한국균학회지 (The Korean Journal of Mycology)

  • 제49권1호
  • /
  • Pages.45-55
  • /
  • 2021
  • /
  • 0253-651X(pISSN)
  • /
  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
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Artomyces microsporus의 배양적 특성과 균사 적정 배양 조건 설정

Culture Characteristics and Optimal Conditions for Mycelial Growth of Artomyces microsporus

  • 민경진 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) ;
  • 이은지 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) ;
  • 박혜성 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) ;
  • 이찬중 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과)
  • Min, Gyeong-Jin (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Lee, Een-ji (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Park, Hea-sung (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Lee, Chan-Jung (Mushroom Science Division, National Institute of Horticultural and Herbal Science, RDA)
  • 투고 : 2021.02.26
  • 심사 : 2021.03.29
  • 발행 : 2021.03.31
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초록

본 연구에서는 A. microsporus의 최적 배양 조건을 구명하고자 하였다. A. microsporus 균사체의 효율적 배양을 위한 조건은 최적 배양 온도 25℃, pH 5.0, 탄소원 soluble starch 1%, 유기질소원 malt extract 1%, 무기질소원 NH4H2PO4 0.1%, 아미노산 asparatic acid 0.1% 조건으로 선발되었고 유기산, 무기염류 첨가 시 균사생육이 저조해지는 영향을 주어 선발되지 않았다. 새로이 SMNA로 명명한 A. microsporus의 최적 배지는 기존의 PDA 배지 상에서 배양한 결과와 비교하였을 때, 균사의 생육이 균일하고 안정적이며 배양 기간을 15.1% 정도 단축하는 효과가 있었다. SMNA 배지는 추후 A. microsporus의 균사체 배양 방법에 널리 활용될 수 있을 것으로 판단된다.

Artomyces microspora is a genus of coral fungi from the family Auriscalpiaceae that have sporophores which are clavarioid, profusely and pyxidately branched, and devoid of a conspicuous stipe. These fungi can be found in summer and fall. This study aimed to decipher fundamental information regarding optimal growth conditions of Artomyces microsporus mycelia, including pH, temperature, carbon sources, and nitrogen sources. Based on the assessment of colony diameter and mycelial density, the optimal culture medium, temperature, and pH for mycelial growth were found to be PDA, 25 ℃, and pH 5.0, respectively. Furthermore, the study revealed that the optimal carbon and nitrogen sources for mycelial growth were 1% soluble starch and 2% malt extract, respectively. The other suitable inorganic nitrogen sources were deemed to be 0.1% NH4H4PO4 and 0.1% aspartic acid.

키워드

과제정보

This work was supported by the research program of agricultural science and technology development (PJ014763) of the National Institute of Horticultural and Herbal Science.

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