DOI QR코드

DOI QR Code

Antioxidant effect of complex extracts from pine needle, green tea, and sea buckthorn leaves

솔잎, 녹차 및 비타민나무 잎 혼합추출물의 항산화효과

  • Ji, Hee Young (Department of Food Science and Nutrition, Daejin University) ;
  • Park, Min Gyu (Department of Food Science and Nutrition, Daejin University) ;
  • Joo, Shin Youn (Department of Food Science and Nutrition, Daejin University)
  • 지희영 (대진대학교 식품영양학과) ;
  • 박민규 (대진대학교 식품영양학과) ;
  • 주신윤 (대진대학교 식품영양학과)
  • Received : 2021.02.16
  • Accepted : 2021.05.07
  • Published : 2021.06.30

Abstract

This study investigated the antioxidant effects of complex extracts from pine needles (PN), green tea (GT), and sea buckthorn leaves (SL). Measurement of total polyphenol and flavonoid content, DPPH radical scavenging activity (DPPH), ABTS+ radical scavenging activity (ABTS), and reducing power (RP), showed that SL extract (SE) had the highest values. The superoxide radical scavenging activity (SSA) and nitrite scavenging activity (NSA) were the highest in the GT extract (GE). In most experiments, the PN extract (PE) showed higher activity in complex extracts than in single extracts. The combination of GT and SL led to a higher activity than that exhibited by GT (DPPH and ABTS at 300 ppm, RP at 100 and 500 ppm) and SL (SSA at 100 ppm and NSA at 300 ppm) alone. These results suggest that the combination of PE, GE, and SE may be a useful functional food material in the food industry.

본 연구에서는 솔잎, 녹차 및 비타민나무 잎의 추출물과 그 혼합추출물의 항산화 활성을 조사하였다. 시료 7종의 총 폴리페놀 및 총 플라보노이드 함량은 SE가 가장 높게 나타났다. DPPH, ABTS+ radical 소거능 및 환원력은 SE가 가장 높은 활성을 보였고, superoxide radical 소거능과 아질산염은 GE의 활성이 가장 높았다. PE는 대부분의 실험에서 녹차 및 비타민나무 잎과의 혼합추출물이 단일추출물에 비해 높은 활성을 보였다. GE는 DPPH radical 소거능(300 ppm), ABTS+ radical 소거능(300 ppm) 및 환원력(100 및 500 ppm)에서 비타민나무 잎과 혼합추출하면 그 활성이 높아졌고, SE는 superoxide radical 소거능(100 ppm)과 아질산염 소거능(300 ppm)에서 녹차와 혼합추출하면 활성이 높아지는 경향을 보였다. 이상의 결과에서 PE는 단일 및 혼합추출물 7종 중에서 가장 낮은 활성을 나타낸 시료로 녹차 및 비타민나무 잎과 함께 혼합(PGSE)하여 사용한다면 솔잎의 낮은 항산화 활성을 개선시킬 수 있을 것으로 사료된다. 또한 본 연구 결과는 솔잎, 녹차 및 비타민나무 잎을 이용한 건강 기능성 식품 산업에서 기초자료로 활용할 수 있을 것으로 생각된다.

