한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
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Barringtonia augusta Kurz 추출물의 항염증 및 항산화 효능 평가

Anti-inflammatory and antioxidant effects of Barringtonia augusta Kurz extract

  • 류수호 (경북대학교 식품공학부 식품응용공학전공) ;
  • 김민정 (경북대학교 식품공학부 식품응용공학전공) ;
  • ;
  • 정성근 (경북대학교 식품공학부 식품응용공학전공)
  • Ryu, Soo Ho (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Kim, Min Jeong (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Bach, Tran The (Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology (VAST)) ;
  • Jung, Sung Keun (School of Food Science and Biotechnology, Kyungpook National University)
  • 투고 : 2020.12.19
  • 심사 : 2021.02.09
  • 발행 : 2021.04.30
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초록

본 연구에서는 LPS로 유도된 RAW 264.7 대식세포에서 Barringtonia augusta Kurz 추출물의 항염증, 항산화 효능을 확인하였다. BKE는 LPS에 의한 NO와 ROS의 생성을 유의적으로 억제하였고, iNOS 발현 또한 억제하였다. 특히 IKK 매개 NF-κB pathway에서, IKK의 인산화 억제를 통하여, IκBα의 감소와 NF-κB의 인산화를 억제하여, NF-κB의 세포질에서 핵으로의 이동을 유의적으로 억제하였다. 이 논문은 BKE가 NO와 ROS의 생성과 iNOS의 발현을 IKK매개 NF-κB 인산화 억제를 통해 항염증 및 항산화 효과를 나타냄을 보여주었다. 이러한 결과를 바탕으로 BKE는 항염증, 항산화 기능성 식품 또는 의약품 소재로써 활용가치가 높을 것으로 기대된다.

Barringtonia augusta Kurz is a species of the genus Barringtonia. Although several studies have analyzed the biological activity of B. racemosa Roxb and B. acutangula, the anti-inflammatory and antioxidant effects of B. augusta extract (BKE) remain unclear. Therefore, in this study, we investigated the anti-inflammatory and antioxidant effects of BKE using lipopolysaccharide (LPS) and RAW 264.7. BKE suppressed LPS-induced nitric oxide (NO) and inducible NO synthase expression without affecting RAW 264.7 cell viability. Additionally, BKE showed 2,2-Diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging capacities and inhibited LPS-induced reactive oxygen species production in RAW 264.7 cells. BKE also suppressed LPS-induced phosphorylation of IκB kinase and nuclear factor kappa-B (NF-κB) and p65 translocation from the cytosol to the nucleus in RAW 264.7 cells. These results suggest that BKE is a possible novel material that exerts beneficial antioxidant and anti-inflammatory effects through the inhibition of NF-κB signaling pathways.

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