Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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A Study on the Activities of Five Natural Plant Essential Oils on Atopic Dermatitis

자생식물 Essential Oil 5 종의 항 아토피피부염 활성 연구

  • Jeong, Jeong-Hwa (Dongje Medical) ;
  • Nguyen, Thao Kim Nu (Vietnam Type Culture Collection, Institute of Microbiology and Biotechnology, Vietnam National University) ;
  • Choi, Min-Jin (Department of Physiology, College of Korean Medicine, Dongguk University) ;
  • Nguyen, Ly Thi Huong (Department of Physiology, College of Korean Medicine, Dongguk University) ;
  • Shin, Heung-Mook (Department of Physiology, College of Korean Medicine, Dongguk University) ;
  • Lee, Byung-Wook (Department of Medical Classics and History, College of Korean Medicine, Dongguk University) ;
  • Yang, In-Jun (Department of Physiology, College of Korean Medicine, Dongguk University)
  • 정정화 ((주)동제메디칼) ;
  • ;
  • 최민진 (동국대학교 한의학과 생리학교실) ;
  • 응웬리 (동국대학교 한의학과 생리학교실) ;
  • 신흥묵 (동국대학교 한의학과 생리학교실) ;
  • 이병욱 (동국대학교 한의학과 원전의사학교실) ;
  • 양인준 (동국대학교 한의학과 생리학교실)
  • Received : 2020.11.13
  • Accepted : 2021.03.03
  • Published : 2021.03.30
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Abstract

This study is an experiment to evaluate the anti-atopy efficacy of five kinds of natural plant essential oils; Artemisia annua L. (AA), Citrus junos Sieb. ex TANAKA (CJ), Chrysanthemum boreale Makino (CB), Pinus koraiensis (PK), and Pinus densiflora for. erecta (PD). Through Agar diffusion test, five species of native plant essential oils were treated in a total of four strains, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans. In order to invest the anti-inflammatory effect, five kinds of natural plant essential oils were treated in HaCaT cells-induced by TNF-α and IFN-γ (TI). AA, CJ, CB, PK and PD showed antibacterial effects on Candida albicans at a concentration of 10 mg/mL. We also found that the thymus and activation-regulated chemokine (TARC) expression was suppressed in 0.1 ㎍/mL of PK, 1 ㎍/mL of AA, CB, and PK. macrophage-derived chemokine (MDC) expression was suppressed in 1 ㎍/mL of AA and PK. IL-6 expression was suppressed in 0.1, 1 ㎍/mL of AA, PK in HaCaT cells. Hence it suggests that AA, CB, and PK have the anti-inflammatory effects, and it could contribute to atopic dermatitis relief by reducing the infiltration of immune cells to inflamed area.

본 연구는 개똥쑥(Artemisia annua L., AA), 유자(Citrus junos Sieb. ex TANAKA, CJ), 산국화(Chrysanthemum boreale Makino, CB), 잣나무(Pinus koraiensis, PK), 금강송(Pinus densiflora for. erecta, PD) 총 5 종의 자생식물 essential oil의 항 아토피 효능을 확인하기 위한 실험이다. 항균 효과를 확인하기 위해 자생식물 essential oil 5 종을 Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans 총 4 종류의 균에 처리했다. 항염증 효과를 확인하기 위해 TNF-α와 IFN-γ (TI)를 처리한 HaCaT 세포에 5 종의 자생식물 essential oil을 처리했다. AA, CJ, CB, PK, PD은 10 mg/mL 농도에서 Candida albicans에 대한 항균 효과를 나타냈다. AA (1 ㎍/mL), CB (1 ㎍/mL), PK (0.1, 1 ㎍/mL)에서 thymus and activation-regulated chemokine (TARC) 생성량이 억제되었고, AA 및 PK (1 ㎍/mL)에서 macrophage-derived chemokine (MDC) 생성량이 억제되었으며, AA (0.1, 1 ㎍/mL), PK (0.1, 1 ㎍/mL)에서 IL-6 생성량이 억제되었다. AA, CB, PK essential oil의 항균 및 항염증 효과가 확인되었으며, 이에 아토피 피부염 완화에 기여할 수 있음을 기대한다.

Keywords

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