Korean Journal of Clinical Laboratory Science (대한임상검사과학회지)

Korean Society for Clinical Laboratory Science (대한임상검사과학회)
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Effects of Aucklandia lappa Decne. Extract on Hair Growth in Depilated CL57BL/6 Mice

CL57BL/6 마우스의 발모에 미치는 목향 추출물의 영향

  • Kim, Joung-Hee (Department of Biomedical Laboratory Science, Daekyeung University) ;
  • Lee, Syng-Ook (Department of Food Science and Technology, Keimyung University) ;
  • Beik, Gyoung Yeun (Okchundang Research institute) ;
  • Kim, Keuk-Jun (Department of Biomedical Laboratory Science, Daekyeung University)
  • Received : 2021.02.04
  • Accepted : 2021.02.22
  • Published : 2021.03.31
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Abstract

Aucklandia lappa Decne., a traditional herbal medicine, has been used to treat various diseases, including asthma, coughs, rheumatism, and diarrhea. In the present study, the effects of the oral administration of Aucklandia lappa Decne. extract on hair growth was investigated in hair-removed mice. A 70% ethanol extract of Aucklandia lappa Decne. (ALD) was prepared, and the extraction yield and total polyphenol content of ALD were measured as 27.30±0.01 and 28.39 mg gallic acid equivalent (GAE)/g, respectively. The oral administration of ALD to hair-removed CL57BL/6 mice for six weeks had no significant effects on food intake and body weight changes. Biochemical and histological examinations also showed that the oral administration of ALD for six weeks had no significant effect on the liver and kidney functions. On the other hand, hair growth was significantly higher in the ALD group than the control group and the Pancidil group (positive control). In addition, the number of hair follicles and the degree of collagen production in the dermis were significantly higher in the ALD group than in the control and pancidil groups. These results suggest that ALD is a potential source of nutricosmetics with hair growth-promoting effects.

본 연구에서는 제모된 마우스 모델을 대상으로 목향 추출물의 경구 투여가 발모에 미치는 영향에 대해 조사하였다. 목향 70% prethanol 추출물(ALD)을 제조하여 추출수율과 추출물에 함유된 총 폴리페놀 함량을 측정한 결과 각각 27.30±0.01과 28.39 mg gallic acid equivalent (GAE)/g extract로 측정되었다. ALD를 제모된 CL57BL/6 마우스에 6주간 경구 투여하며 관찰한 결과, 식이량과 체중 변화에 유의적인 변화가 관찰되지 않았다. 혈액 생화학적 검사 및 조직학적 검사를 통해 ALD의 6주간 반복 투여가 주요 장기인 간과 신장의 기능에 미치는 영향을 살펴본 결과, 대조군에 비해 간기능과 콩팥기능 모두 유의적인 변화는 관찰되지 않았다. 이와 더불어 ALD 투여에 따른 모발 성장 정도를 육안적으로 관찰한 결과, ALD 투여군에서 대조군 및 판시딜 투여군(양성 대조군)보다 현저하게 발모가 증가함을 확인하였다. 또한 모낭수와 진피의 콜라겐 생성 정도를 비교한 결과, 대조군 및 판시딜 투여군 대비 ALD 투여군에서 두 항목 모두 유의적으로 증가함을 알 수 있었다. 본 연구로부터 ALD의 경구 투여는 제모된 마우스 모델에서 모발 성장을 촉진하며 진피내 콜라겐 합성 증가와 모낭수 증가를 유도한다는 결론을 도출할 수 있었으며, 이는 ALD를 함유하는 경구용 발모 촉진제 개발을 위한 기초자료로써 그 가치가 있을 것으로 기대된다.

