Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
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Regulatory Effect of Cannabidiol (CBD) on Decreased β-Catenin Expression in Alopecia Models by Testosterone and PMA Treatment in Dermal Papilla Cells

  • Park, Yoon-Jong (Department of Biological Sciences, College of Natural Sciences, Kangwon National University) ;
  • Ryu, Jae-Min (Department of Biological Sciences, College of Natural Sciences, Kangwon National University) ;
  • Na, Han-Heom (Department of Biological Sciences, College of Natural Sciences, Kangwon National University) ;
  • Jung, Hyun-Suk (Kangwon Center for System Imaging, Kangwon National University) ;
  • Kim, Bokhye (Kangwon Center for System Imaging, Kangwon National University) ;
  • Park, Jin-Sung (Korean Pharmacopuncture Institute) ;
  • Ahn, Byung-Soo (Korean Pharmacopuncture Institute) ;
  • Kim, Keun-Cheol (Department of Biological Sciences, College of Natural Sciences, Kangwon National University)
  • Received : 2021.05.30
  • Accepted : 2021.06.04
  • Published : 2021.06.30
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Abstract

Objectives: The hair follicle is composed of more than 20 kinds of cells, and mesoderm derived dermal papilla cells and keratinocytes cooperatively contribute hair growth via Wnt/β-catenin signaling pathway. We are to investigate β-catenin expression and regulatory mechanism by CBD in alopecia hair tissues and dermal papilla cells. Methods: We performed structural and anatomical analyses on alopecia patients derived hair tissues using microscopes. Pharmacological effect of CBD was evaluated by β-catenin expression using RT-PCR and immunostaining experiment. Results: Morphological deformation and loss of cell numbers in hair shaft were observed in alopecia hair tissues. IHC experiment showed that loss of β-catenin expression was shown in inner shaft of the alopecia hair tissues, indicating that β-catenin expression is a key regulatory function during alopecia progression. Consistently, β-catenin expression was decreased in testosterone or PMA treated dermal papilla cells, suggesting that those treatments are referred as a model on molecular mechanism of alopecia using dermal papilla cells. RT-PCR and immunostaining experiments showed that β-catenin expression was decreased in RNA level, as well as decreased β-catenin protein might be resulted from ubiquitination. However, CBD treatment has no changes in gene expression including β-catenin, but the decreased β-catenin expression by testosterone or PMA was restored by CBD pretreatment, suggesting that potential regulatory effect on alopecia induction of testosterone and PMA. Conclusion: CBD might have a modulating function on alopecia caused by hormonal or excess of signaling pathway, and be a promising application for on alopecia treatment.

Keywords

Acknowledgement

This work was supported by fund provided from Chuncheon Bioindustry Foundation (CBF), Chuncheon City, Kangwon-Do, Republic of Korea.

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