Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
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ICT Convergenced Cascade-type Incubator for mass production of microalgae

미세조류 대량생산을 위한 ICT 융합 계단식 연속 배양 장치

  • Lee, Geon Woo (Department of Physics and Nano-science, Sun Moon University) ;
  • Lee, Yong Bok (Department of Physics and Nano-science, Sun Moon University) ;
  • Yoo, Yong Jin (Department of Physics and Nano-science, Sun Moon University) ;
  • Baek, Dong Hyun (Center for Next-Generation Semiconductor Technology, Sun Moon University) ;
  • Kim, Jin Woo (Department of Food Science, Sun Moon University) ;
  • Kim, Ho Seob (Department of Physics and Nano-science, Sun Moon University)
  • 이건우 (선문대학교 나노과학과) ;
  • 이영복 (선문대학교 나노과학과) ;
  • 유용진 (선문대학교 나노과학과) ;
  • 백동현 (선문대학교 차세대반도체기술연구소) ;
  • 김진우 (선문대학교 식품과학과) ;
  • 김호섭 (선문대학교 나노과학과)
  • Received : 2021.02.03
  • Accepted : 2021.05.07
  • Published : 2021.05.31
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Abstract

This study was undertaken to develop a cascade-type continuous culture system (CCCS) that combines both ICT and biotechnology (BT), for the mass production of microalgae. This system is capable of maintaining the essential culture conditions of pH, temperature, carbon dioxide, and illuminance control, which are key parameters for the growth of microalgae, and is economical for producing microalgae regardless of the season or location. It has the added advantage of providing stable and high productivity. In the current study, this system was applied to culture microalgae for 71 days, with subsequent analysis of the experimental data. The initial O.D. of the culture measured from incubator 1 was 0.006. On the 71st day of culture, the O.D.s obtained were 0.399 (incubator 1), 0.961 (incubator 2), 0.795 (incubator 3), and 0.438 (incubator 4), thereby confirming the establishment of continuous culture. Thus, we present a smart-farm based on ISMC (in-situ monitoring and control) for a mass culture method. We believe that this developed technology is suitable for commercialization, and has the potential to be applied to hydroponics-based cultivation of microalgae and cultivation of high-value-added medicinal plants as well as other plants used in functional foods, cosmetics, and medical materials.

본 연구 목적은 미세조류 대량생산을 위한 ICT와 BT기술이 융합된 계단식 연속 배양 시스템(CCCS)을 개발함에 있다. 본 시스템은 미세조류 성장에 필요한 주요 운전변수인 pH, 온도, 이산화탄소와 조도 제어를 통해 실시간 배양조건 유지를 통해 세포성장 증진과 계절과 장소에 관계없이 미세조류를 생산할 수 있어 경제성이 확보 된 시스템이다. 또한, 안정적이고 높은 생산성을 제공하는 장점을 갖는다. 본 연구에서는 이 시스템을 활용하여 71일간 미세조류를 배양하고 실험 데이터를 분석하였다. 그 결과, 배양초기 O.D.는 수조1에서 0.006로 측정되었으며 이후 배양 71일, O.D.는 수조1: 0.399, 수조2: 0.961, 수조3: 0.795 그리고 수조4: 0.438로 측정되었다. 따라서 배양기간 동안 연속적인 배양이 가능함이 확인되었다. 대량 배양 방식인 ISMC (In-situ monitoring and control)기반 스마트팜을 제시하고, 본 개발기술을 통해 미세조류 이외의 수경재배 기반의 약용식물 등에 적용하여 식품, 화장품 그리고 의료 소재용 고부가가치 식물 재배에도 적용이 가능한 상업화 적합 기술이라 사료된다.

Keywords

Acknowledgement

본 연구는 중소벤처기업부와 한국산업기술진흥원의 "지역특화산업육성사업(R&D P0004891)"으로 수행된 연구결과입니다.

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