Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Anti-obesity effects of an enzymatic extract of mandarin (Citrus unshiu) peel in 3T3-L1 adipocytes

감귤피 효소적 추출물의 지방세포에서의 항비만 효과

  • Received : 2020.12.10
  • Accepted : 2021.02.08
  • Published : 2021.04.30
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Abstract

Mandarin peel (MP) is a by-product of the processing of citrus juice or other products. This study aimed to investigate the potential anti-obesity effect of an enzymatic extract of MP on the inhibition of adipogenesis in 3T3-L1 adipocytes. The enzymatic extract (MPCE) was prepared using the commercial food-grade carbohydrase Celluclast. Lipid accumulation and triglyceride levels were significantly lower in MPCE-treated cells than in untreated cells. In addition, MPCE treatment reduced the protein expression levels of peroxisome proliferator-activated receptor-γ, CCAAT/enhancer-binding protein α, sterol regulatory element-binding protein 1, and fatty acid-binding protein 4. These results suggest that MPCE inhibits adipogenesis by downregulating the expression levels of adipogenesis-related proteins. Therefore, the current findings demonstrate that MPCE possesses potent anti-obesity properties and could be a potential ingredient in functional food industries.

본 연구는 감귤 가공공정 중에 발생하는 가공부산물인 감귤피의 산업적 이용성 및 유용성을 증대시키고자 효소적 추출법을 적용하여 추출물을 제조하고 항비만 활성을 측정하였다. 감귤피 효소적 추출물(MPCE)의 폴리페놀 함량은 이전의 연구에서 보고한 유기용매 추출물보다 높은 함량을 나타내었다. MPCE의 항비만 활성을 알아보기 위해 지방세포 분화억제에 미치는 영향을 확인한 결과 MPCE는 농도 의존적으로 지방세포의 분화 및 지방축적을 감소시켰으며 이는 세포독성에 의한 것이 아님을 세포생존율 측정을 통해 확인하였다. 또한 MPCE는 adipogenesis 관련 전사인자인 C/EBPα, SREBP-1, PPARγ, FABP4의 단백질 발현을 감소시켰다. 이러한 결과들을 통해 MPCE는 adipogenesis 관련 전사인자의 단백질 발현 억제하여 지방세포의 분화 및 지방축적을 억제하였으며, 이를 통해 항비만 효과를 나타낸다는 것을 확인하였다. 따라서 MPCE는 비만 예방 효능을 가진 기능성 식품 소재로서의 활용 가치가 있는 것으로 판단되며, 보다 명확한 항비만 효능을 확인하기 위해 추후 동물 실험 등에 대한 연구가 필요할 것으로 생각된다.

