Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Protective effect of matcha green tea (Camellia sinensis) extract on high glucose- and oleic acid-induced hepatic inflammatory effect

고당 및 올레산으로 유도된 간세포에서의 염증반응에 대한 말차(Camellia sinensis) 추출물의 보호효과

  • Kim, Jong Min (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee, Uk (Division of Special Forest Resources, Department of Forest Bioresources, National Institute of Forest Science (NIFoS)) ;
  • Kang, Jin Yong (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Seon Kyeong (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Shin, Eun Jin (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Moon, Jong Hyun (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Min Ji (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Lee, Hyo Lim (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Kim, Gil Han (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Jeong, Hye Rin (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University) ;
  • Park, Hyo Won (Division of Special Forest Resources, Department of Forest Bioresources, National Institute of Forest Science (NIFoS)) ;
  • Kim, Jong Cheol (Institute of Hadong Green Tea) ;
  • Heo, Ho Jin (Division of Applied Life Science (BK21), Institute of Agriculture and Life Science, Gyeongsang National University)
  • 김종민 (경상국립대학교 응용생명과학부(BK21), 농업생명과학연구원) ;
  • 이욱 (국립산림과학원 산림특용자원연구과) ;
  • 강진용 (경상국립대학교 응용생명과학부(BK21), 농업생명과학연구원) ;
  • 박선경 (경상국립대학교 응용생명과학부(BK21), 농업생명과학연구원) ;
  • 신은진 (경상국립대학교 응용생명과학부(BK21), 농업생명과학연구원) ;
  • 문종현 (경상국립대학교 응용생명과학부(BK21), 농업생명과학연구원) ;
  • 김민지 (경상국립대학교 응용생명과학부(BK21), 농업생명과학연구원) ;
  • 이효림 (경상국립대학교 응용생명과학부(BK21), 농업생명과학연구원) ;
  • 김길한 (경상국립대학교 응용생명과학부(BK21), 농업생명과학연구원) ;
  • 정혜린 (경상국립대학교 응용생명과학부(BK21), 농업생명과학연구원) ;
  • 박효원 (국립산림과학원 산림특용자원연구과) ;
  • 김종철 ((재)하동녹차연구소) ;
  • 허호진 (경상국립대학교 응용생명과학부(BK21), 농업생명과학연구원)
  • Received : 2021.01.26
  • Accepted : 2021.03.29
  • Published : 2021.06.30
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Abstract

To evaluate hepatoprotective effects, the antioxidant capacities of matcha green tea extract (Camellia sinenesis) were compared to those of green leaf tea and the anti-inflammatory activities in HepG2 cells were investigated. Evaluation of the total phenolic and total flavonoid content, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, and inhibitory effect on lipid peroxidation indicated that the aqueous extract of matcha green tea presented significant catechin content and antioxidant capacity compared to those of green leaf tea. In addition, the extract had considerable inhibitory effects on α-glucosidase, α-amylase, and advanced glycation end-products. The matcha green tea extract significantly increased cell viability and reduced reactive oxygen species in H2O2- and high-glucose-treated HepG2 cells. Furthermore, in response to oleic acid-induced HepG2 cell injury, treatment with matcha green tea aqueous extract inhibited lipid accumulation and regulated the expression of inflammatory proteins such as p-JNK, p-Akt, p-GSK-3β, caspase-3, COX-2, iNOS, and TNF-α. Matcha green tea could be used as a functional material to ameliorate hepatic lipid accumulation and inflammation.

본 연구는 가루녹차의 가공품인 말차를 이용하여 항산화 평가와 고당으로 유도된 간세포에서의 간세포 보호효과와 지방간 개선 활성을 평가하기 위해 진행되었다. 말차의 catechin 함량과 총 페놀성 화합물 및 총 플라보노이드 화합물의 함량은 잎 녹차보다 우수한 함량을 나타내었으며, 라디칼 소거활성과 지질과산화물 억제활성 역시 잎 녹차보다 우수한 것을 확인하였다. 또한, 항당뇨 활성을 나타내는 α-glucosidase, α-amylase 및 최종당화산물에 대한 우수한 억제활성을 확인하였다. In vitro 간세포 보호효과를 평가한 결과, 말차는 효과적으로 산화적 스트레스 및 고당으로 인한 활성산소 생성 억제활성과 간세포 생존율 나타내었다. 또한, oleic acid로 유도된 지방 축적에 대한 말차 추출물의 억제활성을 확인하였으며, 지방간으로 인한 염증반응에 대한 조절을 확인하였다. 이러한 연구 결과를 바탕으로 녹차의 가공품 중 하나인 말차는 가공 전 잎 녹차에 비해 우수한 카테킨 함량과 항산화 활성을 지니며, 탄수화물의 섭취를 억제해주는 소화효소의 활성을 억제하여 간세포의 지질축적을 억제하는 데 도움을 줄 수 있을 뿐만 아니라 염증 감소에 도움을 줄 수 있는 소재로서의 가능성을 확인하였다.

Keywords

Acknowledgement

본 연구는 농림축산식품부의 재원으로 농림식품기술기획평가원의 수출전략기술개발사업(617072-5)의 지원을 받아 수행된 연구결과입니다.

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