Keywords

References

  1. Lee SH, Lee JR. Association of diffuse hair loss of adult male on stress, self-confidence, and depression. J Kor Soc Cosm. 2010;16:1171-1179.
  2. Patel M, Harrison S, Sinclair R. Drugs and hair loss. Dermatologic Clinics. 2013;31:67-73. https://doi.org/10.1016/j.det.2012.08.002
  3. Oh JW, Kloepper J, Langan EA, Kim YS, Yeo JY, Kim MJ, et al. A guide to studying human hair follicle cycling in vivo. J Invest Dermatol. 2016;136:34-44. https://doi.org/10.1038/JID.
  4. Buhl AE, Waldon DJ, Miller BF, Brunden MN. Differences in activity of minoxidil and cyclosporin A on hair growth in nude and normal mice. Lab Invest. 1990;62:104-107.
  5. Hattori M, Oqawa H. Biochemical analysis of hair growth from the aspects of aging and enzyme activities. J Dermatol. 1983;10:45-54. https://doi.org/10.1111/j.1346-8138.1983.tb01103.x
  6. Inaoka Y, Shakuya A, Fukazawa H, Ishida H, Nukaya H, Tsuji K, et al. Studies on active substances in herbs used for hair treatment. I. Effects of herb extracts on hair growth and isolation of an active substance from Polyporus umbellatus F. Chem Pharm Bull. 1994;42:530-534. https://doi.org/10.1248/cpb.42.530
  7. Kim SH, Kim YH, Han SS, Roh JK. Teratogenicity study of scutellariae radix in rats. Reprod Toxicol. 1993;7:73-79. https://doi.org/10.1016/0890-6238(93)90012-v
  8. Kessels AG, Cardynaals RL, Borge RL, Go MJ, Lambers JC, Knottnerus JA, et al. The effectiveness of the hair-restorer "Dabao" in males with alopecia androgenetica. A clinical experiment. J Clin Epidemiol. 1991;44:439-447. https://doi.org/10.1016/0895-4356(91)90083-l
  9. Ishimatsu TY, Soma T, Kishimoto J. Identification of novel hair-growth inducers by means of connectivity mapping. FASEB J. 2010;24:1489-1496. https://doi.org/10.1096/fj.09-145292
  10. Wosicka H, Cal K. Targeting to the hair follicles: current status and potential. J Dermatol Sci. 2010;57:83-89. https://doi.org/10.1016/j.jdermsci.2009.12.005
  11. Ali A, Martin JM. Hair growth in patients alopecia areata totalis after treatment with simvastatin and ezetimibe. J Drugs Dermatol. 2010;9:62-64.
  12. Yoon JI, Al-Reza SM, Kang SC. Hair growth promoting effect of Zizyphus jujuba essential oil. Food Chem Toxicol. 2010;48:1350-1354. https://doi.org/10.1016/j.fct.2010.02.036
  13. Dufton N, Perretti M. Therapeutic anti-inflammatory potential of formyl-peptide receptor agonists. Pharmacol Ther. 2010;127:175-188. https://doi.org/10.1016/j.pharmthera.2010.04.010
  14. Gregoriou S, Papafragkaki D, Kontochristopoulos G, Rallis E, Kalogeromitros D. Rigopoulos D. Cytokines and other mediators in alopecia areata. Mediators of Inflammation. 2010;2010:928030. https://doi.org/10.1155/2010/928030
  15. Kim SY, Kim JH, Kim SK, Oh MJ. Antioxidant activities of selected oriental herb extracts. J Am Oil Chemists' Soc. 1994;71:633-640. https://doi.org/10.1007/BF02540592
  16. Wedge DE, Galindo JC, Macias FA. Fungicidal activity of natural and synthetic sesquiterpene lactone analogs. Phytochemistry. 2020;53:747-757. https://doi.org/10.1016/s0031-9422(00)00008-x
  17. Lee MS, Ryu DG, Kwon KB. Anti-inflammatory effects of Saussurea Lappa extracts in murine macrophages. J Oriental Physiology & Pathology. 2011;25:275-279.
  18. Jeong SJ, Itokawa T, Shibuya M, Kuwano M, Ono M, Higuchi R, et al. Costunolide, a sesquiterpene lactone from Saussurea lappa, inhibits the VEGFR KDR/Flk-1 signaling pathway. Cancer Lett. 2002;187:129-133. https://doi.org/10.1016/s0304-3835(02)00361-0
  19. Zine A, Ribaupierre F. Replacement of mammalian auditory hair cells. Neuro Report. 1998;9:263-268. https://doi.org/10.1097/00001756-199801260-00016
  20. Lin WH, Xing LJ, Shi HX, Zhang J, Jiang L, Cai PT, et al. Fibroblast growth factors stimulate hair growth through β-Catenin and shh expression in C57BL/6 mice. Biomed Res Int. 2015;2015:730139. https://doi.org/10.1155/2015/730139
  21. Braun S, Krampert M, Bodo E, Kumin A, Christiane BB, Paus R, et al. Keratinocyte growth factor protects epedermis and hair follicles from cell death induced by UV irradiation, chemotherapeutic or cytotoxic agents. J Cell Science. 2006;119:4841-4849. https://doi.org/10.1242/jcs.03259
  22. Zhang X, Wang Y, Gao Y, Liu X, Bai T, Li M, et al. Maintenance of high proliferation and multipotent potential of human hair folliclederived mesenchymal stem cells by growth factors. Int J Mol Medicine. 2013;31:913-921. https://doi.org/10.3892/ijmm.2013.1272
  23. Kim MS, Kim NS, Kwon J, Kim HR, Lee DY, Oh MJ, et al. Anti-inflammatory and immune regulatory effects of Aucklandia lappa decne 70% ethanol extract. J Medicinal Crop Science. 2018;26:8-18. https://doi.org/10.7783/KJMCS.2018.26.1.8
  24. Lim JS, Lee SH, Lee SR, Lim HJ, Roh YS, Won EJ, et al. Inhibitory effects of Aucklandia lappa decne. Extract on inflammatory and oxidative responses in LPS-treated macrophages. Molecules. 2020;25:1336. https://doi.org/10.3390/molecules25061336
  25. Park KT, Oh SL, Cho YJ. Hyaluronidase inhibitory activity of extracted phenolic compounds from ultrafine grind Saururus chinensis. J Food Preservation. 2016;23:20-26. https://doi.org/10.11002/kjfp.2016.23.1.20
  26. Plonka PM, Michalczyk D, Popik M, Handjiski B, Slominski A, Paus R. Splenic eumelanin differs from hair eumelanin in C57BL/6 mice. Acta Biochimica Polonica. 2005;52:433-441. https://doi.org/10.18388/abp.2005_3456
  27. Peus D, Pittelkow MR. Growth factors in hair organ development and the hair growth cycle. Dermatologic Clinics. 1996;14:559-572. https://doi.org/10.1016/S0733-8635(05)70384-3
  28. Hue1 JJ, Jo BK, Kang BS, Kim Jh, Nam SY, Yun YW, et al. Effect of Sodium Silicate on hair growth in C57BL/6 mice. Lab Anim Res. 2010;26:55-62. https://doi.org/10.5625/lar.2010.26.1.55
  29. Seaborn CD, Nielsen FH. Silicon deprivation decreases collagen formation in wounds and bone, and ornithine transaminase enzyme activity in liver. Biol Trace Elem Res. 2002;89:251-261. https://doi.org/10.1385/bter:89:3:251