by showing a 35.7% reduction rate compared to the positive control at a concentration of 3 ㎍/mL. These results were confirmed that Orostachys japonica A. Berger EtOAc fraction extracts has the possibility of application in the cosmetics field as a natural antioxidant.

본 연구에서 사용된 와송(Orostachys japonica A. Berger)은 암 치료를 위한 민간요법으로 오랫동안 사용되어 온 약초이다. 본 연구에서는 와송(Orostachys japonica A. Berger) 에틸아세테이트 분획물의 항산화 효능 활성에 대하여 실험한 결과를 보고한다. 와송(Orostachys japonica A. Berger) 에틸아세테이트 분획물의 항산화 효능은 0.10 mg/mL 농도에서 78.54%의 DPPH 라디칼 소거활성을 보였고, 73.48%의 ABTS+ 라디칼 소거활성을 나타내어 항산화 효과가 있는 것을 확인하였다. 또한 대체실험동물 모델인 제브라피쉬를 이용한 독성실험 결과에서는 모든 농도에서 100%의 배아 생존율을 보이고 응고 또는 부화지연을 관찰할 수 없어 독성이 없는 것으로 나타났으며, UV-B조사에 의해 유도된 ROS생성은 와송 에틸아세테이트 분획물을 처리한 모든 larvae에서 전체적으로 형광강도가 감소하였다. 특히 3 ㎍/mL 농도에서 양성대조군 대비 35.7%의 감소율을 보여 효과적으로 ROS 생성을 억제하였음을 확인하였다. 위와 같은 결과로 미루어보아, 와송 에틸아세테이트 분획물은 천연 항산화제로서 화장품 분야에서 응용 가능성이 있을 것으로 기대한다.