Keywords

References

  1. Ahn MS, Kim HJ, Seo MK. A study on the anti-oxidative and antimicrobial activities of the Citrus Unshju peel extracts. J. Korean Soc. Food Cult. 22: 454-461 (2007)
  2. Cefalu WT, Ye J, Wang ZQ. Efficacy of dietary supplementation with botanicals on carbohydrate metabolism in humans. Endocr. Metab. Immune Disord.-Drug Targets 8: 78-81 (2008) https://doi.org/10.2174/187153008784534376
  3. Horton JD, Goldstein JL, Brown MS. SREBPs: activators of the complete program of cholesterol and fatty acid synthesis in the liver. J. Clin. Invest. 109: 1125-1131 (2002) https://doi.org/10.1172/JCI15593
  4. Hsu CL, Yen GC. Effects of flavonoids and phenolic acids on the inhibition of adipogenesis in 3T3-L1 adipocytes. J. Agric. Food Chem. 55: 8404-8410 (2007) https://doi.org/10.1021/jf071695r
  5. Hwang DI, Choi IH, Kim DY, Park SM, Kim HB, Li YL, Lee HM. Inhibitory effects of Chrysanthemum boreale makino on 3T3-L1 preadipocyte differentiation and down-regulation of adipogenesis and lipogenesis. J. Life Sci. 29: 332-336 (2019) https://doi.org/10.5352/JLS.2019.29.3.332
  6. Hwang JH, Park KY, Oh YS, Lim SB. Phenolic compound content and antioxidant activity of citrus peels. J. Korean Soc. Food Sci. Nutr. 42: 153-160 (2013) https://doi.org/10.3746/JKFN.2013.42.2.153
  7. Hyon JS, Kang SM, Senevirathne M, Koh WJ, Yang TS, Oh MC, Oh CK, Jeon YJ, Kim SH. Antioxidative activities of extracts from dried Citrus sunki and C. unshiu peels. J. Korean Soc. Food Sci. Nutr. 39: 1-7 (2010) https://doi.org/10.3746/JKFN.2010.39.1.001
  8. Kang MC, Ding Y, Kim EA, Choi YK, de Araujo T, Heo SJ, Lee SH. Indole derivatives isolated from brown alga Sargassum thunbergii inhibit adipogenesis through AMPK activation in 3T3?L1 preadipocytes. Mar. Drugs 15: 119-129 (2017) https://doi.org/10.3390/md15040119
  9. Kim NH, Jegal J, Kim YN, Heo JD, Rho JR, Yang MH, Jeong EJ. Chokeberry extract and its active polyphenols suppress adipogenesis in 3T3-L1 adipocytes and modulates fat accumulation and insulin resistance in diet-induced obese mice. Nutrients. 10: 1734 (2018) https://doi.org/10.3390/nu10111734
  10. Kim HJ, Jun BS, Kim SK, Cha JY, Cho YS. Polyphenolic compound content and antioxidative activities by extracts from seed, sprout and flower of safflower (Carthamus tinctiorius L.). J. Korean Soc. Food Sci. Nutr. 29: 1127-1132 (2000)
  11. Kim HK, Kim JN, Han SN, Nam JH, Na HN, Ha TJ. Black soybean anthocyanins inhibits adipocyte differentiation in 3T3?L1 cells. Nutr. Res. 32: 770-777 (2012) https://doi.org/10.1016/j.nutres.2012.06.008
  12. Ko SC, Kang SM, Ahn G, Yang HP, Kim KN, Jeon YJ. Antioxidant activity of enzymatic extracts from Sargassum coreanum. J. Korean Soc. Food Sci. Nutr. 39: 494-499 (2010) https://doi.org/10.3746/JKFN.2010.39.4.494
  13. Lee WJ, Koh EH, Won JC, Kim MS, Park JY, Lee KU. Obesity: the role of hypothalamic AMP-activated protein kinase in body weight regulation. Int. J. Biochem. Cell Biol. 37: 2254-2259 (2005) https://doi.org/10.1016/j.biocel.2005.06.019
  14. Lee SH, Park MW, Han JS, Jeong Y, Kim M, Jeon YJ. Bioactive compounds extracted from Gamtae (Ecklonia cava) by using enzymatic hydrolysis, a potent α-glucosidase and α-amylase inhibitor, alleviates postprandial hyperglycemia in diabetic mice. Food Sci. Biotechol. 21: 1149-1155 (2012) https://doi.org/10.1007/s10068-012-0150-x
  15. Lee SH, Yang HW, Ding Y, Wang Y, Jeon YJ, Moon SH, Jeon BT, Sung SH. Anti-inflammatory effects of enzymatic hydrolysates of velvet antler in Raw 264.7 cells in vitro and zebrafish model. EXCLI J. 14: 1122-1132 (2015)
  16. Lim H, Seo J, Chang YH, Han BK, Jeong JK, Park SB, Choi HJ, Hwang J. Anti-obesity effects of Jeju Hallabong tangor (Citrus kiyomi X ponkan) peel extracts in 3T3-L1 adipocytes. J. Korean Soc. Food Sci. Nutr. 43: 1688-1694 (2014) https://doi.org/10.3746/JKFN.2014.43.11.1688
  17. Meda A, Lamien CE, Romito M, Millogo J, Nacoulma OG. Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chem. 9: 571-577 (2005)
  18. Moon HS, Chung CS, Lee HG, Kim TG, Choi YJ, Cho CS. Inhibitory effect of (-)-epigallocatechin-3-gallate on lipid accumulation of 3T3-L1 cells. Obesity 15: 2571-2582 (2007) https://doi.org/10.1038/oby.2007.309
  19. Park CH, Rhyu DY, Sharma BR, Yokozama T. Inhibition of preadipocyte differentiation and lipid accumulation by 7?O?galloyl?dsedoheptulose treatment in 3T3?L1 adipocytes. Biomed. Prev. Nutr. 3: 319-324 (2013) https://doi.org/10.1016/j.bionut.2013.03.012
  20. Rayalam S, Della-Fera MA, Baile CA. Phytochemicals and regulation of the adipocyte life cycle. J. Nutr. Biochem. 19: 717-726 (2008) https://doi.org/10.1016/j.jnutbio.2007.12.007
  21. Rosen E, Eguchi J, Xu Z. Transcriptional targets in adipocyte biology. Expert Opin. Ther. Targets 13: 975-986 (2009) https://doi.org/10.1517/14728220903039706
  22. Rosen ED, Sarraf P, Troy AE, Bradwin G, Moore K, Milstone DS, Spiegelman BM, Mortensen RM. PPAR is required for differentiation of adipose tissue in vivo and in vitro. Mol Cell. 4: 611-617 (1999) https://doi.org/10.1016/S1097-2765(00)80211-7
  23. Seo EY. Effects of (6)-gingerol, ginger component on adipocyte development and differentiation in 3T3-L1. J Nutr Health 48: 327-334 (2015) https://doi.org/10.4163/jnh.2015.48.4.327
  24. Seo J, Lim H, Chang YH, Park HR, Han BK, Jeong JK, Choi KS, Park SB, Choi HJ, Hwang J. Effects of Jeju Citrus unshiu peel extracts before and after bioconversion with cytolase on anti-inflammatory activity in RAW264.7 Cells. J. Korean Soc. Food Sci. Nutr. 44: 331-337 (2015) https://doi.org/10.3746/JKFN.2015.44.3.331
  25. Song YW, Moon KS, Cho Kim S. Antioxidant activity and nutrient content of ethanol and hot-water extracts of Citrus unshiu pomace. J. Korean Soc. Food Sci. Nutr. 42: 1345-1350 (2013) https://doi.org/10.3746/JKFN.2013.42.9.1345
  26. Wang X, Hai C. Redox modulation of adipocyte differentiation: hypothesis of "Redox Chain" and novel insights into intervention of adipogenesis and obesity. Free Radic. Biol. Med. 89: 99-125 (2015) https://doi.org/10.1016/j.freeradbiomed.2015.07.012
  27. Wang L, Lee WW, Yang HW, Ryu BM, Cui YR, Lee SC, Lee TG, Jeon YJ. Protective effect of water extract of citrus pomace against AAPH-induced oxidative stress in vitro in Vero cells and in vivo in zebrafish. Prev Nutr Food Sci 23: 301-308 (2018) https://doi.org/10.3746/pnf.2018.23.4.301
  28. World Health Organization (WHO). Obesity. Available from: https://www.who.int/news-room/facts-in-pictures/detail/6-facts-on-obesity. Accessed Feb. 10, 2020.