Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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In vitro hepatocyte inflammation by chaparral extract

Chaparral 추출물에 의한 in vitro 간세포 염증반응

  • Kim, Ilrang (Department of Food and Nutrition, Ulsan College)
  • 김일낭 (울산과학대학교 식품영양학과)
  • Received : 2021.02.22
  • Accepted : 2021.05.24
  • Published : 2021.06.30
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Abstract

In this study, the hepatotoxic mechanism of chaparral (Larrea tridentata) was investigated through in vitro experiments that measured cell death, inflammatory cytokine secretion, and intracellular fat accumulation by treating HepG2 hepatocytes with a 70% ethanol extract of chaparral at concentrations ranging from 0.001 to 100 ㎍/mL. Cell death was observed after treatment with chaparral extract at concentrations of 1-100 ㎍/mL (p<0.05). The secretion of the inflammatory cytokines, interleukin-8 and macrophage-colony stimulating factor, and fat accumulation were significantly increased even at a concentration of 0.1 ㎍/mL, which was 10 times lower than the observed concentration resulting in cell death (p<0.05). Hepatitis caused by inflammatory cytokine secretion and fat accumulation was shown to be a form of hepatotoxicity induced by chaparral extract. Hepatitis was expressed at a concentration lower than that causing serious toxicity such as cell death, suggesting that hepatotoxicity, including hepatitis, may be caused by ingestion of low concentrations of chaparral.

본 연구에서는 chaparral 70% 에탄올 추출물을 0.01-100 ㎍/mL의 농도로 HepG2 간세포에 처리하여 세포 사멸, 염증성 사이토카인 분비, 세포 내 지방 축적을 측정하는 in vitro 실험을 통해 chaparral의 간독성 기전을 조사하였다. Chaparral 추출물 처리에 의해 1-100 ㎍/mL의 농도에서 세포 사멸이 관찰되었으며, 염증성 사이토카인인 IL-8과 M-CSF의 분비 및 지방 축적은 10배 더 낮은 농도부터인 0.1-100 ㎍/mL에서 유의적으로 증가하였다(p<0.05). 본 연구결과에서 염증성 사이토카인 분비 및 지방 축적을 통한 간세포 염증은 chaparral 추출물에 의해 유발되는 간독성의 한 형태로 나타났다. 또한 간염 형태의 간독성은 세포 사멸이라는 심각한 독성을 야기시키는 농도보다 낮은 농도에서 발현되어 저농도의 chaparral 섭취에 의해 간염과 같은 간독성이 초래될 수 있음을 시사한다.

Keywords

References

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