Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Effects of Radiation Mutant Perilla frutescens var. crispa and Atractylodes macrocephala Koidzumi Complex Extract on the Mediators Related to Degenerative Arthritis

방사선 형질전환 차조기와 백출 복합추출물이 퇴행성관절염 관련 매개체에 미치는 영향

  • Sim, Boo-Yong (Division of Integrated Biotechnology, Joongbu University) ;
  • Joo, In-Hwan (College of Oriental Medicine, Daejeon University) ;
  • Kim, Sung-Kyu (SFC bio Co., LTD) ;
  • Ji, Joong-Gu (Department of Herbal Health & Pharmacy, Joongbu University)
  • 심부용 (중부대학교 바이오융합학부) ;
  • 주인환 (대전대학교 한의과대학) ;
  • 김성규 ((주)에스에프씨바이오) ;
  • 지중구 (중부대학교 한방보건제약학과)
  • Received : 2021.04.03
  • Accepted : 2021.04.26
  • Published : 2021.04.30
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Abstract

The present study aimed to evaluate the effects of radiation mutant Perilla frutescens var. crispa and Atractylodes macrocephala Koidzumi complex extract(Perilla frutescens var. crispa complex extract) on the mediators related to degenerative arthritis in a monosodium iodoacetate-induced rat model of degenerative arthritis. Perilla frutescens var. crispa complex extract was administered orally at doses of 25, 50 or 100 mg/kg/day for 2 weeks before direct injection of monosodium iodoacetate (3 mg/50 µl of 0.9% saline) into the intra-articular space of the rats' right knees. The rats subsequently received the same doses of oral Perilla frutescens var. crispa complex extract for another 4 weeks. It was evaluated that the treatment effects based on serum bio-markers, and morphological and histopathological analysis of the knee joints. Compared with those in negative control rats, the Perilla frutescens var. crispa complex extract treatments significantly reduced the serum levels of inflammation, bone metabolism markers (i.e., TNF-α, MMP-3, COX-2, PGE2, COMP, and Aggrecan). Otherwise, it was significantly increased the production of CTX-2 in cartilage absorption mediators. In addition, the Perilla frutescens var. crispa complex extract treatments effectively preserved the knee cartilage and synovial membrane. As a result, it indicates that the Perilla frutescens var. crispa complex extract improved degenerative arthritis symptoms. Thus, the Perilla frutescens var. crispa complex can be used in food material for the management of degenerative arthritis.

본 연구는 방사선 형질전환 차조기와 백출복합물(차조기 복합물)이 퇴행성관절염 관련 매개체에 미치는 영향을 확인하고자 MIA(monosodium iodoacetate)로 퇴행성관절염을 유도한 랫드로 평가하였다. 차조기 복합추출물을 2주 동안 25, 50, 100 mg/kg/day의 용량으로 경구 투여하고 랫드의 우측관절 내 공간에 MIA를 주입한 후 동일 용량을 4주 동안 지속 투여하였다. 이후, 혈청 바이오마커와 무릎 관절 분석의 형태학 및 조직병리학적 분석에 기초한 치료 효과를 평가하였다. 대조군 랫드와 비교하였을 때 차조기 복합추출물은 혈청 내 염증 및 골 대사 마커(TNF-α, MMP-3, COX-2, PGE2, COMP, Aggrecan)의 생성량을 유의하게 감소시켰다. 이와는 반대로 연골 흡수 매개체인 CTX-2 생성을 증가시켰으며, 방사선 형질전환 차조기는 무릎 연골과 활막을 효과적으로 보존하였다. 그 결과, 차조기 복합물은 퇴행성관절염 증상을 개선하였다. 따라서, 차조기 복합물은 퇴행성관절염 관리를 위한 식품소재로 사용될 수 있다.

Keywords

Acknowledgement

본 연구는 산업통상자원부와 한국산업기술진흥원이 지원하는 광역협력권산업육성사업(2020-P0006184)으로 수행된 연구 결과입니다.

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