Keywords

References

  1. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature. 181: 1199-1200 (1958) https://doi.org/10.1038/1811199a0
  2. Byun EB, Kim MJ, Kim SJ, Oh NS, Park SH, Kim WS, Song HY, Han JM, Kim KW, Byun EH. Antioxidant activity and neuroprotective effects of ethanol extracts from the core of Diospyros kaki. Korean J. Food Sci. Technol. 52: 60-66 (2020)
  3. Cheng SJ, Gao YN, Ho CT, Wang ZY. Studies on anti mutagenicity and anti carcinogenicity of green tea antioxidant. Food Sci. Ind. 22: 61-66 (1989)
  4. Cheon WY, Seo DY, Kim YH. Antioxidative and hepatocyte protective effects of guava (Psidium guajava L.) leaves cultivated in Korea. Korean J. Food Nutr. 32: 033-040 (2019) https://doi.org/10.9799/KSFAN.2019.32.1.033
  5. Cho BO, Yin HH, Shin JY, Fang CZ, Chang CD, Jang SI. Antiatopic effects of mixed extracts from date plum, persimmon, and mulberry leaves. J. Korean Soc. Food Sci. Nutr. 45: 501-509 (2016) https://doi.org/10.3746/JKFN.2016.45.4.501
  6. Chun YS, Kwon DY. The influence of blending tea and Crataegi Fructus on antioxidant activities and sensory evaluation. J. Tea Culture & Industry Studies. 33: 263-280 (2016) https://doi.org/10.21483/qwoaud.33..201609.263
  7. Dewanto V, Wu X, Liu RH. Processed sweet corn has higher antioxidant activity. J. Agric. Food Chem. 50: 4959-4964 (2002) https://doi.org/10.1021/jf0255937
  8. Elzaawely AA, Xuan TD, Koyama H, Tawata S. Antioxidant activity and contents of essential oil and phenolic compounds in flowers and seeds of Alpinia zerumbet (Pers.) B.L. Burtt. & R.M. Sm. Food Chem. 104: 1648-1653 (2007) https://doi.org/10.1016/j.foodchem.2007.03.016
  9. Ganju L, Padwad Y, Singh R. Anti-inflammatory activity of seabuckthorn (Hippophae rhamnoides) leaves. Int. Immunopharmacol. 5: 1675-1684 (2005) https://doi.org/10.1016/j.intimp.2005.03.017
  10. Gu YR, Hong JH. Physicochemical characteristics and physiological activities of mixture extracts from Liriope platyphylla, Schizandra chinensis, and Panax ginseng C.A. Meyer. Korean J. Food Preserv. 24: 431-439 (2017) https://doi.org/10.11002/KJFP.2017.24.3.431
  11. Hidalgo M, Sanchez-Moreno C, de Pascual-Teresa S. Flavonoid-flavonoid interaction and its effect on their antioxidant activity. Food Chem. 121: 691-696 (2010) https://doi.org/10.1016/j.foodchem.2009.12.097
  12. Jeong KH, Ji YS, Kil KJ, Yoo JH. Antioxidant effects of ethanol extracts from different parts of Portulacae Herba. Kor. J. Herbol. 34: 59-65 (2019)
  13. Jeong SJ, Shim HR, Lee JS, Nam HS, Lee HG. Antioxidant and synergistic activities of fruit and vegetable concentrates. Korean J. Food Sci. Technol. 47: 240-245 (2015) https://doi.org/10.9721/KJFST.2015.47.2.240
  14. Jin SY, Han YS, Joo NM. Optimization of iced cookies with the addition of pine leaf powder. Korean J. Food Cookery Sci. 22: 164-172 (2006)
  15. Kang HJ, Kim HS, Jeon IH, Mok JY, Han KS, Jang SI. Effect of antioxidant and blood flow improvement of grape leaf extract and resveratrol from vitis romaneti. J. Kor. Soc. Fod. Sci. Nutr. 42: 1736-1743 (2013) https://doi.org/10.3746/jkfn.2013.42.11.1736
  16. Kang ST, Yoo UH, Nam KH, Kang JY, Oh KS. Antioxidative effects of green tea extract on the oxidation of anchovy oil. J. Agric. Life Sci. 41: 47-53 (2007)
  17. Kato H, Le IE, Chuyen NV, Kim SB, Hayase F. Inhibition of nitrosamine formation by nondialyzable melanoidins. Agric. Biol. Chem. 51: 1333-1338 (1987) https://doi.org/10.1271/bbb1961.51.1333
  18. Kim KC, Kim JS. Physiological activity of the extract from dolwoe (Gynostemma pentaphyllum makino) leaves tea by different ethanol concentrations. J. Plant Biotechnol. 46: 37-44 (2019) https://doi.org/10.5010/JPB.2019.46.1.037
  19. Kim IY, Lee JY, Jeong YH. Antioxidant activities of Rumex crispus L. root extracts and fractions. J. Korean Soc. Food Sci. Nutr. 47: 1234-1241 (2018) https://doi.org/10.3746/jkfn.2018.47.12.1234