Keywords

References

  1. M. Wlaschek, I. Tantcheva-Poor, L. Naderi, et al. "Solar UV irradiation and dermal photoaging", Journal Photochemistry and Photobiology B: Biology, Vol. 63, pp. 41-51, (2001). https://doi.org/10.1016/S1011-1344(01)00201-9
  2. S. M. Jeon, "Antioxidavtive componets of Suaeda asparagoides and it's application to cosmetics", Seoul National University of Technology, pp. 14, (2008).
  3. J. R. Jang, S. Y. Hwang, S. Y. Lim, "Effects of Extracts from Dried Yam on Antioxidant and Growth of Human Cancer Cell Lines", Journal of Life Science, Vol. 20, No. 9, pp. 1365-1372, (2010). https://doi.org/10.5352/JLS.2010.20.9.1365
  4. H. G. Yang, H. J. Kim, H. S. Kim, S. N. Park, "Original article : Ethosome Formulation for Enhanced Transdermal Delivery of Artemisia princeps Pampanini Extracts", Applied Chemistry for Engineering, Vol. 24, No. 2, pp. 190-195, (2013).
  5. J. H. Shim, S. Y. Park, "Anti-aging Effects of Diospyros kaki Thunb. Extracts in UVA-irradiated Epidermal Keratinocytes", Asian Journal of Beauty and Cosmetology, Vol. 15, No. 2, pp. 170-178, (2017). https://doi.org/10.20402/ajbc.2016.0121
  6. M. S. Lim, D. S. Lee, S. S. Kwon, S. N. Park, "Research Papers : Stability Test for the Cream Containing Chamaecyparis Obtusa Leaf Extract", Journal of the Korean Oil Chemists Society, Vol. 29, No. 2, pp. 205-213, (2012).
  7. B. G. Lee, "Skin Care Effects of Green Tea", Journal of the society of cosmetic scientists of korea, Vol. 31, No. 4, pp. 311-321, (2005).
  8. S. N. Park, S. K. Han," Extraction, Component Analysis and Antibacterail Activity of Panax ginseng Absolute Essential Oil", Journal of the society of cosmetic scientists of korea, Vol. 34, No. 1, pp. 67-73, (2008).
  9. D. S. Kim, "Natural Healing Revolution: Immunity-enhancing Cancer Treatment Method of Rural Oriental Doctor Kim Dong-Seok", Sangsang Publishing, (2011).
  10. S. H. Jeon, D. O. Hong, C. W. Lee, H. Y. Kim, S. C. Shin J. H. Kang, "Growth and flowering of Orostachys japonicus A. Berger affected by transplanted seedling size", Korean Journal of Medicinal Crop Science, Vol. 14, No. 3, pp. 153-157, (2006).
  11. J. Kwon, S. H. Kwang," Effect of Orostachys japonicus A. Berger on the lmmune system". Korean Journal of Medicinal Crop Science, Vol. 12, No. 4, pp. 315-320, (2004).
  12. S. Y. Yoon, S. Y. Lee, K. B. W. R. Kim, E. J. Song, S. J. Kim, S. J. Lee, C. J. Lee, D. H. Ahn, "Antimicrobial Activity of the Solvent Extract from Different Parts of Orostachys japonicus", Journal of the Korean Society of Food Science and Nutrition, Vol. 38, No. 1, pp. 14-18, (2009). https://doi.org/10.3746/JKFN.2009.38.1.014
  13. S. Y. Choi, "Inhibitory Effects of Orostachys japonicus Extracts on the Formation of N-Nitrosodimethylamine", Dissertation, University of Gyeongsang, (2006).
  14. P. Molyneux, "The use of the Stable Free Radical Diphenylpicrylhydrazyl(DPPH) for Estimating Antioxidant Activity", Journal of Science and Technology, Vol. 26, No. 2, pp. 211-219, (2004).
  15. H. H. Lee, Y. S. Moon, "Assessment of the Important Factors Influencing Consistent and Accurate ABTS Assay", Journal of the Korean Society of Food Science and Nutrtion, Vol. 48, No. 3, pp. 390-394, (2019). https://doi.org/10.3746/jkfn.2019.48.3.390
  16. S. I. Kim, D. G. Kim, J. B. Park, T. K. Lee, "DPPH Radical Scavenging, and Tyrosinase Inhibitory Activities of Ecklonia cava Extracted with the Ultrasonic Wave Method", Journal of Life Science, Vol .23, No. 7, pp. 913-918, (2013). https://doi.org/10.5352/JLS.2013.23.7.913
  17. B. G. Lee, J. H. Kim, S. G. Ham, C. E. Lee, "Study on Biological Activities of Extracts for Cosmeceutical Development from Lagerstroemia indica L. Branch", Korean Journal of Plant Resources, Vol. 27, No. 1, pp. 29-34, (2014). https://doi.org/10.7732/kjpr.2014.27.1.029
  18. S. M. Kim, J. H. Park, H. O. Boo, S. G. Song, H. Y. Park, "In vitro Comparision of Biological Activities of Solvent Fraction Extracts from Orostachys japonicus", Korean Journal of Plant Resources, Vol. 30, No. 2. pp. 133-143, (2017). https://doi.org/10.7732/kjpr.2017.30.2.133
  19. S. B. Park, D. S. Lee, J. Y. Kang, J. M. Kim, S. K. Park, J. E. Kang, B. S. Kwon, S. H. Park, C. J. Lee, U. Lee, H. J. Heo, "Protective effect on neuronal cells of Orostachys japonicus A. Berger extract against reactive oxygen species-induced neuronal cytotoxicity and active compounds", Korean Society of Food Science and Technology, Vol. 49, No. 5, pp. 524-531, (2017).
  20. Ho-Hyeon Lee, Yong-Sun Moon, "Assessment of the Important Factors Influencing Consistent and Accurate ABTS Assay", Journal of the Korean Society of Food Science and Nutrition, Vol. 48, No. 3, pp. 390-393, (2019). https://doi.org/10.3746/jkfn.2019.48.3.390
  21. Y. M. Lee, J. H. Bae, H. Y. Jung, J. H. Kim, D. S. Park, "Antioxidant Activity in Water and Methanol Extracts from Korean Edible Wild Plants", Journal of the Korean Society of Food Science and Nutrition, Vol. 40, No. 1, pp. 29-36, (2011). https://doi.org/10.3746/JKFN.2011.40.1.029
  22. P. Nemtsas, E. Wettwer, T. Christ, G. Weidinger, U. Ravens, "Adult zebrafish heart as a model for human heart? An electrophysiological study", Journal of Molecular and Cellular Cardiology, Vol. 48, pp. 161-171, (2010). https://doi.org/10.1016/j.yjmcc.2009.08.034
  23. M. J. Kim, E. J. Park, "Feature Analysis of Different In Vitro Antioxidant Capacity Assays and Their Application to Fruit and Vegetable Samples", Journal of the Korean Society of Food Science and Nutrition, Vol. 40, No. 7, pp. 1053-1062, (2011). https://doi.org/10.3746/JKFN.2011.40.7.1053