Journal of the Korean Magnetic Resonance Society (한국자기공명학회논문지)

Korean Magnetic Resonance Society (한국자기공명학회)
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Comparison of metabolic profiling of Daphnia magna between HR-MAS NMR and solution NMR techniques

  • Kim, Seonghye (Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University) ;
  • Lee, Sujin (Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University) ;
  • Lee, Wonho (Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University) ;
  • Lee, Yujin (Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University) ;
  • Choi, Juyoung (Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University) ;
  • Lee, Hani (Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University) ;
  • Li, Youzhen (Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University) ;
  • Ha, Seulbin (Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University) ;
  • Kim, Suhkmann (Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University)
  • Received : 2021.06.16
  • Accepted : 2021.06.18
  • Published : 2021.06.20
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Abstract

Daphnia magna is used as target organism for environmental metabolomics. The metabolome of D. magna was studied with NMR spectroscopy. Most studies used the extract of D. magna, but the reproducibility cannot be obtained using extracted sample. In this study, lyophilized D. magna samples were analyzed with two different 1H NMR techniques, HR-MAS on intact tissues and solution NMR on extracted tissues. Samples were measured three times using 600 MHz NMR spectrometer. Metabolite extraction required more than twice as many D. magna, but the metabolite intensity was lower in solution NMR. In the spectra of HR-MAS NMR, the lipid signal was observed, but they did not interfere with metabolite profiling. We also confirmed the effect of swelling time on signal intensities of metabolites in HR-MAS NMR, and the results suggest that appropriate swelling should be used in lyophilized D. magna to improve the accuracy of metabolite profiles.

Keywords

Acknowledgement

This study was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (NRF-2020R1I1A2075016)

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