  20. Kim KM, Park MH, Kim KH, Im SH, Park YH, Kim YN. Analysis of chemical composition and in vitro antioxidant properties of extracts from sea buckthorn (Hippophae rhaminoides). J. Appl. Biol. Chem. 52: 58-64 (2009) https://doi.org/10.3839/jabc.2009.011
  21. Kim YS, Shin DH. Volatile components and antibacterial effect of pine needle (Pinus densiflora S. and Z.) extracts. Food Microbiol. 22: 37-45 (2005) https://doi.org/10.1016/j.fm.2004.05.002
  22. Kwon HY, Choi SI, Han XG, Men X, Jang GW, Choi YE, Kang JC, Cho JH, Lee OH. Antioxidant components and antioxidant activities of mixtures with Sasa quelpaertensis Nakai and Ficus erecta var. sieboldii. Korean J. Food Sci. Technol. 52: 369-376 (2020) https://doi.org/10.9721/KJFST.2020.52.4.369
  23. Lee YC, Hwang KH, Han DH. Composition of Opuntia ficus-india. Korean J. Food Sci. Technol. 29: 847-853 (1997)
  24. Lee SG, Jeong HJ, Lee EJ, Kim JB, Choi SW. Antioxidant and anti-inflammatory activities of ethanol extracts from medicinal herb mixtures. Korean J. Food Sci. Technol. 43: 200-205 (2011) https://doi.org/10.9721/KJFST.2011.43.2.200
  25. Lee DS, Kim KH, Yook HS. Antioxidant effects of fractional extracts from strawberry (Fragaria ananassa var. 'Seolhyang') leaves. J. Korean Soc. Food Sci. Nutr. 47: 263-270 (2018a) https://doi.org/10.3746/jkfn.2018.47.3.263
  26. Lee SY, Shon JY, Kang KO. Nutritional components and antioxidant activities of sea buckthron (Hippophae rhamnoides L.) leaf and berry extracts. J. East Asian Soc. Diet Life. 28: 31-39 (2018b) https://doi.org/10.17495/easdl.2018.2.28.1.31
  27. Mineo S, Syuji W, Toshimitu H, Munehisa A, Kenjiro J, Seiji O, Saburo Y, Naokata M. Studies on hypoglycemic constituents of Japanese tea. Yakugaku Zasshi. 108: 964-970 (1988) https://doi.org/10.1248/yakushi1947.108.10_964
  28. Moon GS, Kwon TW, Ryu SH. Comparison of antioxidative activities of soybean components by different assays. Korea Soybean Digest. 20: 28-36 (2003)
  29. Pyo YH, Lee TC, Logendra L, Rogen RT. Antioxidant activity and phenolic compounds of swiss chard (Beta vulgaris subspecies cycla) extracts. Food Chem. 85: 19-26 (2004) https://doi.org/10.1016/S0308-8146(03)00294-2
  30. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26: 1231-1237 (1999) https://doi.org/10.1016/S0891-5849(98)00315-3
  31. Roh HJ, Shin YS, Lee KS, Shin MK. Effect of water extract of green tea on the quality and shelf life of cooked rice. Korean J. Food Sci. Technol. 28: 417-420 (1996)
  32. Seifried HE, Anderson DE, Fisher EI, Milner JA. A review of the interaction among dietary antioxidants and reactive oxygen species. J. Nutr. Biochem. 18: 567-579 (2007) https://doi.org/10.1016/j.jnutbio.2006.10.007
  33. Shin CM, Choi YJ. Effect of green tea extract on lipid oxidation of water/horse oil emulsion. J. Korea Acad. Industr. Coop. Soc. 19: 407-414 (2018) https://doi.org/10.5762/KAIS.2018.19.7.407
  34. Tiffany TYG, Stefan C, Arnie H. Effect of drying on the nutraceutical quality of sea buckthorn leaves. J. Food Sci. 70: 514-518 (2005)
  35. Wang S, Meckling KA, Marcone MF, Kakuda Y, Tsao R. Synergistic, additive, and antagonistic effects of food mixtures on total antioxidant capacities. J. Agric. Food Chem. 59: 960-968 (2011) https://doi.org/10.1021/jf1040977
  36. Wang J, Yuan X, Jin Z, Tian Y, Song H. Free radical and reactive oxygen species scavenging activities of peant skins extract. Food Chem. 104: 242-250 (2007) https://doi.org/10.1016/j.foodchem.2006.11.035
  37. Wong JY, Chye FY. Antioxidant properties of selected tropical wild edible mushrooms. J. Food Compos. Ana. 122: 269-277 (2009) https://doi.org/10.1016/j.jfca.2008.11.021
  38. Yin HH, Cho BO, Lee HS, Chu JI, Jang SI. Synergistic effects of grape branch and Pleurotus eryngii extract combination against inflammation on activated mast cells and atopic dermatitis-like skin lesions in mice. Korean J. Food Sci. Technol. 48: 582-589 (2016) https://doi.org/10.9721/KJFST.2016.48.6.582