Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Physiological activities of ethanol extract of Allium tuberosum root

부추뿌리 추출물의 생리활성

  • Kim, Hyun-Soo (Department of Food science and Technology, Jungwon University)
  • 김현수 (중원대학교 식품공학과)
  • Received : 2021.01.04
  • Accepted : 2021.02.16
  • Published : 2021.04.30
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Abstract

This study investigated various physiological activities to examine the applicability of the functional materials of Allium tuberosum root extract. The A. tuberosum root extract showed a low cytotoxicity against murine melanoma B16F10 cells. It also showed high DPPH radical scavenging activity (ID50, 6.2 ㎍/mL), inhibited tyrosinase activity (ID50, 115.4 ㎍/mL), and decreased melanin content (ID50, 31.5 ㎍/mL). Treatment of B16F10 cells with A. tuberosum root extract suppressed the protein expression of tyrosinase in a dose-dependent manner. These findings suggest that A. tuberosum root extract inhibits melanin synthesis by suppressing intracellular tyrosinase expression. Additionally, A. tuberosum root extract inhibited elastase with an ID50 value of 145.1 ㎍/mL and contained isoquercitrin. These results indicate that A. tuberosum root extract is an appropriate natural material.

부추뿌리 추출물(Allium tuberosum root extract)에 대한 다양한 생리활성을 조사하여 천연물소재 가능성을 시험하였다. 부추뿌리 추출물은 멜라노마 세포에 대하여 낮은 세포독성을 나타내었으며 세포독성이 거의 없는 농도에서 부추뿌리 추출물 처리 시, 산화방지 활성(ID50, 6.2 ㎍/mL), 타이로시네이즈 활성저해(ID50, 115.4 ㎍/mL) 및 멜라닌 합성 저해(ID50, 31.5 ㎍/mL)를 나타내었다. 부추뿌리 추출물 농도 의존적으로 타이로시네이즈 발현이 억제가 관찰되었으며, 부추뿌리 추출물이 타이로시네이즈 단백질 발현을 억제시킴으로써 타이로시네이즈 활성저해 및 멜라닌 합성을 저하시키는 효과가 나타난 것으로 판단된다. 또한, 부추뿌리 추출물이 피부탄력 유지에 관련된 엘라스테이즈 활성저해(ID50, 145.1 ㎍/mL)를 보여주었으며 이소케르세틴(isoquercitrin)이 유효성분으로 확인되었다. 이와 같은 결과로 미루어 볼 때 부추뿌리 추출물은 기능성 소재에 활용하기 위한 효과적인 천연물 재료가 될 수 있다고 판단된다.

Keywords

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